NADPH Oxidase Activity and Cytochromeb558Content of Human Epstein-Barr-Virus-Transformed B Lymphocytes Correlate with Expression of Genes Encoding Components of the Oxidase System

1998 ◽  
Vol 360 (2) ◽  
pp. 158-164 ◽  
Author(s):  
Antonio Condino-Neto ◽  
Peter E. Newburger
Keyword(s):  
Nadph Oxidase ◽  
B Lymphocytes ◽  
Oxidase Activity ◽  
Epstein Barr Virus ◽  
Oxidase System ◽  
Nadph Oxidase Activity ◽  
Barr Virus ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Epstein Barr

scholarly journals NADPH oxidase of Epstein-Barr-virus immortalized B lymphocytes.

2001 ◽  
Vol 268 (19) ◽  
pp. 5197-5208 ◽  
Author(s):  
Marie-Hélène Paclet ◽  
Anthony W. Coleman ◽  
James Burritt ◽  
Françoise Morel
Keyword(s):  
Nadph Oxidase ◽  
B Lymphocytes ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Low NADPH oxidase activity in Epstein-Barr-virus-immortalized B-lymphocytes is due to a post-transcriptional block in expression of cytochrome b558

1995 ◽  
Vol 306 (1) ◽  
pp. 141-145 ◽  
Author(s):  
M Chetty ◽  
A J Thrasher ◽  
A Abo ◽  
C M Casimir
Keyword(s):  
Nadph Oxidase ◽  
B Cell ◽  
B Lymphocytes ◽  
Epstein Barr Virus ◽  
Hl60 Cells ◽  
Cytochrome B558 ◽  
Barr Virus ◽  
Generating Capacity ◽  
Cell Cytosol ◽  
Epstein Barr

The NADPH oxidase of phagocytes is known to be expressed in Epstein-Barr-virus-transformed B-lymphocytes, albeit at levels only approx. 5% of those found in neutrophils. We have investigated the basis of this low level of expression and find that all four specific components of the NADPH oxidase are expressed in B-lymphocytes, but only p47-phox protein attains levels equivalent with those found in neutrophils. This component was shown to phosphorylate and translocate to the membrane normally on activation. The other cytosolic component, p67-phox, did show a deficit, and by supplementing a B-cell cytosol extract with recombinant p67-phox, this was shown to account for the somewhat reduced activity of B-cell cytosol in a cell-free oxidase system. The cell-free analysis also clearly located the major deficiency in superoxide-generating capacity of B-lymphocytes to the membrane. Western blotting of membrane proteins revealed major reductions in the amount of cytochrome b558. Analysis of the levels of mRNA for both subunits of cytochrome b558, however, showed levels greater than expected. Significantly more mRNA for gp91-phox was present in B-cells than in undifferentiated HL60 cells, although it was not quite as abundant as in differentiated HL60 cells, which are capable of producing large amounts of superoxide. We conclude that the failure of B-lymphocytes to generate amounts of superoxide equivalent to those generated by neutrophils is primarily due to a post-transcriptionally determined block to the accumulation of cytochrome b558.

scholarly journals A Locus Encompassing the Epstein-Barr Virus bglf4 Kinase Regulates Expression of Genes Encoding Viral Structural Proteins

2014 ◽  
Vol 10 (8) ◽  
pp. e1004307 ◽  
Author(s):  
Ayman El-Guindy ◽  
Francesc Lopez-Giraldez ◽  
Henri-Jacques Delecluse ◽  
Jessica McKenzie ◽  
George Miller
Keyword(s):  
Epstein Barr Virus ◽  
Structural Proteins ◽  
Barr Virus ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Epstein Barr ◽  
Viral Structural Proteins

scholarly journals Immunization with Epstein–Barr Virus Core Fusion Machinery Envelope Proteins Elicit High Titers of Neutralizing Activities and Protect Humanized Mice from Lethal Dose EBV Challenge

Vaccines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 285
Author(s):  
Xinle Cui ◽  
Zhouhong Cao ◽  
Yuriko Ishikawa ◽  
Sara Cui ◽  
Ken-Ichi Imadome ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
B Lymphocytes ◽  
Neutralizing Antibodies ◽  
Epstein Barr Virus ◽  
Lethal Dose ◽  
Envelope Proteins ◽  
Humanized Mice ◽  
Target Cells ◽  
Barr Virus ◽  
Epstein Barr

Epstein–Barr virus (EBV) is the primary cause of infectious mononucleosis and is strongly implicated in the etiology of multiple lymphoid and epithelial cancers. EBV core fusion machinery envelope proteins gH/gL and gB coordinately mediate EBV fusion and entry into its target cells, B lymphocytes and epithelial cells, suggesting these proteins could induce antibodies that prevent EBV infection. We previously reported that the immunization of rabbits with recombinant EBV gH/gL or trimeric gB each induced markedly higher serum EBV-neutralizing titers for B lymphocytes than that of the leading EBV vaccine candidate gp350. In this study, we demonstrated that immunization of rabbits with EBV core fusion machinery proteins induced high titer EBV neutralizing antibodies for both B lymphocytes and epithelial cells, and EBV gH/gL in combination with EBV trimeric gB elicited strong synergistic EBV neutralizing activities. Furthermore, the immune sera from rabbits immunized with EBV gH/gL or trimeric gB demonstrated strong passive immune protection of humanized mice from lethal dose EBV challenge, partially or completely prevented death respectively, and markedly decreased the EBV load in peripheral blood of humanized mice. These data strongly suggest the combination of EBV core fusion machinery envelope proteins gH/gL and trimeric gB is a promising EBV prophylactic vaccine.

scholarly journals Activated or dominant inhibitory mutants of Rap1A decrease the oxidative burst of Epstein-Barr virus-transformed human B lymphocytes.

1994 ◽  
Vol 269 (29) ◽  
pp. 18743-18746 ◽  
Author(s):  
F.E. Maly ◽  
L.A. Quilliam ◽  
O. Dorseuil ◽  
C.J. Der ◽  
G.M. Bokoch
Keyword(s):  
B Lymphocytes ◽  
Oxidative Burst ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals The interplay between Epstein–Barr virus and B lymphocytes: implications for infection, immunity, and disease

2014 ◽  
Vol 58 (2-3) ◽  
pp. 268-276 ◽  
Author(s):  
Olivia L. Hatton ◽  
Aleishia Harris-Arnold ◽  
Steven Schaffert ◽  
Sheri M. Krams ◽  
Olivia M. Martinez
Keyword(s):  
B Lymphocytes ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

Epstein-Barr Virus Transformation of B Lymphocytes: Molecular Pathogenesis

1989 ◽  
pp. 3-15
Author(s):  
David Liebowitz ◽  
Elliott Kieff ◽  
Jeffery Sample ◽  
Mark Birkenbach ◽  
Fred Wang
Keyword(s):  
B Lymphocytes ◽  
Epstein Barr Virus ◽  
Molecular Pathogenesis ◽  
Barr Virus ◽  
Virus Transformation ◽  
Epstein Barr
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