Partial Characterization of the Active Site Human Platelet cAMP Phosphodiesterase, PDE3A, by Site-Directed Mutagenesis

Peter P. Cheung; L. Yu; Hong Zhang; Robert W. Colman

doi:10.1006/abbi.1998.0915

Partial Characterization of the Active Site Human Platelet cAMP Phosphodiesterase, PDE3A, by Site-Directed Mutagenesis

Archives of Biochemistry and Biophysics ◽

10.1006/abbi.1998.0915 ◽

1998 ◽

Vol 360 (1) ◽

pp. 99-104 ◽

Cited By ~ 14

Author(s):

Peter P. Cheung ◽

L. Yu ◽

Hong Zhang ◽

Robert W. Colman

Keyword(s):

Active Site ◽

Human Platelet ◽

Site Directed Mutagenesis ◽

Partial Characterization ◽

Directed Mutagenesis ◽

Camp Phosphodiesterase

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Construction and Characterization of Mutant Water-Soluble PQQ Glucose Dehydrogenases with Altered Km Values—Site-Directed Mutagenesis Studies on the Putative Active Site

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1999.1157 ◽

1999 ◽

Vol 264 (3) ◽

pp. 820-824 ◽

Cited By ~ 37

Author(s):

Satoshi Igarashi ◽

Takafumi Ohtera ◽

Hiromi Yoshida ◽

Arief Budi Witarto ◽

Koji Sode

Keyword(s):

Active Site ◽

Water Soluble ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Putative Active Site

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Characterization of phosphate binding in the active site of barnase by site-directed mutagenesis and NMR

Biochemistry ◽

10.1021/bi00111a022 ◽

1991 ◽

Vol 30 (47) ◽

pp. 11348-11356 ◽

Cited By ~ 23

Author(s):

Elizabeth M. Meiering ◽

Mark Bycroft ◽

Alan R. Fersht

Keyword(s):

Active Site ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Phosphate Binding

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6-Pyruvoyl Tetrahydropterin Synthase, An Enzyme With a Novel Type of Active Site Involving Both Zinc Binding and an Intersubunit Catalytic Triad Motif; Site-directed Mutagenesis of the Proposed Active Center, Characterization of the Metal Binding Site and Modelling of substrate Binding

Journal of Molecular Biology ◽

10.1006/jmbi.1995.0558 ◽

1995 ◽

Vol 253 (2) ◽

pp. 358-369 ◽

Cited By ~ 39

Author(s):

Daniel M. Bürgisser ◽

Beat Thöny ◽

Udo Redweik ◽

Daniel Hess ◽

Claus W. Heizmann ◽

...

Keyword(s):

Binding Site ◽

Active Site ◽

Metal Binding ◽

Catalytic Triad ◽

Site Directed Mutagenesis ◽

Zinc Binding ◽

Directed Mutagenesis ◽

Metal Binding Site ◽

Motif Site

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Characterization of lysyl residues of NADH-cytochrome b5 reductase implicated in charge-pairing with active-site carboxyl residues of cytochrome b5 by site-directed mutagenesis of an expression vector for the flavoprotein.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)45910-8 ◽

1992 ◽

Vol 267 (4) ◽

pp. 2519-2523 ◽

Cited By ~ 1

Author(s):

P Strittmatter ◽

J M Kittler ◽

J E Coghill ◽

J Ozols

Keyword(s):

Active Site ◽

Expression Vector ◽

Cytochrome B5 ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Cytochrome B5 Reductase ◽

Nadh Cytochrome

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Site-directed mutagenesis of conserved serines in rat histidase. Identification of serine 254 as an essential active site residue.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)47009-3 ◽

1994 ◽

Vol 269 (44) ◽

pp. 27473-27477

Author(s):

R G Taylor ◽

R R McInnes

Keyword(s):

Active Site ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Active Site Residue

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Characterization of the phosphotransferase domain of casein kinase II by site-directed mutagenesis and expression in Escherichia coli.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)35921-0 ◽

1992 ◽

Vol 267 (33) ◽

pp. 23894-23902

Author(s):

R Jakobi ◽

J.A. Traugh

Keyword(s):

Escherichia Coli ◽

Casein Kinase Ii ◽

Casein Kinase ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Expression In Escherichia Coli

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Heterologous expression of human prostatic acid phosphatase and site-directed mutagenesis of the enzyme active site.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)37063-1 ◽

1994 ◽

Vol 269 (12) ◽

pp. 8971-8978

Author(s):

K. Ostanin ◽

A. Saeed ◽

R.L. Van Etten

Keyword(s):

Acid Phosphatase ◽

Heterologous Expression ◽

Active Site ◽

Prostatic Acid Phosphatase ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Enzyme Active Site

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Characterization of regions of fibronectin besides the arginine-glycine-aspartic acid sequence required for adhesive function of the cell-binding domain using site-directed mutagenesis

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)98498-x ◽

1991 ◽

Vol 266 (24) ◽

pp. 15938-15943 ◽

Cited By ~ 3

Author(s):

S. Aota ◽

T. Nagai ◽

K.M. Yamada

Keyword(s):

Aspartic Acid ◽

Binding Domain ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Cell Binding ◽

Arginine Glycine Aspartic Acid ◽

Cell Binding Domain

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Characterization of cathepsin B specificity by site-directed mutagenesis. Importance of Glu245 in the S2-P2 specificity for arginine and its role in transition state stabilization.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)54140-5 ◽

1993 ◽

Vol 268 (1) ◽

pp. 235-240

Author(s):

S. Hasnain ◽

T. Hirama ◽

C.P. Huber ◽

P. Mason ◽

J.S. Mort

Keyword(s):

Transition State ◽

Cathepsin B ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Transition State Stabilization

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CHEMICAL FUNCTIONS OF AMINO ACID RESIDUES IN THE ACTIVE SITE OF PARAHYDROXYBENZOATE HYDROXYLASE AS STUDIED BY SITE DIRECTED MUTAGENESIS AND BIOPHYSICAL TECHNIQUES

Flavins and Flavoproteins 1990 ◽

10.1515/9783110855425-043 ◽

1991 ◽

pp. 219-230

Author(s):

Barrie Entsch ◽

Bruce A. Palfey ◽

David P. Ballou ◽

Vincent Massey

Keyword(s):

Amino Acid ◽

Active Site ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Amino Acid Residues ◽

Biophysical Techniques

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