Identification of the Uridine Diphosphate Glucuronosyltransferase Isoform UGT1A6 in Rat Brain and in Primary Cultures of Neurons and Astrocytes

1998 ◽  
Vol 358 (1) ◽  
pp. 63-67 ◽  
Author(s):  
Funmilayo G. Suleman ◽  
Amr Abid ◽  
Daniela Gradinaru ◽  
Jean-Luc Daval ◽  
Jacques Magdalou ◽  
...  
Keyword(s):  
Rat Brain ◽  
Primary Cultures ◽  
Uridine Diphosphate ◽  
Uridine Diphosphate Glucuronosyltransferase

Uridine diphosphate-glucuronosyltransferase activities in rat brain microsomes

1993 ◽  
Vol 161 (2) ◽  
pp. 219-222 ◽  
Author(s):  
F.G. Suleman ◽  
J.-F. Ghersi-Egea ◽  
B. Leininger-Muller ◽  
A. Minn
Keyword(s):  
Rat Brain ◽  
Uridine Diphosphate ◽  
Uridine Diphosphate Glucuronosyltransferase ◽  
Brain Microsomes

Characterization of glutamate uptake systems in astrocyte primary cultures from rat brain

Glia ◽  
1991 ◽  
Vol 4 (3) ◽  
pp. 293-304 ◽  
Author(s):  
Berenike Flott ◽  
Wilfried Seifert
Keyword(s):  
Rat Brain ◽  
Glutamate Uptake ◽  
Primary Cultures

scholarly journals Molecular cloning of rat mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase and detection of the corresponding mRNA and of those encoding the remaining enzymes comprising the ketogenic 3-hydroxy-3-methylglutaryl-CoA cycle in central nervous system of suckling rat

1998 ◽  
Vol 329 (2) ◽  
pp. 373-381 ◽  
Author(s):  
E. Tim CULLINGFORD ◽  
T. Colin DOLPHIN ◽  
K. Kishore BHAKOO ◽  
Stefan PEUCHEN ◽  
Laura CANEVARI ◽  
...  
Keyword(s):  
Rat Brain ◽  
Ketone Bodies ◽  
Primary Cultures ◽  
Brain Regions ◽  
Preliminary Evidence ◽  
Direct Role ◽  
Rnase Protection ◽  
Mrna Species ◽  
Cortical Astrocyte ◽  
Degenerate Oligonucleotide

We have investigated, by RNase protection assays in rat brain regions and primary cortical astrocyte cultures, the presence of the mRNA species encoding the three mitochondrially located enzymes acetoacetyl-CoA thiolase, mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase (mt. HMG-CoA synthase) and HMG-CoA lyase (HMG-CoA lyase) that together constitute the ketogenic HMG-CoA cycle. As a prerequisite we obtained a full-length cDNA encoding rat HMG-CoA lyase by degenerate oligonucleotide-primed PCR coupled to a modification of PCR-rapid amplification of cDNA ends (PCR-RACE). We report here: (1) the nucleotide sequence of rat mt. HMG-CoA lyase, (2) detection of the mRNA species encoding all three HMG-CoA cycle enzymes in all regions of rat brain during suckling, (3) approximately twice the abundance of mt. HMG-CoA synthase mRNA in cerebellum than in cortex in 11-day-old suckling rat pups, (4) significantly lower abundances of mt. HMG-CoA synthase mRNA in brain regions derived from rats weaned to a high-carbohydrate/low-fat diet compared with the corresponding regions derived from the suckling rat, and (5) the presence of mt. HMG-CoA synthase mRNA in primary cultures of neonatal cortical astrocytes at an abundance similar to that found in liver of weaned animals. These results provide preliminary evidence that certain neural cell types possess ketogenic potential and might thus have a direct role in the provision of fatty acid-derived ketone bodies during the suckling period.

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