Hormonal Regulation of Aldolase B Gene Expression in Rat Primary Cultured Hepatocytes

1998 ◽  
Vol 350 (2) ◽  
pp. 291-297 ◽  
Author(s):  
Jun-itsu Ito ◽  
Takejiro Kuzumaki ◽  
Kaoru Otsu ◽  
Yoshihito Iuchi ◽  
Kiichi Ishikawa
Keyword(s):  
Gene Expression ◽  
Hormonal Regulation ◽  
Cultured Hepatocytes ◽  
Primary Cultured Hepatocytes ◽  
Aldolase B

scholarly journals PROFILING OF GENE EXPRESSION IN RAT LIVER AND RAT PRIMARY CULTURED HEPATOCYTES TREATED WITH PEROXISOME PROLIFERATORS

2006 ◽  
Vol 31 (5) ◽  
pp. 471-490 ◽  
Author(s):  
Kotaro TAMURA ◽  
Atsushi ONO ◽  
Toshikazu MIYAGISHIMA ◽  
Taku NAGAO ◽  
Tetsuro URUSHIDANI
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Peroxisome Proliferators ◽  
Cultured Hepatocytes ◽  
Primary Cultured Hepatocytes

scholarly journals Regulation of IGFBP-2 expression during fasting

2015 ◽  
Vol 467 (3) ◽  
pp. 453-460 ◽  
Author(s):  
Hye Suk Kang ◽  
Mi-Young Kim ◽  
Seung-Jae Kim ◽  
Jae-Ho Lee ◽  
Yong-Deuk Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Promoter ◽  
Biological Action ◽  
Peroxisome Proliferator ◽  
Cultured Hepatocytes ◽  
Peroxisome Proliferator Activated Receptor ◽  
Akt Phosphorylation ◽  
Primary Cultured Hepatocytes ◽  
Igf Binding ◽  
Responsive Element

Insulin-like growth factor (IGF)-binding protein-2 (IGFBP-2), one of the most abundant circulating IGFBPs, is known to attenuate the biological action of IGF-1. Although the effect of IGFBP-2 in preventing metabolic disorders is well known, its regulatory mechanism remains unclear. In the present study, we demonstrated the transcriptional regulation of the Igfbp-2 gene by peroxisome-proliferator-activated receptor (PPAR) α in the liver. During fasting, both Igfbp-2 and PPARα expression levels were increased. Wy14643, a selective PPARα agonist, significantly induced Igfbp-2 gene expression in primary cultured hepatocytes. However, Igfbp-2 gene expression in Pparα null mice was not affected by fasting or Wy14643. In addition, through transient transfection and chromatin immunoprecipitation assay in fasted livers, we determined that PPARα bound to the putative PPAR-responsive element between −511 bp and −499 bp on the Igfbp-2 gene promoter, indicating that the Igfbp-2 gene transcription is activated directly by PPARα. To explore the role of PPARα in IGF-1 signalling, we treated primary cultured hepatocytes with Wy14643 and observed a decrease in the number of IGF-1 receptors (IGF-1Rs) and in Akt phosphorylation. No inhibition was observed in the hepatocytes isolated from Pparα null mice. These results suggest that PPARα controls IGF-1 signalling through the up-regulation of hepatic Igfbp-2 transcription during fasting and Wy14643 treatment.

scholarly journals Amylin impairment of insulin effects on glycogen synthesis and phosphoenolpyruvate carboxykinase gene expression in rat primary cultured hepatocytes

1994 ◽  
Vol 304 (2) ◽  
pp. 449-453 ◽  
Author(s):  
S Baqué ◽  
J J Guinovart ◽  
A M Gómez-Foix
Keyword(s):  
Gene Expression ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Glycogen Synthesis ◽  
Mrna Level ◽  
Rat Hepatocytes ◽  
Synthesis Rate ◽  
Cultured Hepatocytes ◽  
Glycogen Accumulation ◽  
Primary Cultured Hepatocytes

The ability of amylin to impair hepatic insulin action is controversial. We have found that the effect of amylin in primary cultured hepatocytes is strongly dependent on the culture conditions. Only in hepatocytes preincubated in the presence of fetal serum did amylin, at concentrations ranging from 1 to 100 nM, reduce insulin-stimulated glycogen synthesis rate and glycogen accumulation without showing direct effects. Neither basal glycogen synthase nor glycogen phosphorylase activity was modified by amylin treatment. Nevertheless, amylin (100 nM) blocked the activation of glycogen synthase by insulin. Amylin also proved capable of opposing the reduction in the expression of the phosphoenolpyruvate carboxykinase (PEPCK) gene induced by insulin, whereas the basal mRNA level of PEPCK was unaffected by amylin treatment. Thus, these results show that, in cultured rat hepatocytes, amylin is indeed able to interfere with insulin regulation of glycogenesis and PEPCK gene expression, favouring the hypothesis that amylin may modulate liver sensitivity to insulin.

scholarly journals Dietary and hormonal regulation of aldolase B gene expression.

1985 ◽  
Vol 75 (3) ◽  
pp. 1045-1052 ◽  
Author(s):  
A Munnich ◽  
C Besmond ◽  
S Darquy ◽  
G Reach ◽  
S Vaulont ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hormonal Regulation ◽  
Aldolase B

Morphological and gene expression analysis in mouse primary cultured hepatocytes exposed to streptozotocin

2005 ◽  
Vol 56 (4-5) ◽  
pp. 245-253 ◽  
Author(s):  
Eisuke Kume ◽  
Chinami Aruga ◽  
Kaori Takahashi ◽  
Satoko Miwa ◽  
Eriha Dekura ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Cultured Hepatocytes ◽  
Primary Cultured Hepatocytes
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