Expression and Characterization of the Catalytic Domain of Human Phenylalanine Hydroxylase

1997 ◽  
Vol 348 (2) ◽  
pp. 295-302 ◽  
Author(s):  
S.Colette Daubner ◽  
Patrick J. Hillas ◽  
Paul F. Fitzpatrick
Keyword(s):  
Catalytic Domain ◽  
Phenylalanine Hydroxylase ◽  
Human Phenylalanine Hydroxylase

Structural characterization of the 5' regions of the human phenylalanine hydroxylase gene

Biochemistry ◽  
1992 ◽  
Vol 31 (35) ◽  
pp. 8363-8368 ◽  
Author(s):  
David S. Konecki ◽  
Yibin Wang ◽  
Friedrich K. Trefz ◽  
Uta Lichter-Konecki ◽  
Savio L. C. Woo
Keyword(s):  
Structural Characterization ◽  
Phenylalanine Hydroxylase ◽  
Hydroxylase Gene ◽  
Phenylalanine Hydroxylase Gene ◽  
Human Phenylalanine Hydroxylase

Crystal structure of the catalytic domain of human phenylalanine hydroxylase reveals the structural basis for phenylketonuria

1997 ◽  
Vol 4 (12) ◽  
pp. 995-1000 ◽  
Author(s):  
Heidi Erlandsen ◽  
Fabrizia Fusetti ◽  
Aurora Martinez ◽  
Edward Hough ◽  
Torgeir Flatmark ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Catalytic Domain ◽  
Phenylalanine Hydroxylase ◽  
Structural Basis ◽  
Human Phenylalanine Hydroxylase

scholarly journals Systematic Modification and Evaluation of Enzyme-Loaded Chitosan Nanoparticles

2021 ◽  
Vol 22 (15) ◽  
pp. 7987
Author(s):  
Paulo R. Lino ◽  
João Leandro ◽  
Lara Figueiredo ◽  
Mariana P. Amaro ◽  
Lídia M. D. Gonçalves ◽  
...  
Keyword(s):  
Protein Delivery ◽  
Phenylalanine Hydroxylase ◽  
Chitosan Nanoparticles ◽  
Delivery Systems ◽  
Diverse Range ◽  
Nano Drug Delivery ◽  
Human Phenylalanine Hydroxylase ◽  
Protein Instability ◽  
Nanoparticulate Systems

Polymeric-based nano drug delivery systems have been widely exploited to overcome protein instability during formulation. Presently, a diverse range of polymeric agents can be used, among which polysaccharides, such as chitosan (CS), hyaluronic acid (HA) and cyclodextrins (CDs), are included. Due to its unique biological and physicochemical properties, CS is one of the most used polysaccharides for development of protein delivery systems. However, CS has been described as potentially immunogenic. By envisaging a biosafe cytocompatible and haemocompatible profile, this paper reports the systematic development of a delivery system based on CS and derived with HA and CDs to nanoencapsulate the model human phenylalanine hydroxylase (hPAH) through ionotropic gelation with tripolyphosphate (TPP), while maintaining protein stability and enzyme activity. By merging the combined set of biopolymers, we were able to effectively entrap hPAH within CS nanoparticles with improvements in hPAH stability and the maintenance of functional activity, while simultaneously achieving strict control of the formulation process. Detailed characterization of the developed nanoparticulate systems showed that the lead formulations were internalized by hepatocytes (HepG2 cell line), did not reveal cell toxicity and presented a safe haemocompatible profile.

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