Allelic Variants of Human Cytochrome P450 2C9: Baculovirus-Mediated Expression, Purification, Structural Characterization, Substrate Stereoselectivity, and Prochiral Selectivity of the Wild-Type and I359L Mutant Forms

1996 ◽  
Vol 333 (2) ◽  
pp. 447-458 ◽  
Author(s):  
Robert L. Haining ◽  
Ann P. Hunter ◽  
Maurice E. Veronese ◽  
William F. Trager ◽  
Allan E. Rettie
Keyword(s):  
Cytochrome P450 ◽  
Structural Characterization ◽  
Wild Type ◽  
Cytochrome P450 2C9 ◽  
Allelic Variants ◽  
Human Cytochrome ◽  
Mutant Forms

Amino Acid Residues Affecting the Activities of Human Cytochrome P450 2C9 and 2C19

2002 ◽  
Vol 30 (8) ◽  
pp. 931-936 ◽  
Author(s):  
Toshiro Niwa ◽  
Akira Kageyama ◽  
Kae Kishimoto ◽  
Yoshiyasu Yabusaki ◽  
Fumihide Ishibashi ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Amino Acid ◽  
Amino Acid Residues ◽  
Cytochrome P450 2C9 ◽  
Human Cytochrome

Effects of Pomegranate Juice on Human Cytochrome P450 2C9 and Tolbutamide Pharmacokinetics in Rats

2006 ◽  
Vol 35 (2) ◽  
pp. 302-305 ◽  
Author(s):  
Masashi Nagata ◽  
Muneaki Hidaka ◽  
Hiroshi Sekiya ◽  
Yohei Kawano ◽  
Keishi Yamasaki ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Pomegranate Juice ◽  
Cytochrome P450 2C9 ◽  
Human Cytochrome ◽  
Pharmacokinetics In Rats

Catalytic activities of human cytochrome P450 2C9*1, 2C9*3 and 2C9*13

Xenobiotica ◽  
2005 ◽  
Vol 35 (9) ◽  
pp. 853-861 ◽  
Author(s):  
Y. Guo ◽  
Y. Wang ◽  
D. Si ◽  
P. J. Fawcett ◽  
D. Zhong ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 2C9 ◽  
Catalytic Activities ◽  
Human Cytochrome

scholarly journals Functional Characterization of Allelic Variants of Polymorphic Human Cytochrome P450 2A6 (CYP2A6*5, *7, *8, *18, *19, and *35)

2012 ◽  
Vol 35 (3) ◽  
pp. 394-399 ◽  
Author(s):  
Songhee Han ◽  
Seunghye Choi ◽  
Young-Jin Chun ◽  
Chul-Ho Yun ◽  
Chang Hoon Lee ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Functional Characterization ◽  
Allelic Variants ◽  
Human Cytochrome ◽  
Cytochrome P450 2A6

scholarly journals Potential Role for Human Cytochrome P450 3A4 in Estradiol Homeostasis

Endocrinology ◽  
2005 ◽  
Vol 146 (7) ◽  
pp. 2911-2919 ◽  
Author(s):  
Ai-Ming Yu ◽  
Katsumi Fukamachi ◽  
Kristopher W. Krausz ◽  
Connie Cheung ◽  
Frank J. Gonzalez
Keyword(s):  
Cytochrome P450 ◽  
Mammary Glands ◽  
Cytochrome P450 3A4 ◽  
Wild Type ◽  
Lactating Mothers ◽  
Human Cytochrome ◽  
Pup Survival ◽  
Cyp3a4 Inhibitors ◽  
Cyp3a4 Gene ◽  
Estradiol Levels

Abstract Previously, a human CYP3A4-transgenic (Tg-CYP3A4) mouse line was reported to exhibit enhanced metabolism of midazolam by cytochrome P450 3A4 (CYP3A4) expressed in small intestine. Here we show that expression of CYP3A4 and murine cyp3a and cyp2b was both age and sex dependent. CYP3A4 was expressed in the livers of male and female Tg-CYP3A4 mice at 2 and 4 wk of age. Since 6 wk, CYP3A4 was undetectable in male livers, whereas it was constitutively expressed in female livers at decreased levels (3- to 5-fold). Pregnenolone 16α-carbonitrile markedly induced hepatic CYP3A4 expression, and the level was higher in females than males. Induction of intrinsic murine cyp3a and cyp2b was also sex dependent. Tg-CYP3A4 females were found to be deficient in lactation, leading to a markedly lower pup survival. The mammary glands of the Tg-CYP3A4 lactating mothers had underdeveloped alveoli with low milk content. Furthermore, β-casein and whey acidic protein mRNAs were expressed at markedly lower levels in Tg-CYP3A4 pregnant and nursing mouse mammary glands compared with wild-type mice. This impaired lactation phenotype was associated with significantly reduced serum estradiol levels in Tg-CYP3A4 mice. A pharmacokinetic study revealed that the clearance of iv administrated [3H]estradiol was markedly enhanced in Tg-CYP3A4 mice compared with wild-type mice. These results suggest that CYP3A4 may play an important role in estradiol homeostasis. This may be of concern for treatment of pregnant and lactating women because CYP3A4 gene expression and enzymatic activity can be potentially modified by CYP3A4 inhibitors or inducers in medications, supplements, beverages, and diet.

New Insights into the Structural Features and Functional Relevance of Human Cytochrome P450 2C9. Part II

2009 ◽  
Vol 10 (10) ◽  
pp. 1127-1150 ◽  
Author(s):  
Sui-Lin Mo ◽  
Zhi-Wei Zhou ◽  
Li-Ping Yang ◽  
Ming Wei ◽  
Shu-Feng Zhou
Keyword(s):  
Cytochrome P450 ◽  
Structural Features ◽  
Cytochrome P450 2C9 ◽  
Human Cytochrome ◽  
Functional Relevance

scholarly journals Crystal structures of human cytochrome P450 2C9 and 3A4 with bound ligands

2004 ◽  
Vol 60 (a1) ◽  
pp. s140-s140
Author(s):  
D. Matak-Vinkovic ◽  
P. A. Williams ◽  
J. Cosme ◽  
A. Ward ◽  
P. Day ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Crystal Structures ◽  
Cytochrome P450 2C9 ◽  
Human Cytochrome
Sign in / Sign up

Export Citation Format

Share Document