Kinetic Characterization of Human Immunodeficiency Virus Type 1 Protease: Determination of Inhibitor Rate Constants during Dynamic Monomer–Dimer Interconversion

1996 ◽  
Vol 328 (2) ◽  
pp. 317-323 ◽  
Author(s):  
Maurice M. Morelock ◽  
Edward T. Graham ◽  
Donald Erdman ◽  
Christopher A. Pargellis
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Rate Constants ◽  
Kinetic Characterization ◽  
Immunodeficiency Virus

scholarly journals Kinetic Characterization of Human Immunodeficiency Virus Type-1 Protease-resistant Variants

1996 ◽  
Vol 271 (30) ◽  
pp. 17979-17985 ◽  
Author(s):  
S. Pazhanisamy ◽  
Cameron M. Stuver ◽  
Aine B. Cullinan ◽  
Nara Margolin ◽  
B. G. Rao ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Kinetic Characterization ◽  
Immunodeficiency Virus

Genotypic Characterization of Human Immunodeficiency Virus Type 1 Derived from Antiretroviral Therapy-Naive Individuals Residing in Sorong, West Papua

2016 ◽  
Vol 32 (8) ◽  
pp. 812-817 ◽  
Author(s):  
Adiana Mutamsari Witaningrum ◽  
Tomohiro Kotaki ◽  
Siti Qamariyah Khairunisa ◽  
Muhammad Qushai Yunifiar M ◽  
Dwi Wahyu Indriati ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Antiretroviral Therapy ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Genotypic Characterization ◽  
Immunodeficiency Virus ◽  
West Papua

scholarly journals Determination of Coreceptor Usage of Human Immunodeficiency Virus Type 1 from Patient Plasma Samples by Using a Recombinant Phenotypic Assay

2001 ◽  
Vol 75 (1) ◽  
pp. 251-259 ◽  
Author(s):  
Virginie Trouplin ◽  
Francesca Salvatori ◽  
Fanny Cappello ◽  
Veronique Obry ◽  
Anne Brelot ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Coreceptor Usage ◽  
Plasma Samples ◽  
Immunodeficiency Virus ◽  
Semiquantitative Evaluation ◽  
Convenient Technique

ABSTRACT We developed a recombinant virus technique to determine the coreceptor usage of human immunodeficiency virus type 1 (HIV-1) from plasma samples, the source expected to represent the most actively replicating virus population in infected subjects. This method is not subject to selective bias associated with virus isolation in culture, a step required for conventional tropism determination procedures. The addition of a simple subcloning step allowed semiquantitative evaluation of virus populations with a different coreceptor (CCR5 or CXCR4) usage specificity present in each plasma sample. This procedure detected mixtures of CCR5- and CXCR4-exclusive virus populations as well as dualtropic viral variants, in variable proportions. Sequence analysis of dualtropic clones indicated that changes in the V3 loop are necessary for the use of CXCR4 as a coreceptor, but the overall context of the V1-V3 region is important to preserve the capacity to use CCR5. This convenient technique can greatly assist the study of virus evolution and compartmentalization in infected individuals.

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