Cover: Zoo Biology, Volume 40 Issue 2 March/April 2021

Zoo Biology ◽  
2021 ◽  
Vol 40 (2) ◽  
Author(s):  
Gary W. Ferguson ◽  
William H. Gehrmann ◽  
Matthew S. Vaughan ◽  
Glenn C. Kroh ◽  
Deborah Chase ◽  
...  
Keyword(s):  
Zoo Biology

Forthcoming in zoo biology

Zoo Biology ◽  
1994 ◽  
Vol 13 (5) ◽  
pp. 525-525
Keyword(s):  
Zoo Biology

Forthcoming in Zoo Biology

Zoo Biology ◽  
2016 ◽  
Vol 35 (5) ◽  
pp. 463-463
Keyword(s):  
Zoo Biology

Empirical zoo: opportunities and challenges to a scientific zoo biology

Zoo Biology ◽  
2008 ◽  
Vol 27 (6) ◽  
pp. 431-435 ◽  
Author(s):  
Terry L. Maple
Keyword(s):  
Zoo Biology

276. The technical and biological validation of an LH assay for use with the Western Grey Kangaroo (Macropus fuliginosis) and Black-flanked Rock Wallaby (Petrogale lateralis lateralis)

2008 ◽  
Vol 20 (9) ◽  
pp. 76
Author(s):  
P. Matson ◽  
C. Mayberry ◽  
N. Willers ◽  
M. A. Blackberry ◽  
G. B. Martin
Keyword(s):  
Plasma Concentrations ◽  
Standard Curve ◽  
Biological Validation ◽  
Grey Kangaroo ◽  
Coefficients Of Variation ◽  
Western Grey Kangaroo ◽  
Technical Validation ◽  
Zoo Biology ◽  
Smithsonian Institute ◽  
And Control

Methods for the measurement of marsupial LH invariably rely upon the similarity of the LH molecule between different species and usually use anti-ovine or anti-bovine LH antibody and an ovine or bovine labelled LH preparation. Initial attempts to measure plasma LH in the Western Grey Kangaroo with assays using antibodies to 4 different isoforms of ovine LH raised in 7 different rabbits were unsuccessful. An enzymeimmunoassay (EIA) developed for the Asian elephant (Zoo Biology 23:45–63) was then applied to the Western Grey Kangaroo and the Black-flanked Rock Wallaby. This EIA has an anti-bovine-LH monoclonal antibody (518B7 provided by Dr Jan Roser, University of California, Davis, USA), biotinylated ovine LH label and bovine LH standard (NIADDK-oLH-26 and NIH-bLH-B10, both provided by Dr Janine Brown and Nicole Abbondanza, Smithsonian Institute, Front Royal, Virginia USA). Technical validation showed that serial dilution down to 1:8 of plasma from 7 individuals of each species showed parallelism to the assay standard curve, and control samples (1.24–5.30 ng/mL) had between-assay coefficients of variation <9%. Biological validation was achieved by challenging animals with intramuscular GnRH (Fertagyl®, 2.5 µg/kg) and measuring LH before and 25 min after the injection. Significant increases in plasma concentrations of LH (mean ± sem; all P > 0.0005) were seen after GnRH for both the Western Grey Kangaroo (from 5.0 ± 0.8 ng/mL to 9.4 ± 1.2 ng/mL; n = 19) and the Black-flanked Rock Wallaby (from 6.0 ± 0.7 ng/mL to 10.6 ± 0.6 ng/mL; n = 28). In conclusion, this assay can be successfully used to measure LH in these two species.

Forthcoming in zoo biology

Zoo Biology ◽  
1993 ◽  
Vol 12 (5) ◽  
pp. 508-508 ◽  
Keyword(s):  
Zoo Biology

The future of zoo biology

Zoo Biology ◽  
2008 ◽  
Vol 27 (6) ◽  
pp. 498-504 ◽  
Author(s):  
Dan Wharton
Keyword(s):  
The Future ◽  
Zoo Biology
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