A cell surface display system using novel GPI-anchored proteins inHansenula polymorpha

Yeast ◽  
2002 ◽  
Vol 19 (13) ◽  
pp. 1153-1163 ◽  
Author(s):  
So-Young Kim ◽  
Jung-Hoon Sohn ◽  
Yu-Ryang Pyun ◽  
Eui-Sung Choi
Keyword(s):  
Cell Surface ◽  
Surface Display ◽  
Cell Surface Display ◽  
Display System ◽  
A Cell ◽  
Cell Surface Display System

scholarly journals Development of a cell surface display system in Chlamydomonas reinhardtii

2022 ◽  
Vol 61 ◽  
pp. 102570
Author(s):  
João Vitor Dutra Molino ◽  
Roberta Carpine ◽  
Karl Gademann ◽  
Stephen Mayfield ◽  
Simon Sieber
Keyword(s):  
Cell Surface ◽  
Chlamydomonas Reinhardtii ◽  
Surface Display ◽  
Cell Surface Display ◽  
Display System ◽  
A Cell ◽  
Cell Surface Display System

scholarly journals Development of a Cell Surface Display System in a Magnetotactic Bacterium, “Magnetospirillum magneticum” AMB-1

2008 ◽  
Vol 74 (11) ◽  
pp. 3342-3348 ◽  
Author(s):  
Masayoshi Tanaka ◽  
Yuko Nakata ◽  
Tetsushi Mori ◽  
Yoshiko Okamura ◽  
Hitoshi Miyasaka ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Cell Surface ◽  
Magnetic Force ◽  
Secondary Structure Prediction ◽  
Surface Display ◽  
Cell Surface Display ◽  
Display System ◽  
A Cell ◽  
Magnetospirillum Magneticum ◽  
Cell Surface Display System

ABSTRACT Bacterial cell surface display is a widely used technology for bioadsorption and for the development of a variety of screening systems. Magnetotactic bacteria are unique species of bacteria due to the presence of magnetic nanoparticles within them. These intracellular, nanosized (50 to 100 nm) magnetic nanoparticles enable the cells to migrate and be manipulated by magnetic force. In this work, using this unique characteristic and based on whole-genomic and comprehensive proteomic analyses of these bacteria, a cell surface display system has been developed by expressing hexahistidine residues within the outer coiled loop of the membrane-specific protein (Msp1) of the “Magnetospirillum magneticum” (proposed name) AMB-1 bacterium. The optimal display site of the hexahistidine residues was successfully identified via secondary structure prediction, immunofluorescence microscopy, and heavy metal binding assay. The established AMB-1 transformant showed high immunofluorescence response, high Cd2+ binding, and high recovery efficiency in comparison to those of the negative control when manipulated by magnetic force.

scholarly journals Enhanced Display of Lipase on the Escherichia coli Cell Surface, Based on Transcriptome Analysis

2009 ◽  
Vol 76 (3) ◽  
pp. 971-973 ◽  
Author(s):  
Jong Hwan Baek ◽  
Mee-Jung Han ◽  
Seung Hwan Lee ◽  
Sang Yup Lee
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Transcriptome Analysis ◽  
Surface Display ◽  
Cell Surface Display ◽  
Display System ◽  
E Coli ◽  
A Cell ◽  
Anchoring Motif ◽  
Cell Surface Display System

ABSTRACT A cell surface display system was developed using Escherichia coli OmpC as an anchoring motif. The fused Pseudomonas fluorescens SIK W1 lipase was successfully displayed on the surface of E. coli cells, and the lipase activity could be enhanced by the coexpression of the gadBC genes identified by transcriptome analysis.

scholarly journals Construction of a food-grade cell surface display system for Lactobacillus casei

2014 ◽  
Vol 169 (9-10) ◽  
pp. 733-740 ◽  
Author(s):  
Jiayang Qin ◽  
Xiuwen Wang ◽  
Jian Kong ◽  
Cuiqing Ma ◽  
Ping Xu
Keyword(s):  
Cell Surface ◽  
Lactobacillus Casei ◽  
Surface Display ◽  
Cell Surface Display ◽  
Display System ◽  
Food Grade ◽  
Cell Surface Display System
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