Analysis of 41 kb of the DNA sequence from the right arm of chromosome II ofSchizosaccharomyces pombe

Yeast ◽  
2001 ◽  
Vol 18 (12) ◽  
pp. 1111-1116 ◽  
Author(s):  
Manuel Sánchez ◽  
José L. Revuelta ◽  
Francisco del Rey ◽  
Rhian Gwilliam ◽  
Jason Skelton ◽  
...  
Keyword(s):  
Yeast ◽  
1994 ◽  
Vol 10 (10) ◽  
pp. 1363-1381 ◽  
Author(s):  
Gertrud Mannhaupt ◽  
Rolf Stucka ◽  
Susanne Ehnle ◽  
Irene Vetter ◽  
Horst Feldmann

Yeast ◽  
1993 ◽  
Vol 9 (2) ◽  
pp. 189-199 ◽  
Author(s):  
Francois Doignon ◽  
Nicolas Biteau ◽  
Marc Crouzet ◽  
Michel Aigle

Yeast ◽  
1997 ◽  
Vol 13 (4) ◽  
pp. 357-363 ◽  
Author(s):  
JAVIER ARROYO ◽  
MELBA GARCÍA-GONZALEZ ◽  
M. ISABEL GARCÍA-SAEZ ◽  
MIGUEL SANCHEZ ◽  
CÉSAR NOMBELA

Yeast ◽  
1993 ◽  
Vol 9 (10) ◽  
pp. 1131-1137 ◽  
Author(s):  
Francois Doignon ◽  
Nicolas Biteau ◽  
Michel Aigle ◽  
Marc Crouzet

2003 ◽  
Vol 14 (6) ◽  
pp. 2461-2469 ◽  
Author(s):  
Tadayuki Shimada ◽  
Akira Yamashita ◽  
Masayuki Yamamoto

Fission yeast Mei2p is an RNA-binding protein essential for induction of both premeiotic DNA synthesis and first meiotic division. Mei2p forms a dot structure at an apparently fixed position in the horse-tail nucleus during meiotic prophase. This dot formation requires a meiosis-specific RNA species, meiRNA, which is indispensable for meiosis I, and the emergence of the dot is an indicator of the ability of the cell to perform meiosis I. Herein, we have sought the identity of this dot. Analyses using chromosome segregation in haploid meiosis, reciprocal translocation of chromosomes, and gene translocation have led us to conclude that the Mei2p dot is in association with the sme2 gene on the short arm of chromosome II, which encodes meiRNA. Transcripts of sme2, rather than the DNA sequence of the gene, seem to be the determinant of the localization of the Mei2p dot. However, evidence suggests that the dot may not be a simple reflection of the attachment of Mei2p to meiRNA undergoing transcription. We speculate that the Mei2p dot is a specialized structure, either to foster the assembly of Mei2p and meiRNA or to perform some unidentified function indispensable for meiosis I.


Author(s):  
Dieaa I. Nassr

Abstract We present a new version of the Secure Hash Algorithm-2 (SHA-2) formed on artificial sequences of deoxyribonucleic acid (DNA). This article is the first attempt to present the implementation of SHA-2 using DNA data processing. We called the new version DNSHA-2. We present new operations on an artificial DNA sequence, such as (1) $\bar {R}^{k}(\alpha)$ R ̄ k ( α ) and $\bar {L}^{k}(\alpha)$ L ̄ k ( α ) to mimic the right and left shift by k bits, respectively; (2) $\bar {S}^{k}(\alpha)$ S ̄ k ( α ) to mimic the right rotation by k bits; and (3) DNA-nucleotide addition (mod 264) to mimic word-wise addition (mod 264). We also show, in particular, how to carry out the different steps of SHA-512 on an artificial DNA sequence. At the same time, the proposed nucleotide operations can be used to mimic any hash algorithm of its bitwise operations similar to bitwise operations specified in SHA-2. The proposed hash has the following features: (1) it can be applied to all data, such as text, video, and image; (2) it has the same security level of SHA-2; and (3) it can be performed in a biological environment or on DNA computers.


Genetics ◽  
1992 ◽  
Vol 131 (2) ◽  
pp. 287-296 ◽  
Author(s):  
G Thon ◽  
A J Klar

Abstract The mat2-P and mat3-M loci of fission yeast contain respectively the plus (P) and minus (M) mating-type information in a transcriptionally silent state. That information is transposed from the mat2 or mat3 donor locus via recombination into the expressed mating-type locus (mat1) resulting in switching of the cellular mating type. We have identified a gene, named clr1 (for cryptic loci regulator), whose mutations allow expression of the mat2 and mat3 loci. clr1 mutants undergo aberrant haploid meiosis, indicative of transcription of the silent genes. Production of mRNA from mat3 is detectable in clr1 mutants. Furthermore, the ura4 gene inserted near mat3, weakly expressed in wild-type cells, is derepressed in clr1 mutants. The clr1 mutations also permit meiotic recombination in the 15-kb mat2-mat3 interval, where recombination is normally inhibited. The clr1 locus is in the right arm of chromosome II. We suggest that clr1 regulates silencing of the mat2 and mat3 loci, and participates in establishing the "cold spot" for recombination by organizing the chromatin structure of the mating-type region.


Yeast ◽  
1994 ◽  
Vol 10 (7) ◽  
pp. 959-964 ◽  
Author(s):  
Quirina J. M. Van Der Aart ◽  
H. Ydesteensma ◽  
Christophe Barthe ◽  
François Doignon ◽  
Michel Aigle ◽  
...  

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