scholarly journals How to characterize a strain? Clonal heterogeneity in industrial Saccharomyces influences both phenotypes and heterogeneity in phenotypes

Yeast ◽  
2021 ◽  
Author(s):  
Hanna Viktória Rácz ◽  
Fezan Mukhtar ◽  
Alexandra Imre ◽  
Zoltán Rádai ◽  
Andreas Károly Gombert ◽  
...  
Keyword(s):  
Clonal Heterogeneity

scholarly journals DNA Methylation and Intra-Clonal Heterogeneity: The Chronic Myeloid Leukemia Model

Cancers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 3587
Author(s):  
Benjamin Lebecque ◽  
Céline Bourgne ◽  
Véronique Vidal ◽  
Marc G. Berger
Keyword(s):  
Dna Methylation ◽  
Chronic Myeloid Leukemia ◽  
Fusion Protein ◽  
Myeloid Leukemia ◽  
Kinase Inhibitors ◽  
Single Gene ◽  
Clonal Heterogeneity ◽  
Sequencing Technologies ◽  
Genome Wide ◽  
The Impact

Chronic Myeloid Leukemia (CML) is a model to investigate the impact of tumor intra-clonal heterogeneity in personalized medicine. Indeed, tyrosine kinase inhibitors (TKIs) target the BCR-ABL fusion protein, which is considered the major CML driver. TKI use has highlighted the existence of intra-clonal heterogeneity, as indicated by the persistence of a minority subclone for several years despite the presence of the target fusion protein in all cells. Epigenetic modifications could partly explain this heterogeneity. This review summarizes the results of DNA methylation studies in CML. Next-generation sequencing technologies allowed for moving from single-gene to genome-wide analyses showing that methylation abnormalities are much more widespread in CML cells. These data showed that global hypomethylation is associated with hypermethylation of specific sites already at diagnosis in the early phase of CML. The BCR-ABL-independence of some methylation profile alterations and the recent demonstration of the initial intra-clonal DNA methylation heterogeneity suggests that some DNA methylation alterations may be biomarkers of TKI sensitivity/resistance and of disease progression risk. These results also open perspectives for understanding the epigenetic/genetic background of CML predisposition and for developing new therapeutic strategies.

DNA clonal heterogeneity of hepatocellular carcinoma demonstrated by Feulgen-DNA analysis

2008 ◽  
Vol 7 (6) ◽  
pp. 359-363 ◽  
Author(s):  
Sow-Hsong Kuo ◽  
Jin-Chuan Sheu ◽  
Ding-Shinn Chen ◽  
Juei-Low Sung ◽  
Chi-Chung Lin ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Analysis ◽  
Clonal Heterogeneity

scholarly journals High-capacity assay to quantify the clonal heterogeneity in potency of mesenchymal stem cells

2011 ◽  
Vol 5 (S8) ◽  
Author(s):  
Kim C O’Connor ◽  
Katie C Russell ◽  
Donald G Phinney ◽  
Michelle R Lacey ◽  
Bonnie L Barrilleaux ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
High Capacity ◽  
Clonal Heterogeneity

Clonal heterogeneity of fibroblasts from different human embryonic tissuesIn vitro

1984 ◽  
Vol 97 (5) ◽  
pp. 635-638
Author(s):  
S. M. Terekhov ◽  
Kh. A. Gatsadze ◽  
K. N. Grinberg
Keyword(s):  
Clonal Heterogeneity

Spatial genomics enables multi-modal study of clonal heterogeneity in tissues

Nature ◽  
2021 ◽  
Author(s):  
Tongtong Zhao ◽  
Zachary D. Chiang ◽  
Julia W. Morriss ◽  
Lindsay M. LaFave ◽  
Evan M. Murray ◽  
...  
Keyword(s):  
Clonal Heterogeneity

Clonal heterogeneity in the germinal zone of the developing rat telencephalon

Development ◽  
1993 ◽  
Vol 118 (1) ◽  
pp. 175-192 ◽  
Author(s):  
S.E. Acklin ◽  
D. van der Kooy
Keyword(s):  
Cell Cycle ◽  
Cell Lines ◽  
Spatial Organization ◽  
Precursor Cell ◽  
Clonal Heterogeneity ◽  
Double Labeling ◽  
Germinal Zone ◽  
Dividing Cells ◽  
Cellular Phenotypes ◽  
Developing Rat

A double-labeling technique, combining retroviral tagging of individual cell lines (one clone per brain hemisphere) with the simultaneous [3H]thymidine-labeling of dividing cells in S phase, was used to study proliferation characteristics of individual precursor cell lines in the germinal zone of the developing rat forebrain. The cortical germinal zone was found to be segregated into three spatially distinct horizontal populations of precursor cell lineages, which differed in cell cycle kinetics, amount of cell death, and synchronous versus asynchronous mode of proliferation. The striatal germinal zone demonstrated a similar heterogeneity in the cell cycle characteristics of proliferating clones, but did not show nearly as distinct a spatial segregation of these different populations. The results demonstrate the clonal heterogeneity among precursor populations in the telencephalon and the differential spatial organization of the cortical and the striatal germinal zones. This germinal zone heterogeneity may predict some of the differences found among cellular phenotypes in the adult forebrain.

Clonal heterogeneity in patients with cytogenetically normal acute myeloid leukemia withNPM1mutations

2012 ◽  
Vol 54 (5) ◽  
pp. 1056-1060 ◽  
Author(s):  
Dana Dvorakova ◽  
Zdenek Racil ◽  
Marek Borsky ◽  
Blanka Robesova ◽  
Ivana Jeziskova ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Clonal Heterogeneity ◽  
Acute Myeloid

In Vitro High-Capacity Assay to Quantify the Clonal Heterogeneity in Trilineage Potential of Mesenchymal Stem Cells Reveals a Complex Hierarchy of Lineage Commitment

Stem Cells ◽  
2010 ◽  
Vol 28 (4) ◽  
pp. 788-798 ◽  
Author(s):  
Katie C. Russell ◽  
Donald G. Phinney ◽  
Michelle R. Lacey ◽  
Bonnie L. Barrilleaux ◽  
Kristin E. Meyertholen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
High Capacity ◽  
Lineage Commitment ◽  
Clonal Heterogeneity
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