Construction and characterization of a zinc‐inducible gene expression vector in fission yeast

Yeast ◽  
2020 ◽  
Author(s):  
Shinya Takahata ◽  
Takahiro Asanuma ◽  
Miyuki Mori ◽  
Yota Murakami
Keyword(s):  
Gene Expression ◽  
Fission Yeast ◽  
Expression Vector ◽  
Inducible Gene Expression ◽  
Inducible Gene

scholarly journals Iterative experiment design guides the characterization of a light-inducible gene expression circuit

2015 ◽  
Vol 112 (26) ◽  
pp. 8148-8153 ◽  
Author(s):  
Jakob Ruess ◽  
Francesca Parise ◽  
Andreas Milias-Argeitis ◽  
Mustafa Khammash ◽  
John Lygeros
Keyword(s):  
Gene Expression ◽  
A Priori ◽  
Experiment Design ◽  
Design Flow ◽  
Biochemical Reaction Networks ◽  
Inducible Gene Expression ◽  
Optimal Experiment Design ◽  
Optimal Experiment ◽  
Inducible Gene

Systems biology rests on the idea that biological complexity can be better unraveled through the interplay of modeling and experimentation. However, the success of this approach depends critically on the informativeness of the chosen experiments, which is usually unknown a priori. Here, we propose a systematic scheme based on iterations of optimal experiment design, flow cytometry experiments, and Bayesian parameter inference to guide the discovery process in the case of stochastic biochemical reaction networks. To illustrate the benefit of our methodology, we apply it to the characterization of an engineered light-inducible gene expression circuit in yeast and compare the performance of the resulting model with models identified from nonoptimal experiments. In particular, we compare the parameter posterior distributions and the precision to which the outcome of future experiments can be predicted. Moreover, we illustrate how the identified stochastic model can be used to determine light induction patterns that make either the average amount of protein or the variability in a population of cells follow a desired profile. Our results show that optimal experiment design allows one to derive models that are accurate enough to precisely predict and regulate the protein expression in heterogeneous cell populations over extended periods of time.

scholarly journals Development and Characterization of a Xylose-Inducible Gene Expression System for Clostridium perfringens

2011 ◽  
Vol 77 (23) ◽  
pp. 8439-8441 ◽  
Author(s):  
Hirofumi Nariya ◽  
Shigeru Miyata ◽  
Tomomi Kuwahara ◽  
Akinobu Okabe
Keyword(s):  
Gene Expression ◽  
Clostridium Perfringens ◽  
Expression System ◽  
Xylose Isomerase ◽  
Inducible Gene Expression ◽  
Content Type ◽  
Regulated Expression ◽  
Operator Sequence ◽  
Inducible Gene

ABSTRACTA xylose-inducible gene expression vector forClostridium perfringenswas developed. Plasmid pXCH contains a chromosomal region fromClostridium difficile(xylR-PxylB):xylR, encoding the xylose repressor,xylO, thexyloperator sequence, and PxylB, the divergent promoter upstream ofxylBAencoding xylulo kinase and xylose isomerase. pXCH allows tightly regulated expression of the chloramphenicol acetyltransferase reporter and the α-toxin genes in response to the inducer concentration. Thus, pXCH could constitute a new valuable genetic tool for study ofC. perfringens.

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