Simultaneous visualisation of the complete sets of telomeres from the Mme I generated terminal restriction fragments in yeasts

Yeast ◽  
2020 ◽  
Vol 37 (11) ◽  
pp. 585-595
Author(s):  
Jun Liu ◽  
Xiaojing Hong ◽  
Chao‐Ya Liang ◽  
Jun‐Ping Liu
Science ◽  
1983 ◽  
Vol 222 (4619) ◽  
pp. 72-74 ◽  
Author(s):  
H. Erlich ◽  
D Stetler ◽  
R Sheng-Dong ◽  
D Ness ◽  
C Grumet

2005 ◽  
Vol 49 (5) ◽  
pp. 1957-1964 ◽  
Author(s):  
Susanna K. P. Lau ◽  
Pak-leung Ho ◽  
Maria W. S. Li ◽  
Hoi-wah Tsoi ◽  
Raymond W. H. Yung ◽  
...  

ABSTRACT Laribacter hongkongensis, a newly discovered bacterium recently shown to be associated with community-acquired gastroenteritis, is generally resistant to most β-lactams except the carbapenems. We describe the cloning and characterization of a novel chromosomal class C β-lactamase and its regulatory gene in L. hongkongensis. Two genes, ampC and ampR, were cloned by inserting restriction fragments of genomic DNA from L. hongkongensis strain HLHK5 into pBK-CMV to give the recombinant plasmid pBK-LHK-5. The ampR and ampC genes and their promoters were divergently oriented, with the ampR gene immediately upstream of the ampC gene and an intercistronic Lys-R motif, typical of inducible ampC-ampR regulatory systems. The deduced amino acid sequence of the cloned AmpC β-lactamase (pI 8.1) contained consensus motifs characteristic of class C β-lactamases but had identities no greater than 46% to known class C β-lactamases. The kinetic properties of this AmpC were also compatible with those of a class C β-lactamase. PCR of 20 clinical isolates of L. hongkongensis, including HLHK5, showed the presence of both ampC and ampR genes in all isolates. Southern hybridization suggested that the ampC gene of HLHK5 was chromosomally encoded. Subcloning experiments showed that the expression of the ampC gene of HLHK5 was regulated by its ampR gene, which acts as a repressor. The β-lactamase characterized from strain HLHK5 was named LHK-5 (gene, bla LHK-5) and represents the first example of AmpC β-lactamase in the β subdivision of proteobacteria.


2021 ◽  
Vol 2021 (5) ◽  
Author(s):  
Francisca Carrillo-Morales ◽  
Francisco Correa ◽  
Olaf Lechtenfeld

Abstract For the rational quantum Calogero systems of type A1⊕A2, AD3 and BC3, we explicitly present complete sets of independent conserved charges and their nonlinear algebras. Using intertwining (or shift) operators, we include the extra ‘odd’ charges appearing for integral couplings. Formulæ for the energy eigenstates are used to tabulate the low-level wave functions.


2013 ◽  
Vol 46 (48) ◽  
pp. 485303 ◽  
Author(s):  
Jukka Kiukas ◽  
Jussi Schultz
Keyword(s):  

1974 ◽  
Vol 15 (10) ◽  
pp. 1787-1799 ◽  
Author(s):  
B. R. Judd ◽  
W. Miller ◽  
J. Patera ◽  
P. Winternitz

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