Melatonin improves survival and respiratory activity of yeast cells challenged by alpha-synuclein and menadione

Yeast ◽  
2017 ◽  
Vol 35 (3) ◽  
pp. 281-290 ◽  
Author(s):  
Mariana A. Zampol ◽  
Mario H. Barros
Keyword(s):  
Respiratory Activity ◽  
Alpha Synuclein ◽  
Yeast Cells

scholarly journals RELATION OF OXYGEN TENSION AND TEMPERATURE TO THE TIME OF REDUCTION OF CYTOCHROME

1935 ◽  
Vol 19 (2) ◽  
pp. 339-350 ◽  
Author(s):  
T. J. B. Stier
Keyword(s):  
Absorption Band ◽  
Linear Relation ◽  
Optical Method ◽  
Respiratory Activity ◽  
Yeast Cells ◽  
O2 Consumption ◽  
Average Difference ◽  
Absorption Bands ◽  
Experimental Conditions ◽  
Reduction Time

The time for the appearance of the cytochrome C absorption band after shaking a suspension of bakers' yeast with various O2-N2 mixtures was determined at each of six temperatures. At each temperature a linear relation between this interval—called the reduction time—and O2 tension was found. It was shown: 1. That under our experimental conditions, absorption bands of cytochrome were seen when the O2 tension of the suspension was reduced to, or below, a certain pressure which was found to be specific for each temperature (this pressure is provisionally considered to be identical with or very near to the "critical O2 tension" usually found in QOO2-O2-tension relationships); 2. That the x-axis intercept obtained from the reduction time - O2-tension plot gives the value of the "critical" O2 pressure at each temperature; 3. That the O2 tension within the suspension is reduced by the respiratory activity of the yeast cells. An equation describing these observations is given and is used in calculating rates of O2 consumption from measurements of reduction time of cytochrome. The average difference between the calculated values and the manometric measurements of QOO2 was found to be 6.6 per cent. A rapid optical method of measuring rates of O2 consumption based on the findings of these experiments is proposed for use with cytochrome-containing microorganisms.

scholarly journals An Illuminated Respiratory Activity Monitoring System Identifies Priming-Active Compounds in Plant Seedlings

2021 ◽  
Author(s):  
Judith Loogen ◽  
André Müller ◽  
Arne Balzer ◽  
Sophie Weber ◽  
Kathrin Schmitz ◽  
...  
Keyword(s):  
Monitoring System ◽  
Respiratory Activity ◽  
Oxygen Transfer Rate ◽  
Positive Control ◽  
Activity Monitoring ◽  
Yeast Cells ◽  
Respiration Activity ◽  
Active Compounds ◽  
Amino Acid Peptide ◽  
Plant Seedlings

Abstract Background Growing large crop monocultures and heavily using pesticides enhances the chance of evolution of pesticide-insensitive pests and pathogens. To reduce pesticide use in crop cultivation, the application of priming-active compounds (PrimACs) is a welcome alternative. PrimACs strengthen the plant immune system and can thus help to protect plants with lower amounts of pesticides. PrimACs can be identified, for example, by their capacity to enhance the respiratory activity of parsley cells in culture as determined by the oxygen transfer rate (OTR) using the respiration activity monitoring system (RAMOS) or its miniaturized version, µRAMOS. The latter was designed for with suspensions of bacteria and yeast cells in microtiter plates (MTPs). So far, RAMOS or µRAMOS have not been applied to adult plants or seedlings which would overcome the limitation of (µ)RAMOS to plant suspension cell cultures. Results In this work, we introduce a modified µRAMOS for analysis of plant seedlings. The novel device allows illuminating the seedlings and records the respiratory activity in each well of a 48-well MTP. To validate the suitability of the setup for identifying novel PrimAC in Arabidopsis thaliana, seedlings were grown in MTP for seven days and treated with the known PrimAC salicylic acid (SA; positive control) and the PrimAC candidate methyl 1-(3,4-dihydroxyphenyl)-2-oxocyclopentane-1-carboxylate (Tyr020). Twenty-eight h after treatment, the seedlings were elicited with flg22, a 22-amino acid peptide of bacterial flagellin. Upon elicitation, the respiratory activity was monitored. The evaluation of the OTR course reveals Tyr020 as a likely PrimAC. The priming-inducing activity of Tyr020 was confirmed using molecular biological analyses in A. thaliana seedlings. Conclusion We disclose the suitability of µRAMOS for identifying PrimACs in plant seedlings. The difference in OTR during a night period between primed and unprimed plants was clearly distinguishable after elicitation with flg22. Thus, it has been shown, that the µRAMOS device can be used for a reliable screening for PrimACs in plant seedlings.

scholarly journals Construction of model strain of yeast Saccharomyces cerevisiae with regulated expression of recombinant human alpha-synuclein

2021 ◽  
Vol 15 (3) ◽  
pp. 41-50
Author(s):  
N. V. Hrushanyk ◽  
◽  
Y. I. Fedorko ◽  
O. V. Stasyk ◽  
O. G. Stasyk ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Green Fluorescent Protein ◽  
Recombinant Protein ◽  
Recombinant Strain ◽  
Lipid Membrane ◽  
Fluorescent Protein ◽  
Intracellular Localization ◽  
Alpha Synuclein ◽  
Yeast Cells ◽  
Green Fluorescent

Background. Improper folding and accumulation of a-synuclein aggregates are among the causes of Parkinson’s disease. The most important factor influencing the process of α-synuclein aggregation is the level of this protein in neurons which depends on the balance between its synthesis, degradation and secretion. Under certain conditions, when α-synuclein is synthesized at a high level, monomers of this protein can aggregate on the lipid membrane, which leads to the formation of amyloids, fibrils and protofibrils unable to perform their physiological functions. Since it is virtually impossible to study the properties of α-synuclein in vivo, researchers are actively using model biological systems (single-celled microorganisms, human cell lines, animal models etc.). The aim of this study was to construct a recombinant strain of Saccharomyces cerevisiae with controlled expression of human α-synuclein to study the regulation and properties of this protein and for screening for new low molecular weight chemi­cal compounds which can induce α-synuclein aggregation and/or degradation. Materials and methods. A recombinant strain of S. cerevisiae with controlled expression of α-synuclein conjugated to a green fluorescent protein was isolated. Western blotting with specific anti-α-synuclein antibodies was used to detect recombinant α-synuclein in yeast cells. Intracellular localization of heterologous chimeric green fluorescent protein conjugated to α-synuclein was also examined by fluorescence microscopy. Results. To construct a recombinant strain of S. cerevisiae, the coding sequence of the human wild-type α-synuclein gene was expressed under the regulated promoter of the ScMET25 gene. Analysis of the effect of different concentrations of exogenous methionine as a factor regulating the expression of the ScMET25 promoter on the content of recombinant protein showed that the expression of the human α-synuclein gene in S. cerevisiae is repressed in the presence of methionine at a concentration of 10 mg/L and higher. During long-term cultivation of yeast cells, this effect decreased due to the depletion of methionine in the growth medium. As a result, recombinant protein synthesis was restored, and α-synuclein content in such cells approached that of cells grown in a medium with a low concentration of (5 mg/L), or without methionine. It was also found that overproduction of recombinant α-synuclein in S. cerevisiae cells had virtually no effect on culture growth, indicating the absence or a very weak toxic effect of human α-synuclein on yeast physiology. Conclusions. The obtained data indicate a concentration-dependent effect of methionine on the level of recombinant α-synuclein synthesis in S. cerevisiae yeast cells. Such controlled expression of the studied protein can be used to screen for compounds capable of promoting dose-dependent aggregation or degradation of α-synuclein in yeast cells and potentially in human cells as well.

scholarly journals An illuminated respiratory activity monitoring system identifies priming-active compounds in plant seedlings

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Judith Loogen ◽  
André Müller ◽  
Arne Balzer ◽  
Sophie Weber ◽  
Kathrin Schmitz ◽  
...  
Keyword(s):  
Monitoring System ◽  
Respiratory Activity ◽  
Oxygen Transfer Rate ◽  
Positive Control ◽  
Activity Monitoring ◽  
Yeast Cells ◽  
Respiration Activity ◽  
Active Compounds ◽  
Amino Acid Peptide ◽  
Plant Seedlings

Abstract Background Growing large crop monocultures and heavily using pesticides enhances the evolution of pesticide-insensitive pests and pathogens. To reduce pesticide use in crop cultivation, the application of priming-active compounds (PrimACs) is a welcome alternative. PrimACs strengthen the plant immune system and could thus help to protect plants with lower amounts of pesticides. PrimACs can be identified, for example, by their capacity to enhance the respiratory activity of parsley cells in culture as determined by the oxygen transfer rate (OTR) using the respiration activity monitoring system (RAMOS) or its miniaturized version, µRAMOS. The latter was designed for with suspensions of bacteria and yeast cells in microtiter plates (MTPs). So far, RAMOS or µRAMOS have not been applied to adult plants or seedlings, which would overcome the limitation of (µ)RAMOS to plant suspension cell cultures. Results In this work, we introduce a modified µRAMOS for analysis of plant seedlings. The novel device allows illuminating the seedlings and records the respiratory activity in each well of a 48-well MTP. To validate the suitability of the setup for identifying novel PrimAC in Arabidopsis thaliana, seedlings were grown in MTP for seven days and treated with the known PrimAC salicylic acid (SA; positive control) and the PrimAC candidate methyl 1-(3,4-dihydroxyphenyl)-2-oxocyclopentane-1-carboxylate (Tyr020). Twenty-eight h after treatment, the seedlings were elicited with flg22, a 22-amino acid peptide of bacterial flagellin. Upon elicitation, the respiratory activity was monitored. The evaluation of the OTR course reveals Tyr020 as a likely PrimAC. The priming-inducing activity of Tyr020 was confirmed using molecular biological analyses in A. thaliana seedlings. Conclusion We disclose the suitability of µRAMOS for identifying PrimACs in plant seedlings. The difference in OTR during a night period between primed and unprimed plants was distinguishable after elicitation with flg22. Thus, it has been shown that the µRAMOS device can be used for a reliable screening for PrimACs in plant seedlings.

Bioenergetics of Yarrowia lipolytica Cells Grown at Alkaline Conditions

2004 ◽  
Vol 24 (2) ◽  
pp. 117-125 ◽  
Author(s):  
Renata A. Zvyagilskaya ◽  
Olga A. Parkhomenko ◽  
Anna V. Gordeeva ◽  
Yuliya I. Deryabina ◽  
Bengt L. Persson
Keyword(s):  
Oxidative Phosphorylation ◽  
Yarrowia Lipolytica ◽  
Respiratory Activity ◽  
Respiratory Control ◽  
Yeast Cells ◽  
The Novel ◽  
Energy Status ◽  
Oxidative Phosphorylation System ◽  
Alkaline Conditions ◽  
Tightly Coupled

Energy status of the novel alkalitolerant Yarrowia lipolytica yeast strain grown at alkaline conditions (pH 9.7) was examined. Cells grown under such severe conditions were found to preserve high respiratory activity. The oxidative phosphorylation system dominated in the energy budget of the cell. A procedure was specially design to isolate tightly coupled mitochondria from yeast cells grown at alkaline conditions. The isolated mitochondrial preparations met known criteria of physiological intactness, as inferred from their ability to maintain distinctive state 4–3 respiration transition upon addition of ADP, high respiratory rates, good respiratory control values, and ADP/O ratios close to the theoretically expected maxima for the substrates used.

Evaluation of the Inhibiting Effects from Exposure to Microwaves on the Respiratory Activity of Yeast Cells or on Enzyme Activity

2003 ◽  
Vol 10 (8) ◽  
pp. 663-669 ◽  
Author(s):  
L. Campanella ◽  
M. Cusano ◽  
R. Dragone ◽  
M. Sammartino ◽  
G. Visco
Keyword(s):  
Enzyme Activity ◽  
Respiratory Activity ◽  
Yeast Cells

scholarly journals Caloric restriction rescues yeast cells from alpha-synuclein toxicity through autophagic control of proteostasis

Aging ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 3821-3833 ◽  
Author(s):  
Belém Sampaio-Marques ◽  
Hélder Pereira ◽  
Ana R. Santos ◽  
Alexandra Teixeira ◽  
Paula Ludovico
Keyword(s):  
Caloric Restriction ◽  
Alpha Synuclein ◽  
Yeast Cells
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