Saccharomyces cerevisiae mRNA 3′ end forming signals are also involved in transcription termination

Yeast ◽  
1995 ◽  
Vol 11 (5) ◽  
pp. 447-453 ◽  
Author(s):  
Patrick Russo
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Termination

scholarly journals Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae.

1984 ◽  
Vol 4 (8) ◽  
pp. 1515-1520 ◽  
Author(s):  
S Henikoff ◽  
E H Cohen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drosophila Melanogaster ◽  
Signal Sequence ◽  
Transcription Termination ◽  
Gene Segment ◽  
Deletion Analysis ◽  
Base Pairs ◽  
Yeast Genes

We have mapped a signal sequence for mRNA 3'-end formation in Saccharomyces cerevisiae by using a Drosophila melanogaster DNA segment that complements a yeast adenine-8 mutation. That the 3' end of the transcript in S. cerevisiae nearly coincides with that in D. melanogaster is consistent with the possibility that mRNA termini are similarly determined in both organisms. Deletion analysis reveals that the complete signal is no more than 21 base pairs long. Part of the signal is the sequence TTTTTATA, which is seen in the termination region of several yeast genes. TTTTTATA appears to be able to act autonomously as a partial termination signal. The efficiency of the complete signal is affected by substitution of sequences downstream from it. This modulation of the effect of a signal is consistent with termination in S. cerevisiae, resembling rho-dependent termination in bacteria.

scholarly journals Transcriptomes of six mutants in the Sen1 pathway reveal combinatorial control of transcription termination across the Saccharomyces cerevisiae genome

PLoS Genetics ◽  
2017 ◽  
Vol 13 (6) ◽  
pp. e1006863 ◽  
Author(s):  
Xin Chen ◽  
Kunal Poorey ◽  
Melissa N. Carver ◽  
Ulrika Müller ◽  
Stefan Bekiranov ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Termination ◽  
Combinatorial Control

scholarly journals Nrd1p identifies aberrant and natural exosomal target messages during the nuclear mRNA surveillance in Saccharomyces cerevisiae

2020 ◽  
Author(s):  
Pragyan Singh ◽  
Anusha Chaudhuri ◽  
Mayukh Banerjea ◽  
Neeraja Marathe ◽  
Biswadip Das
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rna Polymerase Ii ◽  
Transcription Termination ◽  
Transcription Elongation ◽  
Late Phase ◽  
Vital Role ◽  
Mrna Surveillance ◽  
Normal Functional ◽  
Nuclear Exosome ◽  
Mrnp Biogenesis

AbstractIn all eukaryotes, selective nuclear degradation of aberrant mRNAs by nuclear exosome and its cofactors TRAMP, and CTEXT contribute to the fidelity of the gene expression pipeline. In the model eukaryote, Saccharomyces cerevisiae, the Nrd1p-Nab3p-Sen1p (NNS) complex, previously known to be involved in the transcription termination and matured 3’-end formation of vast majority of non-coding and several coding RNAs, is demonstrated to universally participate in the nuclear decay of various kinds of faulty messages in this study. Consistently, nrd1-1/nrd1-2 mutant cells display impairment of the decay of all kinds of aberrant mRNAs, like the yeast mutants deficient in Rrp41p, Rrp6p, and Rrp4p. nrd1ΔCID mutation (consisting of Nrd1p lacking its CID domain thereby abrogating its interaction with RNAPII) however, abolishes the decay of aberrant messages generated during early phases of mRNP biogenesis (transcription elongation, splicing and 3’-end maturation) without affecting the decay rate of the export-defective mRNAs. Mutation in the 3’-end processing factor, Pcf11p, in contrast, displayed a selective abolition of the decay of the aberrant mRNAs, generated at the late phase of mRNP biogenesis (export-defective mRNAs) without influencing the faulty messages spawned in the early phase of mRNP biogenesis. Co-transcriptional recruitment of Nrd1p onto the faulty messages, which relies on RNAPII during transcription elongation and on Pcf11p post transcription, is vital for the exosomal decay of aberrant mRNAs, as Nrd1p deposition on the export-defective messages led to the Rrp6p recruitment and eventually, their decay. Thus, presence of the ‘Nrd1p mark’ on aberrant mRNAs appears rate-limiting for the distinction of the aberrant messages from their normal functional counterparts.Author’s SummaryAberrant/faulty mRNAs generated from the deficiencies in any of the mRNP biogenesis events are promptly eliminated by the nuclear exosome and its cofactors TRAMP and CTEXT complexes. These machineries work relentlessly in the nucleus to detect all kinds of aberrant mRNAs and selectively target them for destruction. However, initial detection of a minuscule amount of aberrant mRNA in the vast background of normal mRNAs is quite challenging and its mechanism remains elusive. In this work, we demonstrate that, the trimeric Nrd1p-Nab3p-Sen1p complex, previously implicated in the transcription termination of diverse non-coding RNAs and a handful of mRNAs, constitute an integral component of the nuclear mRNA surveillance mechanism in baker’s yeast Saccharomyces cerevisiae. Major component of this complex, Nrd1p is demonstrated to be recruited selectively onto various classes of representative model aberrant messages either co-transcriptionally by RNA Polymerase II or post-transcriptionally by Pcf11p. Binding of Nrd1p to the export-defective special mRNAs further leads to the recruitment of Rrp6p on to them thereby leading to their degradation. NNS complex thus plays a vital role of initially recognizing the faulty messages and further assists in the recruitment of the nuclear exosome for their prompt elimination.

Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae

1984 ◽  
Vol 4 (8) ◽  
pp. 1515-1520 ◽  
Author(s):  
S Henikoff ◽  
E H Cohen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drosophila Melanogaster ◽  
Signal Sequence ◽  
Transcription Termination ◽  
Gene Segment ◽  
Deletion Analysis ◽  
Base Pairs ◽  
Yeast Genes

We have mapped a signal sequence for mRNA 3'-end formation in Saccharomyces cerevisiae by using a Drosophila melanogaster DNA segment that complements a yeast adenine-8 mutation. That the 3' end of the transcript in S. cerevisiae nearly coincides with that in D. melanogaster is consistent with the possibility that mRNA termini are similarly determined in both organisms. Deletion analysis reveals that the complete signal is no more than 21 base pairs long. Part of the signal is the sequence TTTTTATA, which is seen in the termination region of several yeast genes. TTTTTATA appears to be able to act autonomously as a partial termination signal. The efficiency of the complete signal is affected by substitution of sequences downstream from it. This modulation of the effect of a signal is consistent with termination in S. cerevisiae, resembling rho-dependent termination in bacteria.

scholarly journals A Mutation in GRS1, a Glycyl-tRNA Synthetase, Affects 3′-End Formation in Saccharomyces cerevisiae

Genetics ◽  
1999 ◽  
Vol 152 (1) ◽  
pp. 129-141
Author(s):  
Christi Magrath ◽  
Linda E Hyman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mutant Strain ◽  
Transcription Termination ◽  
Trna Synthetase ◽  
Single Amino Acid ◽  
Temperature Sensitive ◽  
Termination Factor ◽  
Transcription Termination Factor ◽  
Single Amino Acid Change ◽  
Yeast Genes

Abstract 3′-end formation is a complex and incompletely understood process involving both cis-acting and trans-acting factors. As part of an effort to examine the mechanisms of transcription termination by RNA polymerase II, a mutant hunt for strains defective in 3′-end formation was conducted. Following random mutagenesis, a temperature-sensitive strain exhibiting several phenotypes consistent with a role in transcription termination was isolated. First, readthrough of a terminator increases significantly in the mutant strain. Accordingly, RNA analysis indicates a decrease in the level of terminated transcripts, both in vivo and in vitro. Moreover, a plasmid stability assay in which high levels of readthrough lead to high levels of plasmid loss and transcription run-on analysis also demonstrate defective termination of transcription. Examination of polyadenylation and cleavage by the mutant strain indicates these processes are not affected. These results represent the first example of a transcription termination factor in Saccharomyces cerevisiae that affects transcription termination independent of 3′-end processing of mRNA. Complementation studies identified GRS1, an aminoacyl-tRNA synthetase, as the complementing gene. Sequence analysis of grs1-1 in the mutant strain revealed that nucleotides 1656 and 1657 were both C to T transitions, resulting in a single amino acid change of proline to phenylalanine. Further studies revealed GRS1 is essential, and the grs1-1 allele confers the temperature-sensitive growth defect associated with the mutant strain. Finally, we observed structures with some similarity to tRNA molecules within the 3′-end of various yeast genes. On the basis of our results, we suggest Grs1p is a transcription termination factor that may interact with the 3′-end of pre-mRNA to promote 3′-end formation.

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