Role of O-acetylhomoserine sulfhydrylase in sulfur amino acid synthesis in various yeasts

Yeast ◽  
1993 ◽  
Vol 9 (12) ◽  
pp. 1335-1342 ◽  
Author(s):  
Jerzy Brzywczy ◽  
Andrzej Paszewski
Keyword(s):  
Amino Acid ◽  
Acid Synthesis ◽  
Sulfur Amino Acid ◽  
Amino Acid Synthesis

scholarly journals Role of the DksA-Like Protein in the Pathogenesis and Diverse Metabolic Activity of Campylobacter jejuni

2008 ◽  
Vol 190 (13) ◽  
pp. 4512-4520 ◽  
Author(s):  
Jiae Yun ◽  
Byeonghwa Jeon ◽  
Yi-Wen Barton ◽  
Paul Plummer ◽  
Qijing Zhang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Campylobacter Jejuni ◽  
Pathogenic Bacteria ◽  
Acid Metabolism ◽  
Acid Synthesis ◽  
Regulatory Role ◽  
Intestinal Cells ◽  
Amino Acid Synthesis ◽  
Metabolic Reactions

ABSTRACT DksA is well known for its regulatory role in the transcription of rRNA and genes involved in amino acid synthesis in many bacteria. DksA has also been reported to control expression of virulence genes in pathogenic bacteria. Here, we elucidated the roles of a DksA-like protein (CJJ81176_0160, Cj0125c) in the pathogenesis of Campylobacter jejuni. As in other bacteria, transcription of stable RNA was repressed by the DksA-like protein under stress conditions in C. jejuni. Transcriptomic and proteomic analyses of C. jejuni 81-176 and an isogenic mutant lacking the DksA-like protein showed differential expression of many genes involved in amino acid metabolism, iron-related metabolism, and other metabolic reactions. Also, the C. jejuni DksA-like protein mutant exhibited a decreased ability to invade intestinal cells and induce release of interleukin-8 from intestinal cells. These results suggest that the DksA-like protein plays an important regulatory role in diverse metabolic events and the virulence of C. jejuni.

scholarly journals The Role of the Tricarboxylic Acid Cycle in Amino Acid Synthesis in Escherichia Coli

1953 ◽  
Vol 39 (10) ◽  
pp. 1013-1019 ◽  
Author(s):  
R. B. Roberts ◽  
D. B. Cowie ◽  
R. Britten ◽  
E. Bolton ◽  
P. H. Abelson
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Tricarboxylic Acid Cycle ◽  
Acid Synthesis ◽  
Tricarboxylic Acid ◽  
Amino Acid Synthesis ◽  
Acid Cycle

Roles and regulation of the glutamate racemase isogenes, racE and yrpC, in Bacillus subtilis

Microbiology ◽  
2004 ◽  
Vol 150 (9) ◽  
pp. 2911-2920 ◽  
Author(s):  
Keitarou Kimura ◽  
Lam-Son Phan Tran ◽  
Yoshifumi Itoh
Keyword(s):  
Bacillus Subtilis ◽  
Amino Acid ◽  
Minimal Medium ◽  
Degradation Products ◽  
Acid Synthesis ◽  
Rich Medium ◽  
Amino Acid Synthesis ◽  
Double Mutation ◽  
Glutamate Racemase

Many bacteria, including Escherichia coli, have a unique gene that encodes glutamate racemase. This enzyme catalyses the formation of d-glutamate, which is necessary for cell wall peptidoglycan synthesis. However, Bacillus subtilis has two glutamate racemase genes, named racE and yrpC. Since racE appears to be indispensable for growth in rich medium, the role of yrpC in d-amino acid synthesis is vague. Experiments with racE- and yrpC-knockout mutants confirmed that racE is essential for growth in rich medium but showed that this gene was dispensable for growth in minimal medium, where yrpC executes the anaplerotic role of racE. LacZ fusion assays demonstrated that racE was expressed in both types of media but yrpC was expressed only in minimal medium, which accounted for the absence of yrpC function in rich medium. Neither racE nor yrpC was required for B. subtilis cells to synthesize poly-γ-dl-glutamate (γ-PGA), a capsule polypeptide of d- and l-glutamate linked through a γ-carboxylamide bond. Wild-type cells degraded the capsule during the late stationary phase without accumulating the degradation products, d-glutamate and l-glutamate, in the medium. In contrast, racE or yrpC mutant cells accumulated significant amounts of d- but not l-glutamate. Exogenous d-glutamate utilization was somewhat defective in the mutants and the double mutation of race and yrpc severely impaired d-amino acid utilization. Thus, both racemase genes appear necessary to complete the catabolism of exogenous d-glutamate generated from γ-PGA.

scholarly journals Petasis vs. Strecker Amino Acid Synthesis: Convergence, Divergence and Opportunities in Organic Synthesis

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1707
Author(s):  
Wayiza Masamba
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Organic Synthesis ◽  
Acid Synthesis ◽  
Physical Sciences ◽  
Amino Acid Synthesis

α-Amino acids find widespread applications in various areas of life and physical sciences. Their syntheses are carried out by a multitude of protocols, of which Petasis and Strecker reactions have emerged as the most straightforward and most widely used. Both reactions are three-component reactions using the same starting materials, except the nucleophilic species. The differences and similarities between these two important reactions are highlighted in this review.

scholarly journals Enzymatic cascade systems for D-amino acid synthesis: progress and perspectives

2021 ◽  
Author(s):  
Anwen Fan ◽  
Jiarui Li ◽  
Yangqing Yu ◽  
Danping Zhang ◽  
Yao Nie ◽  
...  
Keyword(s):  
Amino Acid ◽  
Acid Synthesis ◽  
Amino Acid Synthesis ◽  
Cascade Systems

Reconstitution of amino acid synthesis by combining spinach chloroplasts with other leaf organelles

1979 ◽  
Vol 18 (7) ◽  
pp. 1109-1111 ◽  
Author(s):  
Barbara Buchholz ◽  
Brigitte Reupke ◽  
Horst Bickel ◽  
Gernot Schultz
Keyword(s):  
Amino Acid ◽  
Acid Synthesis ◽  
Amino Acid Synthesis ◽  
Spinach Chloroplasts

Enzymes for amino acid synthesis by design

2021 ◽  
Vol 4 (5) ◽  
pp. 348-349
Author(s):  
Christopher K. Prier
Keyword(s):  
Amino Acid ◽  
Acid Synthesis ◽  
Amino Acid Synthesis
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