Cloning and genetic characterization of a calcium- and phospholipid-binding protein fromSaccharomyces cerevisiae that is homologous to translation elongation factor-1γ

Yeast ◽  
1993 ◽  
Vol 9 (2) ◽  
pp. 151-163 ◽  
Author(s):  
Nicholas G. Kambouris ◽  
Daniel J. Burke ◽  
Carl E. Creutz
Keyword(s):  
Genetic Characterization ◽  
Binding Protein ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Phospholipid Binding

scholarly journals In vivo characterization of the role of tissue‐specific translation elongation factor 1 A 2 in protein synthesis reveals insights into muscle atrophy

FEBS Journal ◽  
2013 ◽  
Vol 280 (24) ◽  
pp. 6528-6540 ◽  
Author(s):  
Jennifer Doig ◽  
Lowri A. Griffiths ◽  
David Peberdy ◽  
Permphan Dharmasaroja ◽  
Maria Vera ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Muscle Atrophy ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
In Vivo Characterization ◽  
Elongation Factor 1

Identification of a Novel Binding Protein of FAT10: Eukaryotic Translation Elongation Factor 1A1

2012 ◽  
Vol 57 (9) ◽  
pp. 2347-2354 ◽  
Author(s):  
Xin Yu ◽  
Xiuxia Liu ◽  
Tiande Liu ◽  
Kui Hong ◽  
Jun Lei ◽  
...  
Keyword(s):  
Binding Protein ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Eukaryotic Translation

scholarly journals Characterization of the translation elongation factor 1-α gene in a wide range of pathogenic Aspergillus species

2017 ◽  
Vol 66 (4) ◽  
pp. 419-429 ◽  
Author(s):  
Sadegh Nouripour-Sisakht ◽  
Bahram Ahmadi ◽  
Koichi Makimura ◽  
Sybren de Hoog ◽  
Yoshiko Umeda ◽  
...  
Keyword(s):  
Elongation Factor ◽  
Aspergillus Species ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Wide Range ◽  
Elongation Factor 1

scholarly journals Isolation and characterization of monoclonal antibodies against eukaryotic translation elongation factor 1A

2003 ◽  
Vol 19 (3) ◽  
pp. 231-237
Author(s):  
A. P. Pogrebnaya ◽  
N. V. Markeyeva ◽  
V. V. Lisogubov ◽  
A. M. Fridberg ◽  
S. I. Zabashnyi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Eukaryotic Translation ◽  
Isolation And Characterization ◽  
Translation Elongation Factor 1A

Molecular Characterization of the cDNA Coding for Translation Elongation Factor-2 of Pathogenic Entamoeba histolytica

1993 ◽  
Vol 12 (1) ◽  
pp. 89-96 ◽  
Author(s):  
BARBARA PLAIMAUER ◽  
STEPHAN ORTNER ◽  
GERHARD WIEDERMANN ◽  
OTTO SCHEINER ◽  
MICHAEL DUCHÊNE
Keyword(s):  
Molecular Characterization ◽  
Entamoeba Histolytica ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 2

scholarly journals Structural Basis for EarP-Mediated Arginine Glycosylation of Translation Elongation Factor EF-P

mBio ◽  
2017 ◽  
Vol 8 (5) ◽  
Author(s):  
Ralph Krafczyk ◽  
Jakub Macošek ◽  
Pravin Kumar Ankush Jagtap ◽  
Daniel Gast ◽  
Swetlana Wunder ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Structural Model ◽  
Biochemical Characterization ◽  
Elongation Factor ◽  
Structural Basis ◽  
Inhibitor Design ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Enzymatic Mechanism

ABSTRACT Glycosylation is a universal strategy to posttranslationally modify proteins. The recently discovered arginine rhamnosylation activates the polyproline-specific bacterial translation elongation factor EF-P. EF-P is rhamnosylated on arginine 32 by the glycosyltransferase EarP. However, the enzymatic mechanism remains elusive. In the present study, we solved the crystal structure of EarP from Pseudomonas putida. The enzyme is composed of two opposing domains with Rossmann folds, thus constituting a B pattern-type glycosyltransferase (GT-B). While dTDP-β-l-rhamnose is located within a highly conserved pocket of the C-domain, EarP recognizes the KOW-like N-domain of EF-P. Based on our data, we propose a structural model for arginine glycosylation by EarP. As EarP is essential for pathogenicity in P. aeruginosa, our study provides the basis for targeted inhibitor design. IMPORTANCE The structural and biochemical characterization of the EF-P-specific rhamnosyltransferase EarP not only provides the first molecular insights into arginine glycosylation but also lays the basis for targeted-inhibitor design against Pseudomonas aeruginosa infection. IMPORTANCE The structural and biochemical characterization of the EF-P-specific rhamnosyltransferase EarP not only provides the first molecular insights into arginine glycosylation but also lays the basis for targeted-inhibitor design against Pseudomonas aeruginosa infection.

scholarly journals Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China

MycoKeys ◽  
2021 ◽  
Vol 80 ◽  
pp. 77-90
Author(s):  
Xianhong Wang ◽  
Yashuang Guo ◽  
Yamin Du ◽  
Ziling Yang ◽  
Xinzhong Huang ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Wide Range ◽  
Elongation Factor 1

Species of Diaporthe infect a wide range of plants and live in vivo as endophytes, saprobes or pathogens. However, those in peach plants are poorly characterized. In this study, 52 Diaporthe strains were isolated from peach branches with buds, showing constriction canker symptoms. Phylogenetic analyses were conducted using five gene regions: internal transcribed spacer of the ribosomal DNA (ITS), translation elongation factor 1-α (TEF), ß-tubulin (TUB), histone (HIS), and calmodulin (CAL). These results coupled with morphology revealed seven species of Diaporthe, including five known species (D. caryae, D. cercidis, D. eres, D. hongkongensis, and D. unshiuensis). In addition, two novel species D. jinxiu and D. zaofenghuang are introduced. Except for the previously reported D. eres, this study represents the first characterization of Diaporthe species associated with peach constriction canker in China, and contributes useful data for practicable disease management.

scholarly journals Characterization of physical properties of two isoforms of translation elongation factor 1A

2007 ◽  
Vol 23 (5) ◽  
pp. 386-390 ◽  
Author(s):  
A. V. Novosylna ◽  
A. A. Timchenko ◽  
E. I. Tiktopulo ◽  
I. N. Serdyuk ◽  
B. S. Negrutskii ◽  
...  
Keyword(s):  
Physical Properties ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Translation Elongation Factor 1A
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