Yeast sequencing reports. LEU2 gene homolog inKluyveromyces lactis

Yeast ◽  
1992 ◽  
Vol 8 (9) ◽  
pp. 801-804 ◽  
Author(s):  
Ying-Pei Zhang ◽  
Xin-Jie Chen ◽  
Yu-Yang Li ◽  
Hiroshi Fukuhara
Keyword(s):  
Leu2 Gene

Site-specific mutagenesis of cdc2+, a cell cycle control gene of the fission yeast Schizosaccharomyces pombe

1986 ◽  
Vol 6 (10) ◽  
pp. 3523-3530
Author(s):  
R Booher ◽  
D Beach
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Cell Cycle Control ◽  
Temperature Sensitive ◽  
Protein Kinase Activity ◽  
Atp Binding Site ◽  
Leu2 Gene ◽  
Cdc2 Gene ◽  
A Site

The cdc2+ gene of Schizosaccharomyces pombe is homologous to the CDC28 gene of Saccharomyces cerevisiae. Both genes share limited homology with vertebrate protein kinases and have protein kinase activity. cdc2+ has been subjected to mutagenesis in vitro. A null allele of the gene, constructed by insertion of the S. cerevisiae LEU2 gene into a site within the gene, has a phenotype similar to that of many temperature-sensitive alleles of cdc2. Mutations within the predicted ATP-binding site and in a region which may be a site of phosphorylation result in loss of cdc2+ activity. A single substitution of Gly-146 to Asp-146 has been identified in cdc2-1w, a dominant activated allele of the gene. The four introns within the cdc2+ gene have been deleted. The resulting gene not only functions in fission yeast but also rescues cdc28(Ts) strains of S. cerevisiae, a property which is not shared by the genomic cdc2+ gene.

Restoration of the yeast LEU2 gene by transcriptionally controlled recombination between tandem repeats

1990 ◽  
Vol 223 (1) ◽  
pp. 107-113 ◽  
Author(s):  
Mátyás Mink ◽  
A. Nazli Basak ◽  
Hans Küntzel
Keyword(s):  
Tandem Repeats ◽  
Leu2 Gene

Cloning of the LEU2 gene from the amylolytic yeast Saccharomycopsis fibuligera

2015 ◽  
Vol 24 (6) ◽  
pp. 2151-2154
Author(s):  
Eun-Hee Park ◽  
Soo-Hwan Yeo ◽  
Myoung-Dong Kim
Keyword(s):  
Saccharomycopsis Fibuligera ◽  
Leu2 Gene

A Short Region from the LEU2 Gene of Saccharomyces cerevisiae Functions as an ARS in the Yeast Saccharomyces exiguus Yp74L-3

1998 ◽  
Vol 37 (6) ◽  
pp. 426-430 ◽  
Author(s):  
Taisuke Hisatomi ◽  
Yutaka Wada ◽  
Chiaki Fujisaki ◽  
Michio Tsuboi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Short Region ◽  
Leu2 Gene ◽  
Saccharomyces Exiguus

Nucleotide sequence of the 3' terminal region of the LEU2 gene from Saccharomyces cerevisiae

Gene ◽  
1984 ◽  
Vol 31 (1-3) ◽  
pp. 257-261 ◽  
Author(s):  
Byron E. Froman ◽  
Robert C. Tait ◽  
Raymond L. Rodriguez
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleotide Sequence ◽  
Terminal Region ◽  
Leu2 Gene

scholarly journals Genetic mapping of Ty elements in Saccharomyces cerevisiae.

1984 ◽  
Vol 4 (2) ◽  
pp. 329-339 ◽  
Author(s):  
H L Klein ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Statistical Analysis ◽  
Genetic Mapping ◽  
Transposable Element ◽  
Genetic Map ◽  
Selectable Marker ◽  
Repeated Element ◽  
Ty Elements ◽  
Reciprocal Exchange ◽  
Leu2 Gene

We used transformation to insert a selectable marker at various sites in the Saccharomyces cerevisiae genome occupied by the transposable element Ty. The vector CV9 contains the LEU2+ gene and a portion of the repeated element Ty1-17. Transformation with this plasmid resulted in integration of the vector via a reciprocal exchange using homology at the LEU2 locus or at the various Ty elements that are dispersed throughout the S. cerevisiae genome. These transformants were used to map genetically sites of several Ty elements. The 24 transformants recovered at Ty sites define 19 distinct loci. Seven of these were placed on the genetic map. Two classes of Ty elements were identified in these experiments: a Ty1-17 class and Ty elements different from Ty1-17. Statistical analysis of the number of transformants at each class of Ty elements shows that there is preferential integration of the CV9 plasmid into the Ty1-17 class.

Extramitochondrial citrate synthase activity in bakers' yeast

1986 ◽  
Vol 6 (2) ◽  
pp. 488-493
Author(s):  
T M Rickey ◽  
A S Lewin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Citrate Synthase ◽  
Glucose Repression ◽  
Yeast Cells ◽  
Mitochondrial Enzyme ◽  
Wild Type ◽  
Reading Frame ◽  
Leu2 Gene ◽  
Growth Requirements ◽  
Wild Type Yeast

We isolated the gene for citrate synthase (citrate oxaloacetate lyase; EC 4.1.3.7) from Saccharomyces cerevisiae and ablated it by inserting the yeast LEU2 gene within its reading frame. This revealed a second, nonmitochondrial citrate synthase. Like the mitochondrial enzyme, this enzyme was sensitive to glucose repression. It did not react with antibodies against mitochondrial citrate synthase. Haploid cells lacking a gene for mitochondrial citrate synthase grew somewhat slower than wild-type yeast cells, but exhibited no auxotrophic growth requirements.

Use of interplasmid recombination to generate stable selectable markers for yeast transformation: application to studies of actin gene control

Genome ◽  
1990 ◽  
Vol 33 (5) ◽  
pp. 696-706 ◽  
Author(s):  
Andrew V. Hubberstey ◽  
Alan G. Wildeman
Keyword(s):  
Blot Analysis ◽  
Actin Gene ◽  
Multicopy Plasmid ◽  
Recombinant Plasmids ◽  
Leu2 Gene ◽  
Recombination System ◽  
Plasmid Recombination ◽  
Intact Gene ◽  
Multiple Copies ◽  
2 Μm Plasmid

A plasmid recombination system has been developed that relies upon interplasmid exchanges for yeast cell viability. Two types of plasmids, one carrying the LEU2 allele inserted within yeast actin gene sequences and the other carrying 2-μm plasmid DNA and an intact actin gene, were constructed. Neither plasmid alone yielded transformants in the haploid Leu− strain AH22, but when cotransformed, a number of colonies were obtained. Southern blot analysis revealed that transformants arose because of recombination events within the homologous actin sequences that transferred the LEU2 gene to the actin gene on the 2-μm plasmid. The recombinant plasmids could be recovered, and sequence analysis of one recombination site revealed that the exchange event was faithful at the nucleotide level. The resulting recombinant plasmids carried a defective actin gene and presumably arose because of a double-crossover event. Deletion mutations that prevented actin gene expression on one donor plasmid enabled the recovery at a high frequency of transformants resulting primarily from single-crossover events between the two plasmids. This was presumably because such events no longer generated an intact actin gene on a multicopy plasmid. Infrequently a transformant from a plasmid with an intact gene was recovered, but in these cases the plasmid was not present in multiple copies. These cells exhibited a slower growth rate, and Northern blot analysis revealed an elevated level of actin mRNA.Key words: homologous recombination, regulation, gene disruption, tranformation, actin.

Cloning of the LEU2 gene of Saccharomyces cerevisiae by in vivo recombination

1989 ◽  
Vol 152 (3) ◽  
pp. 263-268 ◽  
Author(s):  
R. Valinger ◽  
G. Braus ◽  
P. Niederberger ◽  
M. K�nzler ◽  
G. Paravicini ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Leu2 Gene ◽  
In Vivo Recombination
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