Lipid production from hemicellulose withLipomyces starkeyiin a pH regulated fed-batch cultivation

Yeast ◽  
2016 ◽  
Vol 33 (8) ◽  
pp. 451-462 ◽  
Author(s):  
Jule Brandenburg ◽  
Johanna Blomqvist ◽  
Jana Pickova ◽  
Nemailla Bonturi ◽  
Mats Sandgren ◽  
...  
Keyword(s):  
Lipid Production ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Fed Batch Cultivation

scholarly journals Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

2020 ◽  
Vol 8 (2) ◽  
pp. 151 ◽  
Author(s):  
Poontawee ◽  
Limtong
Keyword(s):  
Crude Glycerol ◽  
Lipid Production ◽  
Cell Mass ◽  
Batch Cultivation ◽  
Feeding Strategies ◽  
Fed Batch ◽  
Two Stage ◽  
Microbial Lipid Production ◽  
Constant Feeding ◽  
Fed Batch Cultivation

Microbial lipids are able to produce from various raw materials including lignocellulosic biomass by the effective oleaginous microorganisms using different cultivation processes. This study aimed to enhance microbial lipid production from the low-cost substrates namely sugarcane top hydrolysate and crude glycerol by Rhodosporidiobolus fluvialis DMKU-SP314, using two-stage fed-batch cultivation with different feeding strategies in a 3 L stirred-tank fermenter. The effect of two feeding strategies of 147.5 g/L crude glycerol solution was evaluated including pulse feeding at different starting time points (48, 24, and 72 h after initiation of batch operation) and constant feeding at different dilution rates (0.012, 0.020, and 0.033 h−1). The maximum lipid concentration of 23.6 g/L and cell mass of 38.5 g/L were achieved when constant feeding was performed at the dilution rate of 0.012 h−1 after 48 h of batch operation, which represented 1.24-fold and 1.27-fold improvements in the lipid and cell mass concentration, respectively. Whereas, batch cultivation provided 19.1 g/L of lipids and 30.3 g/L of cell mass. The overall lipid productivity increased to 98.4 mg/L/d in the two-stage fed-batch cultivation. This demonstrated that the two-stage fed-batch cultivation with constant feeding strategy has the possibility to apply for large-scale production of lipids by yeast.

scholarly journals Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 62
Author(s):  
Konstantins Dubencovs ◽  
Janis Liepins ◽  
Arturs Suleiko ◽  
Anastasija Suleiko ◽  
Reinis Vangravs ◽  
...  
Keyword(s):  
Kluyveromyces Marxianus ◽  
Reaction Medium ◽  
Value Added ◽  
Batch Cultivation ◽  
Batch Processes ◽  
Biomass Growth ◽  
High Cell ◽  
Media Composition ◽  
Fed Batch ◽  
Fed Batch Cultivation

The Kluyveromyces marxianus yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how to achieve high cell densities. However, depending on the physiology of the particular microbial strain, an optimal media composition should be used to avoid by-product synthesis and, subsequently, a decrease in overall process effi-ciency. Thus, the aim of the present study was to optimise the synthetic growth medium and feeding solution compositions (in terms of carbon, nitrogen, phosphorous, magnesium, and calcium concentrations) for high cell density K. marxianus fed‑batch cultivations. Additionally, the biomass yields from the vitamin mixture and other macro/microelements were identified. A model predictive control algorithm was successfully applied for a fed-batch cultivation control. Biomass growth and substrate consumption kinetics were compared with the mathematical model predictions. Finally, 2‑phenylethanol biosynthesis was induced and its productivity was estimated. The determined optimal macronutrient ratio for K. marxianus biomass growth was identified as C:N:P = 1:0.07:0.011. The maximal attained yeast biomass concentration was close to 70 g·L-1 and the 2-PE biosynthesis rate was 0.372 g·L−1·h−1, with a yield of 74% from 2-phenylalanine.

scholarly journals Xylitol production by Candida parapsilosis under fed-batch culture

2001 ◽  
Vol 44 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Sandra A. Furlan ◽  
Heizir F. de Castro
Keyword(s):  
Candida Parapsilosis ◽  
Batch Cultivation ◽  
Xylitol Production ◽  
Fed Batch ◽  
Substrate Consumption ◽  
Feed Medium ◽  
Fed Batch Culture ◽  
Rates Of Growth ◽  
Fed Batch Cultivation ◽  
Single Batch

Xylitol production by Candida parapsilosis was investigated under fed-batch cultivation, using single (xylose) or mixed (xylose and glucose) sugars as substrates. The presence of glucose in the medium induced the production of ethanol as secondary metabolite and improved specific rates of growth, xylitol formation and substrate consumption. Fractionated supply of the feed medium at constant sugar concentration did not promote any increase on the productivity compared to the single batch cultivation.

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