scholarly journals Purification of G1daughter cells from differentSaccharomycetesspecies through an optimized centrifugal elutriation procedure

Yeast ◽  
2014 ◽  
Vol 31 (5) ◽  
pp. 159-166 ◽  
Author(s):  
Martial Marbouty ◽  
Caroline Ermont ◽  
Bernard Dujon ◽  
Guy-Franck Richard ◽  
Romain Koszul
Keyword(s):  
Centrifugal Elutriation

Synchronization of 9L rat brain tumor cells by centrifugal elutriation

1980 ◽  
Vol 2 (3) ◽  
pp. 191-206 ◽  
Author(s):  
P. C. Keng ◽  
C. K. N. Li ◽  
K. T. Wheeler
Keyword(s):  
Brain Tumor ◽  
Rat Brain ◽  
Tumor Cells ◽  
Centrifugal Elutriation ◽  
Brain Tumor Cells ◽  
Rat Brain Tumor

Large-scale cell separation by centrifugal elutriation

1992 ◽  
Vol 200 (2) ◽  
pp. 376-380 ◽  
Author(s):  
M.P. Lutz ◽  
G. Gaedicke ◽  
W. Hartmann
Keyword(s):  
Cell Separation ◽  
Large Scale ◽  
Centrifugal Elutriation

scholarly journals Thymic regulation of hematopoiesis. III: Isolation of helper and suppressor populations using counterflow centrifugal elutriation

Blood ◽  
1986 ◽  
Vol 68 (3) ◽  
pp. 787-789
Author(s):  
SJ Sharkis ◽  
C Cremo ◽  
MI Collector ◽  
SJ Noga ◽  
AD Donnenberg
Keyword(s):  
Cell Types ◽  
Colony Growth ◽  
Minority Population ◽  
Intermediate Cell ◽  
Centrifugal Elutriation ◽  
Hematopoietic Progenitors ◽  
Majority Population ◽  
Helper Function ◽  
Positive And Negative Feedback

We have evidence that thymic regulatory cells can either enhance or inhibit the growth of hematopoietic progenitors in vitro. We have suggested that two separate populations are responsible for this regulatory interaction but isolation of the cell types has proven difficult. We now report the isolation by counterflow centrifugal elutriation (CCE) of two separate populations of thymocytes which regulate erythropoiesis in coculture. We demonstrate that a minority population (less than 10%) of slow sedimenting elutriated thymocytes provide a helper function whereas the suppressor population is the majority population. Furthermore, some thymocytes of intermediate cell volume neither enhance nor inhibit erythroid colony growth. We conclude that isolation of thymic subsets can lead to identification of populations which induce cell-cell regulation of hematopoietic progenitors resulting in both a positive and negative feedback control of growth.

scholarly journals Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction

2002 ◽  
Vol 2 ◽  
pp. 1643-1645 ◽  
Author(s):  
John Graham
Keyword(s):  
Gastric Mucosa ◽  
Ion Transport ◽  
Parietal Cell ◽  
Cell Function ◽  
Parietal Cells ◽  
Centrifugal Elutriation ◽  
Ecl Cells ◽  
Acid Secreting ◽  
Density Barrier

Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport and the endocrine/paracrine ECL cells effectively control parietal cell function. Discontinuous gradients of iodixanol for the purification of ECL cells were subsequently simplified to the use of a density barrier. This technique is now commonly used following initial centrifugal elutriation.

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