The high-osmolarity glycerol- and cell wall integrity-MAP kinase pathways ofSaccharomyces cerevisiaeare involved in adaptation to the action of killer toxin HM-1

Yeast ◽  
2012 ◽  
Vol 29 (11) ◽  
pp. 475-485 ◽  
Author(s):  
Masahiko Miyamoto ◽  
Yasuhiro Furuichi ◽  
Tadazumi Komiyama
Keyword(s):  
Cell Wall ◽  
Map Kinase ◽  
Killer Toxin ◽  
Cell Wall Integrity ◽  
High Osmolarity ◽  
High Osmolarity Glycerol ◽  
Map Kinase Pathways

scholarly journals A network of MAP-Kinase pathways and transcription factors regulates cell-to-cell communication and cell wall integrity in Neurospora crassa

2018 ◽  
Author(s):  
Monika S. Fischer ◽  
Vincent W. Wu ◽  
Ji E. Lee ◽  
Ronan C. O’Malley ◽  
N. Louise Glass
Keyword(s):  
Cell Wall ◽  
Transcription Factors ◽  
Map Kinase ◽  
Neurospora Crassa ◽  
Cell Fusion ◽  
Regulatory Network ◽  
Cell Communication ◽  
Cell Wall Integrity ◽  
Cell Integrity ◽  
Map Kinase Pathways

ABSTRACTMaintenance of cell integrity and cell-to-cell communication are fundamental biological processes. Filamentous fungi, such as Neurospora crassa, depend on communication to locate compatible cells, coordinate cell fusion, and establish a robust hyphal network. Two MAP-Kinase pathways are essential for communication and cell fusion in N. crassa; the Cell Wall Integrity/MAK-1 pathway and the MAK-2 (signal response) pathway. Previous studies have demonstrated several points of cross talk between the MAK-1 and MAK-2 pathways, which is likely necessary for oordinating chemotropic growth toward an extracellular signal, and then mediating cell fusion. Canonical MAP-Kinase pathways begin with signal reception and end with a transcriptional response. Two transcription factors, ADV-1 and PP-1, are essential for communication and cell fusion. PP-1 is the conserved target of MAK-2, while it is unclear what targets ADV-1. We did RNAseq on Δadv-1, Δpp-1, and wild-type cells and found that ADV-1 and PP-1 have a shared regulon including many genes required for communication, cell fusion, growth, development, and stress response. We identified ADV-1 and PP-1 binding sites across the genome by adapting the in vitro method of DNA-Affinity Purification sequencing (DAP-seq) for N. crassa. To elucidate the regulatory network, we misexpressed each transcription factor in each upstream MAPK deletion mutant. Misexpression of adv-1 was sufficient to fully suppress the phenotype of the Δpp-1 mutant and partially suppress the phenotype of the Δmak-1 mutant. Collectively, our data demonstrate that the MAK-1-ADV-1 and MAK-2- PP-1 pathways form a tight regulatory network that maintains cell integrity and mediates communication and cell fusion.

The high-osmolarity glycerol (HOG) and cell wall integrity (CWI) signalling pathways interplay: a yeast dialogue between MAPK routes

Yeast ◽  
2010 ◽  
Vol 27 (8) ◽  
pp. 495-502 ◽  
Author(s):  
Jose Manuel Rodríguez-Peña ◽  
Raúl García ◽  
César Nombela ◽  
Javier Arroyo
Keyword(s):  
Cell Wall ◽  
Cell Wall Integrity ◽  
Signalling Pathways ◽  
High Osmolarity ◽  
High Osmolarity Glycerol

scholarly journals Roles of High Osmolarity Glycerol and Cell Wall Integrity Pathways in Cadmium Toxicity in Saccharomyces cerevisiae

2021 ◽  
Vol 22 (12) ◽  
pp. 6169
Author(s):  
Yunying Zhao ◽  
Shiyun Li ◽  
Jing Wang ◽  
Yingli Liu ◽  
Yu Deng
Keyword(s):  
Cell Death ◽  
Cell Wall ◽  
Cadmium Toxicity ◽  
Cell Wall Integrity ◽  
Yeast Cells ◽  
Nuclear Accumulation ◽  
Hog Pathway ◽  
High Osmolarity ◽  
High Osmolarity Glycerol ◽  
Upr Pathway

Cadmium is a carcinogen that can induce ER stress, DNA damage, oxidative stress and cell death. The yeast mitogen-activated protein kinase (MAPK) signalling pathways paly crucial roles in response to various stresses. Here, we demonstrate that the unfolded protein response (UPR) pathway, the high osmolarity glycerol (HOG) pathway and the cell wall integrity (CWI) pathway are all essential for yeast cells to defend against the cadmium-induced toxicity, including the elevated ROS and cell death levels induced by cadmium. We show that the UPR pathway is required for the cadmium-induced phosphorylation of HOG_MAPK Hog1 but not for CWI_MAPK Slt2, while Slt2 but not Hog1 is required for the activation of the UPR pathway through the transcription factors of Swi6 and Rlm1. Moreover, deletion of HAC1 and IRE1 could promote the nuclear accumulation of Hog1, and increase the cytosolic and bud neck localisation of Slt2, indicating crucial roles of Hog1 and Slt2 in regulating the cellular process in the absence of UPR pathway. Altogether, our findings highlight the significance of these two MAPK pathways of HOG and CWI and their interrelationship with the UPR pathway in responding to cadmium-induced toxicity in budding yeast.

scholarly journals Regulation of Cell-to-Cell Communication and Cell Wall Integrity by a Network of MAP Kinase Pathways and Transcription Factors in Neurospora crassa

Genetics ◽  
2018 ◽  
Vol 209 (2) ◽  
pp. 489-506 ◽  
Author(s):  
Monika S. Fischer ◽  
Vincent W. Wu ◽  
Ji E. Lee ◽  
Ronan C. O’Malley ◽  
N. Louise Glass
Keyword(s):  
Cell Wall ◽  
Transcription Factors ◽  
Map Kinase ◽  
Neurospora Crassa ◽  
Cell Communication ◽  
Cell Wall Integrity ◽  
Map Kinase Pathways
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