Construction of diploid zygotes by interallelic complementation of ade6 in Schizosaccharomyces japonicus

Yeast ◽  
2011 ◽  
Vol 28 (10) ◽  
pp. 747-754 ◽  
Author(s):  
Kanji Furuya ◽  
Hironori Niki
Keyword(s):  
Interallelic Complementation

scholarly journals TEMPERATURE-SENSITIVE MUTATIONS OF THE NOTCH LOCUS IN DROSOPHILA MELANOGASTER

Genetics ◽  
1975 ◽  
Vol 81 (1) ◽  
pp. 143-162 ◽  
Author(s):  
David L Shellenbarger ◽  
J Dawson Mohler
Keyword(s):  
Temperature Shift ◽  
Temperature Sensitive ◽  
Spatial Differences ◽  
Region Temperature ◽  
Complex Locus ◽  
Notch Locus ◽  
Temporal And Spatial ◽  
Chemical Conditions ◽  
Interallelic Complementation ◽  
The Right

ABSTRACT Temperature-conditional mutations of the Notch locus were characterized in an attempt to understand the organization of a "complex locus" and the control of its function in development. Among 21 newly induced Notch alleles, about one-half are temperature-conditional for some effects, and three are temperature-sensitive for viability. One temperature-sensitive lethal, l(1)Nts1, is functionally non-complementing for all known effects of Notch locus mutations and maps at a single site within the locus. Among the existing alleles involved in complex patterns of interallelic complementation, Ax59d5 is found to be temperature-sensitive, while fag, spl, and l(1)N are temperature-independent. Whereas temperature-sensitive alleles map predominantly to the right-most fifth of the locus, fag, spl, and l(1)N are known to map to the left of this region. Temperature-shift experiments demonstrate that fag, spl, and l(1)N cause defects at specific, non-overlapping times in development.—We conclude (1) that the Notch locus is a single cistron (responsible for a single functional molecule, presumably a polypeptide); (2) that the right-most fifth of the locus is, at least in part, the region involved in coding for the Notch product; (3) that the complexity of interallelic complementation is a developmental effect of mutations that cause defects at selected times and spaces, and that complementation occurs because the mutant defects are temporally and spatially non-overlapping; and (4) that mutants express selected defects due to critical temporal and spatial differences in the chemical conditions controlling the synthesis or function of the Notch product. The complexity of the locus appears to reside in controlling the expression (synthesis or function) of the Notch product in development.

scholarly journals Trans-splicing as a Novel Mechanism to Explain Interallelic Complementation in Drosophila

Genetics ◽  
2002 ◽  
Vol 160 (4) ◽  
pp. 1481-1487 ◽  
Author(s):  
Fabien Mongelard ◽  
Mariano Labrador ◽  
Ellen M Baxter ◽  
Tatiana I Gerasimova ◽  
Victor G Corces
Keyword(s):  
Molecular Characterization ◽  
Wild Type ◽  
Functional Protein ◽  
Gypsy Insulator ◽  
Type Function ◽  
Homologous Chromosomes ◽  
Trans Splicing ◽  
Interallelic Complementation

AbstractTwo mutant alleles of the same gene, each located in one of the two homologous chromosomes, may in some instances restore the wild-type function of the gene. This is the case with certain combinations of mutant alleles in the mod(mdg4) gene. This gene encodes several different proteins, including Mod(mdg4)2.2, a component of the gypsy insulator. This protein is encoded by two separate transcription units that can be combined in a trans-splicing reaction to form the mature Mod(mdg4)2.2-encoding RNA. Molecular characterization of complementing alleles shows that they affect the two different transcription units. Flies homozygous for each allele are missing the Mod(mdg4)2.2 protein, whereas wild-type trans-heterozygotes are able to synthesize almost normal levels of the Mod(mdg4)2.2 product. This protein is functional as judged by its ability to form a functional insulator complex. The results suggest that the interallelic complementation in the mod(mdg4) gene is a consequence of trans-splicing between two different mutant transcripts. A conclusion from this observation is that the trans-splicing reaction that takes place between transcripts produced on two different mutant chromosomes ensures wild-type levels of functional protein.

scholarly journals Recombination and complementation between R factors inEscherichia coliK 12

1971 ◽  
Vol 18 (3) ◽  
pp. 287-297 ◽  
Author(s):  
T. J. Foster ◽  
T. G. B. Howe
Keyword(s):  
Escherichia Coli ◽  
Specific Activity ◽  
Chloramphenicol Acetyl Transferase ◽  
Wild Type ◽  
Chloramphenicol Resistance ◽  
Low Level ◽  
R Factor ◽  
Reduced Frequency ◽  
Interallelic Complementation ◽  
R Factors

SUMMARYRecombination between chloramphenicol-sensitive (Cms) mutants of Rl, and R100, has been demonstrated inEscherichia coliK12rec+; it occurs at reduced frequency inrecBandrecC, and is not detectable inreeA, indicating that R factor recombination depends on host functions. Some mutants of R1 also recombine with an R100 mutant in a similar way.recAcells carrying an R1 and an R100 Cmsmutant (hetero-R state) have a low level of chloramphenicol-resistance, and form a chloramphenicol acetyl transferase that has lower specific activity than enzyme from hosts carrying wild-type or recombinant factors. These results suggest the occurrence of interallelic complementation between mutant R factors.

scholarly journals Analysis of the genetic instability induced by nitrous acid in Schizosaccharomyces pombe

1968 ◽  
Vol 12 (1) ◽  
pp. 45-54 ◽  
Author(s):  
N. Loprieno ◽  
A. Abbondandolo ◽  
S. Bonatti ◽  
R. Guglielminetti
Keyword(s):  
Genetic Analysis ◽  
Schizosaccharomyces Pombe ◽  
Genetic Instability ◽  
Nitrous Acid ◽  
Pedigree Analysis ◽  
Genetic Alteration ◽  
Mutagenic Treatment ◽  
Interallelic Complementation ◽  
Cell Colony

A pedigree analysis of several cell-colony generations following a mutagenic treatment with nitrous acid has shown that in S. pombe a genetic instability is produced that replicates several times and produces a mutation in independent lines.It has been shown that the mutants isolated in the progeny of a mosaic colony all contain a genetic alteration that cannot be resolved by genetic analysis and therefore the mutations have occurred at the same genetic site. This finding is confirmed by interallelic complementation and phenotypic analyses.

Super-suppressor induced interallelic complementation

1966 ◽  
Vol 19 (2) ◽  
pp. 583-585 ◽  
Author(s):  
S.G. Inge-Vechtomov ◽  
T.R. Soidla ◽  
S.A. Kožin ◽  
B.V. Simarov
Keyword(s):  
Interallelic Complementation
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