Characterization and expression analysis of a gene cluster for nitrate assimilation from the yeastArxula adeninivorans

Yeast ◽  
2006 ◽  
Vol 26 (2) ◽  
pp. 83-93 ◽  
Author(s):  
Erik Böer ◽  
Anja Schröter ◽  
Rüdiger Bode ◽  
Michael Piontek ◽  
Gotthard Kunze
Keyword(s):  
Gene Cluster ◽  
Expression Analysis ◽  
Nitrate Assimilation

HOXA genes are included in genetic and biologic networks defining human acute T-cell leukemia (T-ALL)

Blood ◽  
2005 ◽  
Vol 106 (1) ◽  
pp. 274-286 ◽  
Author(s):  
Jean Soulier ◽  
Emmanuelle Clappier ◽  
Jean-Michel Cayuela ◽  
Armelle Regnault ◽  
Marina García-Peydró ◽  
...  
Keyword(s):  
T Cell ◽  
Gene Cluster ◽  
Expression Analysis ◽  
Large Scale ◽  
Expression Profiles ◽  
Quantitative Polymerase Chain Reaction ◽  
Ectopic Expression ◽  
Molecular Networks ◽  
Developmental Genes ◽  
Hoxa Genes

Using a combination of molecular cytogenetic and large-scale expression analysis in human T-cell acute lymphoblastic leukemias (T-ALLs), we identified and characterized a new recurrent chromosomal translocation, targeting the major homeobox gene cluster HOXA and the TCRB locus. Real-time quantitative polymerase chain reaction (RQ-PCR) analysis showed that the expression of the whole HOXA gene cluster was dramatically dysregulated in the HOXA-rearranged cases, and also in MLL and CALM-AF10-related T-ALL cases, strongly suggesting that HOXA genes are oncogenic in these leukemias. Inclusion of HOXA-translocated cases in a general molecular portrait of 92 T-ALLs based on large-scale expression analysis shows that this rearrangement defines a new homogeneous subgroup, which shares common biologic networks with the TLX1- and TLX3-related cases. Because T-ALLs derive from T-cell progenitors, expression profiles of the distinct T-ALL subgroups were analyzed with respect to those of normal human thymic subpopulations. Inappropriate use or perturbation of specific molecular networks involved in thymic differentiation was detected. Moreover, we found a significant association between T-ALL oncogenic subgroups and ectopic expression of a limited set of genes, including several developmental genes, namely HOXA, TLX1, TLX3, NKX3-1, SIX6, and TFAP2C. These data strongly support the view that the abnormal expression of developmental genes, including the prototypical homeobox genes HOXA, is critical in T-ALL oncogenesis.

scholarly journals Deletion mapping of theniiAniaD gene region ofAspergillus nidulans

1979 ◽  
Vol 34 (1) ◽  
pp. 19-32 ◽  
Author(s):  
A. Brian Tomsett ◽  
David J. Cove
Keyword(s):  
Fine Structure ◽  
Aspergillus Nidulans ◽  
Gene Cluster ◽  
Nitrate Assimilation ◽  
Gene Region ◽  
Deletion Mapping ◽  
Mutagenic Treatment ◽  
Ofaspergillus Nidulans ◽  
Resistant Mutants ◽  
Genetic Fine Structure

SUMMARYThe genetic fine-structure of theniiAniaD gene region ofAspergillus nidulanshas been studied using deletion mapping. Deletions were identified asniiAniaD double mutants and comprised 1% of spontaneous chlorate-resistant mutants. All such double mutants were shown to involve deletions and their frequency was not increased by mutagenic treatment with eitherN-methyl-N′-nitro-N-nitrosoguanidine or with ultraviolet light. Deletion maps of theniaD andniiA genes have been constructed. A further class of mutation was also mapped using the deletions. Thesecrnmutations, which affect a gene whose function is as yet unknown, map on the centromere proximal side ofniiA. This analysis of a eukaryote gene cluster will provide a framework upon which to base studies of the regulation of the nitrate assimilation pathway.

Inclusion of HOXA Genes in Genetic and Biological Networks Defining Human Acute T-Cell Leukemia.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1110-1110
Author(s):  
Jean Soulier ◽  
Emmanuelle Clappier ◽  
Jean-Michel Cayuela ◽  
Armelle Regnault ◽  
Marina Garcia-Peydro ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cell ◽  
Gene Cluster ◽  
Expression Analysis ◽  
Large Scale ◽  
Lymphoblastic Leukemia ◽  
Ectopic Expression ◽  
Molecular Networks ◽  
Human T Cell ◽  
Hoxa Genes

Abstract We have identified a new recurrent chromosomal translocation, targeting the major homeobox gene cluster HOXA in human T-cell acute lymphoblastic leukemia (T-ALL). Four cases were characterized using a combination of FISH, Southern blot, breakpoint region sequencing, and a large scale expression analysis of a series of T-ALL. Specific RQ-PCR analysis of the HOXA1 to HOXA13 transcripts showed that the whole HOXA gene cluster expression was dramatically deregulated in the HOXA-rearranged cases, and also in the MLL and CALM-AF10-related T-ALL, strongly suggesting that HOXA genes are oncogenic in these types of leukemia. The HOXA-rearranged cases were included in a general portrait of T-ALL based on large scale expression analysis, showing that a new homogeneous T-ALL subgroup is defined by this chromosomal rearrangement. Moreover, patterns of gene expression associated to the distinct T-ALL oncogenic subgroups were compared with gene expression in normal human thymic sub-populations (11 purified sub-populations). Inappropriate use or perturbation of some specific molecular networks involved in thymic differentiation could be detected in the T-ALL cells. Also, we found that abnormal, frequently ectopic, expression of at least one developmental gene, including HOXA, TLX1/HOX11, TLX3/HOX11L2 and a few more, could be identified in most of the T-ALL cases. Our data strongly support the view that the abnormal expression of developmental genes, including the prototypical major homeobox genes HOXA in some cases, is critical in T-ALL oncogenesis.

Cloning of a new antenna gene cluster and expression analysis of the antenna gene family of Rhodopseudomonas palustris

1993 ◽  
Vol 217 (3) ◽  
pp. 867-875 ◽  
Author(s):  
Monier H. TADROS ◽  
Eleni KATSIOU ◽  
Mark A. HOON ◽  
Natalie YURKOVA ◽  
Dipak P. RAMJI
Keyword(s):  
Gene Family ◽  
Gene Cluster ◽  
Expression Analysis ◽  
Rhodopseudomonas Palustris

Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region

Gene ◽  
2000 ◽  
Vol 257 (1) ◽  
pp. 119-130 ◽  
Author(s):  
Lu Gan ◽  
Inyou Lee ◽  
Ryan Smith ◽  
Rhoda Argonza-Barrett ◽  
He Lei ◽  
...  
Keyword(s):  
Serine Protease ◽  
Gene Cluster ◽  
Expression Analysis ◽  
Protease Gene ◽  
Sequencing And Expression ◽  
Chromosome 19Q13 ◽  
Serine Protease Gene
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