Molecular characterization of the 3-phosphoglycerate kinase gene (PGK1) from the methylotrophic yeastPichia pastoris

Yeast ◽  
2005 ◽  
Vol 22 (9) ◽  
pp. 725-737 ◽  
Author(s):  
João Ricardo Moreira de Almeida ◽  
Lídia Maria Pepe de Moraes ◽  
Fernando Araripe Gonçalves Torres
Keyword(s):  
Molecular Characterization ◽  
Phosphoglycerate Kinase ◽  
Kinase Gene ◽  
Phosphoglycerate Kinase Gene

Molecular characterization and regulation of the phosphoglycerate kinase gene from Trichoderma viride

1992 ◽  
Vol 6 (9) ◽  
pp. 1231-1242 ◽  
Author(s):  
G. H. Goldman ◽  
R. A. Geremia ◽  
A. B. Caplan ◽  
S. B. Vila ◽  
R. Villarroel ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Trichoderma Viride ◽  
Phosphoglycerate Kinase ◽  
Kinase Gene ◽  
Phosphoglycerate Kinase Gene

Demethylation of specific sites in the 5' region of the inactive X-linked human phosphoglycerate kinase gene correlates with the appearance of nuclease sensitivity and gene expression

1988 ◽  
Vol 8 (11) ◽  
pp. 4692-4699
Author(s):  
R S Hansen ◽  
N A Ellis ◽  
S M Gartler
Keyword(s):  
Cell Lines ◽  
X Chromosome ◽  
Transcriptional Activation ◽  
Hybrid Cell ◽  
Methylation Status ◽  
Phosphoglycerate Kinase ◽  
Kinase Gene ◽  
Inactive X Chromosome ◽  
Phosphoglycerate Kinase Gene ◽  
Hypoxanthine Guanine Phosphoribosyltransferase

X8/6T2, a hamster-human hybrid cell line which contains an inactive human X chromosome, was treated with 5-azacytidine and selected for derepression of hypoxanthine-guanine phosphoribosyltransferase. Clones were examined for coreactivation of the phosphoglycerate kinase gene (Pgk). Of 68 of these hybrids, approximately 20% expressed measurable human phosphoglycerate kinase (PGK) activity. A 600-base-pair region of the Pgk 5' CpG cluster was examined for the methylation status of eight CCGG sites (site 1 being 5'-most) in a number of PGK-negative and PGK-positive cell lines. The inactive X chromosome is normally methylated at all eight sites, and this was also true for the majority of X8/6T2 cells. However, several PGK-negative hybrids were demethylated in the site 3 to site 6 region. PGK activity correlated with demethylation at both sites 6 and 7. The data for PGK-positive and -negative hybrids indicate that demethylation at or near site 7 was necessary for reactivation of Pgk. Chromatin sensitivity to MspI digestion in the nuclei of male lymphoblastoid cells and several PGK-positive and PGK-negative hybrids was examined. PGK-positive cell lines were hypersensitive to digestion, while PGK-negative hybrids were resistant. Cleavage at sites 6 and 7 was observed in all PGK-positive cell lines at each MspI concentration examined. Sites 7 and 8 were less accessible to digestion than site 6. Cleavage in the site 2 to site 5 region was observable at the lowest MspI concentration. In most PGK-positive hybrids, a nonspecific endogenous nuclease detected the presence of a hypersensitive region spanning at least 450 base pairs, bounded at the 3' end near HpaII site 6. Nuclease hypersensitivity appears to be related to promoter activity, because sites 7 and 8 are in transcribed regions of the gene. These data indicate that specific sites within the CpG cluster have a dominant controlling influence over the Pgk promoter conformation and the transcriptional activation of Pgk.

scholarly journals Nucleotide Sequence of a Wheat Chloroplastic Phosphoglycerate Kinase Gene

1995 ◽  
Vol 107 (4) ◽  
pp. 1483-1484 ◽  
Author(s):  
P. G. Jones ◽  
C. A. Raines ◽  
J. C. Lloyd
Keyword(s):  
Nucleotide Sequence ◽  
Phosphoglycerate Kinase ◽  
Kinase Gene ◽  
Phosphoglycerate Kinase Gene

Molecular characterization of the two-component histidine kinase gene fromMonilinia fructicola

2006 ◽  
Vol 62 (10) ◽  
pp. 991-998 ◽  
Author(s):  
Zhonghua Ma ◽  
Yong Luo ◽  
Themis Michailides
Keyword(s):  
Molecular Characterization ◽  
Histidine Kinase ◽  
Kinase Gene ◽  
Two Component

Isolation and molecular characterization of the swinepox virus thymidine kinase gene

Virology ◽  
1991 ◽  
Vol 183 (2) ◽  
pp. 578-585 ◽  
Author(s):  
J.A. Feller ◽  
R.F. Massung ◽  
P.C. Turner ◽  
E.P.J. Gibbs ◽  
E.O. Bockamp ◽  
...  
Keyword(s):  
Thymidine Kinase ◽  
Molecular Characterization ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Swinepox Virus

Stimulation of Yeast 3-Phosphoglycerate Kinase Gene Promoter by Paraquat

2000 ◽  
Vol 270 (3) ◽  
pp. 1036-1040 ◽  
Author(s):  
Neville Vassallo ◽  
Dolores R. Galea ◽  
William H. Bannister ◽  
Rena Balzan
Keyword(s):  
Phosphoglycerate Kinase ◽  
Gene Promoter ◽  
Kinase Gene ◽  
Phosphoglycerate Kinase Gene ◽  
Stimulation Of
Sign in / Sign up

Export Citation Format

Share Document