Isotope labeling experiments in metabolomics and fluxomics

2012 ◽  
Vol 4 (3) ◽  
pp. 261-272 ◽  
Author(s):  
Sebastian Klein ◽  
Elmar Heinzle
Keyword(s):  
Isotope Labeling ◽  
Isotope Labeling Experiments

Methodology for the Validation of Isotopic Analyses by Mass Spectrometry in Stable-Isotope Labeling Experiments

2018 ◽  
Vol 90 (3) ◽  
pp. 1852-1860 ◽  
Author(s):  
Maud Heuillet ◽  
Floriant Bellvert ◽  
Edern Cahoreau ◽  
Fabien Letisse ◽  
Pierre Millard ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Isotope Labeling ◽  
Stable Isotope Labeling ◽  
Isotopic Analyses ◽  
Isotope Labeling Experiments

KI-Promoted Oxidative Coupling of Styrenes with Indoles under Metal-Free Conditions: Facile Access to C-3 Dicarbonyl Indoles

Synthesis ◽  
2019 ◽  
Vol 51 (18) ◽  
pp. 3511-3519
Author(s):  
Bochao Zhou ◽  
Shiyu Guo ◽  
Zheng Fang ◽  
Zhao Yang ◽  
Chengkou Liu ◽  
...  
Keyword(s):  
Efficient Method ◽  
Oxidative Coupling ◽  
Isotope Labeling ◽  
Cross Coupling ◽  
Metal Free ◽  
Broad Scope ◽  
Plausible Mechanism ◽  
Isotope Labeling Experiments

A new and efficient method for the synthesis of C-3 dicarbonyl indoles via oxidative cross-coupling of styrenes with indoles under metal-free conditions has been developed. Moreover, a broad scope of C-3 dicarbonyl indoles in moderate to good yields were obtained, and a plausible mechanism is proposed based on control and isotope-labeling experiments.

scholarly journals Arcobacter peruensis sp. nov., a Chemolithoheterotroph Isolated from Sulfide- and Organic-Rich Coastal Waters off Peru

2019 ◽  
Vol 85 (24) ◽  
Author(s):  
Cameron M. Callbeck ◽  
Chris Pelzer ◽  
Gaute Lavik ◽  
Timothy G. Ferdelman ◽  
Jon S. Graf ◽  
...  
Keyword(s):  
Coastal Waters ◽  
Carbon Fixation ◽  
Isotope Labeling ◽  
Nitrogen Loss ◽  
Sulfide Oxidation ◽  
Mass Spectrometry Analysis ◽  
Anthropogenic Pressures ◽  
Content Type ◽  
Genes Encoding ◽  
Isotope Labeling Experiments

ABSTRACT Members of the epsilonproteobacterial genus Arcobacter have been identified to be potentially important sulfide oxidizers in marine coastal, seep, and stratified basin environments. In the highly productive upwelling waters off the coast of Peru, Arcobacter cells comprised 3 to 25% of the total microbial community at a near-shore station where sulfide concentrations exceeded 20 μM in bottom waters. From the chemocline where the Arcobacter population exceeded 106 cells ml−1 and where high rates of denitrification (up to 6.5 ± 0.4 μM N day−1) and dark carbon fixation (2.8 ± 0.2 μM C day−1) were measured, we isolated a previously uncultivated Arcobacter species, Arcobacter peruensis sp. nov. (BCCM LMG-31510). Genomic analysis showed that A. peruensis possesses genes encoding sulfide oxidation and denitrification pathways but lacks the ability to fix CO2 via autotrophic carbon fixation pathways. Genes encoding transporters for organic carbon compounds, however, were present in the A. peruensis genome. Physiological experiments demonstrated that A. peruensis grew best on a mix of sulfide, nitrate, and acetate. Isotope labeling experiments further verified that A. peruensis completely reduced nitrate to N2 and assimilated acetate but did not fix CO2, thus coupling heterotrophic growth to sulfide oxidation and denitrification. Single-cell nanoscale secondary ion mass spectrometry analysis of samples taken from shipboard isotope labeling experiments also confirmed that the Arcobacter population in situ did not substantially fix CO2. The efficient growth yield associated with the chemolithoheterotrophic metabolism of A. peruensis may allow this Arcobacter species to rapidly bloom in eutrophic and sulfide-rich waters off the coast of Peru. IMPORTANCE Our multidisciplinary approach provides new insights into the ecophysiology of a newly isolated environmental Arcobacter species, as well as the physiological flexibility within the Arcobacter genus and sulfide-oxidizing, denitrifying microbial communities within oceanic oxygen minimum zones (OMZs). The chemolithoheterotrophic species Arcobacter peruensis may play a substantial role in the diverse consortium of bacteria that is capable of coupling denitrification and fixed nitrogen loss to sulfide oxidation in eutrophic, sulfidic coastal waters. With increasing anthropogenic pressures on coastal regions, e.g., eutrophication and deoxygenation (D. Breitburg, L. A. Levin, A. Oschlies, M. Grégoire, et al., Science 359:eaam7240, 2018, https://doi.org/10.1126/science.aam7240), niches where sulfide-oxidizing, denitrifying heterotrophs such as A. peruensis thrive are likely to expand.

scholarly journals Reconciling Estimates of Cell Proliferation from Stable Isotope Labeling Experiments

2015 ◽  
Vol 11 (10) ◽  
pp. e1004355 ◽  
Author(s):  
Raya Ahmed ◽  
Liset Westera ◽  
Julia Drylewicz ◽  
Marjet Elemans ◽  
Yan Zhang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stable Isotope ◽  
Isotope Labeling ◽  
Stable Isotope Labeling ◽  
Isotope Labeling Experiments

scholarly journals IsoCor: correcting MS data in isotope labeling experiments

2012 ◽  
Vol 28 (9) ◽  
pp. 1294-1296 ◽  
Author(s):  
P. Millard ◽  
F. Letisse ◽  
S. Sokol ◽  
J.-C. Portais
Keyword(s):  
Isotope Labeling ◽  
Isotope Labeling Experiments
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