Viral internal ribosomal entry sites: four classes for one goal

2017 ◽  
Vol 9 (2) ◽  
pp. e1458 ◽  
Author(s):  
Justine Mailliot ◽  
Franck Martin
Keyword(s):  
Internal Ribosomal Entry Sites ◽  
Ribosomal Entry

scholarly journals Genomic determinants of the efficiency of internal ribosomal entry sites of viral and cellular origin

2008 ◽  
Vol 36 (21) ◽  
pp. 6918-6925 ◽  
Author(s):  
Kayole Kazadi ◽  
Corinne Loeuillet ◽  
Samuel Deutsch ◽  
Angela Ciuffi ◽  
Miguel Muñoz ◽  
...  
Keyword(s):  
Internal Ribosomal Entry Sites ◽  
Cellular Origin ◽  
Ribosomal Entry

Translation initiation by factor-independent binding of eukaryotic ribosomes to internal ribosomal entry sites

2005 ◽  
Vol 328 (7) ◽  
pp. 589-605 ◽  
Author(s):  
Andrey V. Pisarev ◽  
Nikolay E. Shirokikh ◽  
Christopher U.T. Hellen
Keyword(s):  
Translation Initiation ◽  
Internal Ribosomal Entry Sites ◽  
Ribosomal Entry

scholarly journals A Role for Drosophila dFoxO and dFoxO 5′UTR Internal Ribosomal Entry Sites during Fasting

PLoS ONE ◽  
2010 ◽  
Vol 5 (7) ◽  
pp. e11521 ◽  
Author(s):  
Eugenia Villa-Cuesta ◽  
Brian T. Sage ◽  
Marc Tatar
Keyword(s):  
Internal Ribosomal Entry Sites ◽  
Ribosomal Entry

scholarly journals Genetically Stable Picornavirus Expression Vectors with Recombinant Internal Ribosomal Entry Sites

2002 ◽  
Vol 76 (17) ◽  
pp. 8966-8972 ◽  
Author(s):  
Andrew T. Dufresne ◽  
Elena Y. Dobrikova ◽  
Stacie Schmidt ◽  
Matthias Gromeier
Keyword(s):  
Genetic Instability ◽  
Design Strategy ◽  
Expression Vectors ◽  
Foreign Gene ◽  
Regulatory Sequences ◽  
Gene Products ◽  
Internal Ribosomal Entry Sites ◽  
Coding Sequences ◽  
Ribosomal Entry

ABSTRACT In many respects, picornaviruses are well suited for their proposed use as immunization vectors. However, their inherent genetic instability hinders application for prophylactic purposes. We demonstrate the improved expression and stability of a heterologous insert through a novel vector design strategy that partially replaces noncoding regulatory sequences with coding sequences for foreign gene products.

scholarly journals The RNA2 5′ leader of Blackcurrant reversion virus mediates efficient in vivo translation through an internal ribosomal entry site mechanism

2007 ◽  
Vol 88 (1) ◽  
pp. 286-297 ◽  
Author(s):  
Alexey Karetnikov ◽  
Kirsi Lehto
Keyword(s):  
Internal Ribosomal Entry Site ◽  
Luciferase Reporter ◽  
Entry Site ◽  
Internal Ribosomal Entry Sites ◽  
Viral Mrnas ◽  
Translational Enhancers ◽  
A Genome ◽  
Reversion Virus ◽  
Ribosomal Entry

The 5′ and 3′ non-translated regions (NTRs) of mRNAs of eukaryotes and their viruses often contain translational enhancers, including internal ribosomal entry sites (IRESs) comprised in the 5′ leaders of many uncapped viral mRNAs. Blackcurrant reversion virus (BRV) has a genome composed of two uncapped, polyadenylated RNAs with relatively short 5′ NTRs, almost devoid of secondary structure. In this work, a role of the RNA2 5′ NTR in translation was studied by using mono- and dicistronic Photinus pyralis and Renilla reniformis luciferase reporter mRNAs in protoplasts of Nicotiana benthamiana. The RNA2 5′ leader was found to confer efficient in vivo translation compared with the control 5′ NTR, and each half of the BRV leader was essential for stimulatory function. Such efficient translational enhancement was mediated, at least in part, through an IRES mechanism. Multiple RNA2 5′ NTR regions, complementary to a fragment of plant 18S rRNA demonstrated previously to be accessible for intermolecular mRNA–rRNA interactions and conserved between eukaryotes, were shown to be important for efficient translation. Similar mRNA–rRNA base-pairing potential was also predicted for the 5′ leaders of other nepoviruses.

scholarly journals The 5′ Leader of the mRNA Encoding the Mouse Neurotrophin Receptor TrkB Contains Two Internal Ribosomal Entry Sites that Are Differentially Regulated

PLoS ONE ◽  
2008 ◽  
Vol 3 (9) ◽  
pp. e3242 ◽  
Author(s):  
Stephanie L. Timmerman ◽  
Jennifer S. Pfingsten ◽  
Jeffrey S. Kieft ◽  
Les A. Krushel
Keyword(s):  
Neurotrophin Receptor ◽  
Internal Ribosomal Entry Sites ◽  
Ribosomal Entry
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