Control of germline stem cell self-renewal and differentiation in the Drosophila ovary: concerted actions of niche signals and intrinsic factors

2012 ◽  
Vol 2 (2) ◽  
pp. 261-273 ◽  
Author(s):  
Xie Ting
Keyword(s):  
Stem Cell ◽  
Germline Stem Cell ◽  
Self Renewal ◽  
Intrinsic Factors ◽  
Drosophila Ovary

scholarly journals The Osa-Containing SWI/SNF Chromatin-Remodeling Complex Is Required in the Germline Differentiation Niche for Germline Stem Cell Progeny Differentiation

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 363
Author(s):  
Xiaolong Hu ◽  
Mengjie Li ◽  
Xue Hao ◽  
Yi Lu ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bmp Signaling ◽  
Germline Stem Cell ◽  
Dependent Manner ◽  
Self Renewal ◽  
Cellular Processes ◽  
Lineage Differentiation ◽  
Chromatin Remodeling Complex ◽  
Germline Differentiation ◽  
Drosophila Ovary

The Drosophila ovary is recognized as a powerful model to study stem cell self-renewal and differentiation. Decapentaplegic (Dpp) is secreted from the germline stem cell (GSC) niche to activate Bone Morphogenic Protein (BMP) signaling in GSCs for their self-renewal and is restricted in the differentiation niche for daughter cell differentiation. Here, we report that Switch/sucrose non-fermentable (SWI/SNF) component Osa depletion in escort cells (ECs) results in a blockage of GSC progeny differentiation. Further molecular and genetic analyses suggest that the defective germline differentiation is partially attributed to the elevated dpp transcription in ECs. Moreover, ectopic Engrailed (En) expression in osa-depleted ECs partially contributes to upregulated dpp transcription. Furthermore, we show that Osa regulates germline differentiation in a Brahma (Brm)-associated protein (BAP)-complex-dependent manner. Additionally, the loss of EC long cellular processes upon osa depletion may also partly contribute to the germline differentiation defect. Taken together, these data suggest that the epigenetic factor Osa plays an important role in controlling EC characteristics and germline lineage differentiation.

scholarly journals Histone H1-mediated epigenetic regulation controls germline stem cell self-renewal by modulating H4K16 acetylation

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Jin Sun ◽  
Hui-Min Wei ◽  
Jiang Xu ◽  
Jian-Feng Chang ◽  
Zhihao Yang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epigenetic Regulation ◽  
Histone H1 ◽  
Germline Stem Cell ◽  
Self Renewal ◽  
H4k16 Acetylation

scholarly journals Loss-of-Function Screen Reveals Novel Regulators Required for Drosophila Germline Stem Cell Self-Renewal

2012 ◽  
Vol 2 (3) ◽  
pp. 343-351 ◽  
Author(s):  
Yalan Xing ◽  
Irina Kurtz ◽  
Manisha Thuparani ◽  
Jillian Legard ◽  
Hannele Ruohola-Baker
Keyword(s):  
Stem Cell ◽  
Germline Stem Cell ◽  
Loss Of Function ◽  
Self Renewal

scholarly journals A Drosophila Chromatin Factor Interacts With the Piwi-Interacting RNA Mechanism in Niche Cells to Regulate Germline Stem Cell Self-Renewal

Genetics ◽  
2010 ◽  
Vol 186 (2) ◽  
pp. 573-583 ◽  
Author(s):  
Tora K. Smulders-Srinivasan ◽  
Akos Szakmary ◽  
Haifan Lin
Keyword(s):  
Stem Cell ◽  
Germline Stem Cell ◽  
Self Renewal

scholarly journals Aubergine Controls Germline Stem Cell Self-Renewal and Progeny Differentiation via Distinct Mechanisms

2017 ◽  
Vol 41 (2) ◽  
pp. 157-169.e5 ◽  
Author(s):  
Xing Ma ◽  
Xiujuan Zhu ◽  
Yingying Han ◽  
Benjamin Story ◽  
Trieu Do ◽  
...  
Keyword(s):  
Stem Cell ◽  
Germline Stem Cell ◽  
Self Renewal

scholarly journals Integrins control the positioning and proliferation of follicle stem cells in the Drosophila ovary

2008 ◽  
Vol 182 (4) ◽  
pp. 801-815 ◽  
Author(s):  
Alana M. O'Reilly ◽  
Hsiu-Hsiang Lee ◽  
Michael A. Simon
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Adult Stem Cells ◽  
Cell Communication ◽  
Self Renewal ◽  
Ovarian Stem Cells ◽  
Stem Cell Populations ◽  
Integrin Ligand ◽  
Follicle Stem Cells ◽  
Drosophila Ovary

Adult stem cells are maintained in specialized microenvironments called niches, which promote self-renewal and prevent differentiation. In this study, we show that follicle stem cells (FSCs) in the Drosophila melanogaster ovary rely on cues that are distinct from those of other ovarian stem cells to establish and maintain their unique niche. We demonstrate that integrins anchor FSCs to the basal lamina, enabling FSCs to maintain their characteristic morphology and position. Integrin-mediated FSC anchoring is also essential for proper development of differentiating prefollicle cells that arise from asymmetrical FSC divisions. Our results support a model in which FSCs contribute to the formation and maintenance of their own niche by producing the integrin ligand, laminin A (LanA). Together, LanA and integrins control FSC proliferation rates, a role that is separable from their function in FSC anchoring. Importantly, LanA-integrin function is not required to maintain other ovarian stem cell populations, demonstrating that distinct pathways regulate niche–stem cell communication within the same organ.

scholarly journals eIF4A controls germline stem cell self-renewal by directly inhibiting BAM function in theDrosophilaovary

2009 ◽  
Vol 106 (28) ◽  
pp. 11623-11628 ◽  
Author(s):  
Run Shen ◽  
Changjiang Weng ◽  
Junjing Yu ◽  
Ting Xie
Keyword(s):  
Stem Cell ◽  
Germline Stem Cell ◽  
Self Renewal

Analysis of GPI-anchored proteins involved in germline stem cell proliferation in the Caenorhabditis elegans germline stem cell niche

2020 ◽  
Vol 168 (6) ◽  
pp. 589-602
Author(s):  
Marika Rikitake ◽  
Ayako Matsuda ◽  
Daisuke Murata ◽  
Katsufumi Dejima ◽  
Kazuko H Nomura ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Caenorhabditis Elegans ◽  
Lipid Rafts ◽  
Stem Cell Niche ◽  
Germline Stem Cell ◽  
Self Renewal ◽  
Stem Cell Proliferation ◽  
Cell Niche ◽  
Germline Stem Cell Niche

Abstract Stem cells divide and undergo self-renewal depending on the signals received from the stem cell niche. This phenomenon is indispensable to maintain tissues and organs in individuals. However, not all the molecular factors and mechanisms of self-renewal are known. In our previous study, we reported that glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) synthesized in the distal tip cells (DTCs; the stem cell niche) are essential for germline stem cell proliferation in Caenorhabditis elegans. Here, we characterized the GPI-APs required for proliferation. We selected and verified the candidate GPI-APs synthesized in DTCs by RNA interference screening and found that F57F4.3 (GFI-1), F57F4.4 and F54E2.1 are necessary for germline proliferation. These proteins are likely involved in the same pathway for proliferation and activated by the transcription factor PQM-1. We further provided evidence suggesting that these GPI-APs act through fatty acid remodelling of the GPI anchor, which is essential for association with lipid rafts. These findings demonstrated that GPI-APs, particularly F57F4.3/4 and F54E2.1, synthesized in the germline stem cell niche are located in lipid rafts and involved in promoting germline stem cell proliferation in C. elegans. The findings may thus shed light on the mechanisms by which GPI-APs regulate stem cell self-renewal.

Sign in / Sign up

Export Citation Format

Share Document