Self-incompatibility in Petunia : a self/nonself-recognition mechanism employing S -locus F-box proteins and S-RNase to prevent inbreeding

2011 ◽  
Vol 1 (2) ◽  
pp. 267-275 ◽  
Author(s):  
Ning Wang ◽  
Teh-Hui Kao
Keyword(s):  
Self Incompatibility ◽  
S Locus ◽  
Recognition Mechanism ◽  
Nonself Recognition

Sequence and Structural Diversity of the S Locus Genes From Different Lines With the Same Self-Recognition Specificities in Brassica oleracea

Genetics ◽  
2000 ◽  
Vol 154 (1) ◽  
pp. 413-420 ◽  
Author(s):  
Makoto Kusaba ◽  
Masanori Matsushita ◽  
Keiichi Okazaki ◽  
Yoko Satta ◽  
Takeshi Nishio
Keyword(s):  
Structural Diversity ◽  
Receptor Protein ◽  
Self Incompatibility ◽  
S Locus ◽  
High Similarity ◽  
Putative Receptor ◽  
Recognition Specificity ◽  
Polymorphic Genes ◽  
Self Recognition ◽  
Self Fertilization

Abstract Self-incompatibility (SI) is a mechanism for preventing self-fertilization in flowering plants. In Brassica, it is controlled by a single multi-allelic locus, S, and it is believed that two highly polymorphic genes in the S locus, SLG and SRK, play central roles in self-recognition in stigmas. SRK is a putative receptor protein kinase, whose extracellular domain exhibits high similarity to SLG. We analyzed two pairs of lines showing cross-incompatibility (S2 and S2-b; S13 and S13-b). In S2 and S2-b, SRKs were more highly conserved than SLGs. This was also the case with S13 and S13-b. This suggests that the SRKs of different lines must be conserved for the lines to have the same self-recognition specificity. In particular, SLG2-b showed only 88.5% identity to SLG2, which is comparable to that between the SLGs of different S haplotypes, while SRK2-b showed 97.3% identity to SRK2 in the S domain. These findings suggest that the SLGs in these S haplotypes are not important for self-recognition in SI.

Visualization of the S-locus region in Ipomoea trifida: toward positional cloning of self-incompatibility genes

2004 ◽  
Vol 12 (5) ◽  
pp. 475-481 ◽  
Author(s):  
Go Suzuki ◽  
Saiko Tanaka ◽  
Maki Yamamoto ◽  
Rubens Norio Tomita ◽  
Yasuo Kowyama ◽  
...  
Keyword(s):  
Positional Cloning ◽  
Self Incompatibility ◽  
S Locus ◽  
Ipomoea Trifida

scholarly journals Self-Incompatibility in the Genus Arabidopsis: Characterization of the S Locus in the Outcrossing A. lyrata and Its Autogamous Relative A. thaliana

2001 ◽  
Vol 13 (3) ◽  
pp. 627-643 ◽  
Author(s):  
Makoto Kusaba ◽  
Kathleen Dwyer ◽  
Jennifer Hendershot ◽  
Julia Vrebalov ◽  
June B. Nasrallah ◽  
...  
Keyword(s):  
Self Incompatibility ◽  
S Locus

scholarly journals S-Locus F-Box Proteins Are Solely Responsible for S-RNase-Based Self-Incompatibility of Petunia Pollen

2018 ◽  
Vol 30 (12) ◽  
pp. 2959-2972 ◽  
Author(s):  
Linhan Sun ◽  
Justin S. Williams ◽  
Shu Li ◽  
Lihua Wu ◽  
Wasi A. Khatri ◽  
...  
Keyword(s):  
Self Incompatibility ◽  
S Locus

Identification and characterization of BoPUB3: a novel interaction protein with S-locus receptor kinase in Brassica oleracea L.

2019 ◽  
Vol 51 (7) ◽  
pp. 723-733 ◽  
Author(s):  
Songmei Shi ◽  
Qiguo Gao ◽  
Tonghong Zuo ◽  
Zhenze Lei ◽  
Quanming Pu ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Signaling Pathway ◽  
Chromosomal Region ◽  
The Self ◽  
Mature Stage ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
Phylogenic Tree ◽  
Brassica Oleracea L

Abstract Armadillo repeat containing 1 (ARC1) is phosphorylated by S-locus receptor kinase (SRK) and functions as a positive regulator in self-incompatibility response of Brassica. However, ARC1 only causes partial breakdown of the self-incompatibility response, and other SRK downstream factors may also participate in the self-incompatibility signaling pathway. In the present study, to search for SRK downstream targets, a plant U-box protein 3 (BoPUB3) was identified from the stigma of Brassica oleracea L. BoPUB3 was highly expressed in the stigma, and its expression was increased with the stigma development and reached to the highest level in the mature-stage stigma. BoPUB3, a 76.8-kDa protein with 697 amino acids, is a member of the PUB-ARM family and contains three domain characteristics of BoARC1, including a U-box N-terminal domain, a U-box motif, and a C-terminal arm repeat domain. The phylogenic tree showed that BoPUB3 was close to BoARC1. The synteny analysis revealed that B. oleracea chromosomal region containing BoPUB3 had high synteny with the Arabidopsis thaliana chromosomal region containing AtPUB3 (At3G54790). In addition, the subcellular localization analysis showed that BoPUB3 primarily localized in the plasma membrane and also in the cytoplasm. The combination of the yeast two-hybrid and in vitro binding assay showed that both BoPUB3 and BoARC1 could interact with SRK kinase domain, and SRK showed much higher level of β-galactosidase activity in its interaction with BoPUB3 than with BoARC1. These results implied that BoPUB3 is a novel interactor with SRK, which lays a basis for further research on whether PUB3 participates in the self-incompatibility signaling pathway.

scholarly journals A Dual Role for the S-Locus Receptor Kinase in Self-Incompatibility and Pistil Development Revealed by an Arabidopsis rdr6 Mutation

2009 ◽  
Vol 21 (9) ◽  
pp. 2642-2654 ◽  
Author(s):  
Titima Tantikanjana ◽  
Noreen Rizvi ◽  
Mikhail E. Nasrallah ◽  
June B. Nasrallah
Keyword(s):  
Dual Role ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
Pistil Development

scholarly journals Molecular Characterization of S Locus Genes, SLG and SRK, in a Pollen-Recessive Self-Incompatibility Haplotype of Brassica rapa L

Genetics ◽  
1998 ◽  
Vol 149 (3) ◽  
pp. 1587-1597 ◽  
Author(s):  
Katsunori Hatakeyama ◽  
Takeshi Takasaki ◽  
Masao Watanabe ◽  
Kokichi Hinata
Keyword(s):  
Brassica Rapa ◽  
Genomic Dna ◽  
Transmembrane Domain ◽  
Brassica Species ◽  
Self Incompatibility ◽  
S Locus ◽  
Intron Sequence ◽  
High Degree ◽  
Degree Of Similarity

Abstract In Brassica species that exhibit self-incompatibility, two genes, SLG and SRK, at the S locus are involved in the recognition reaction with self and non-self pollen. From a pollen-recessive S29 haplotype of Brassica rapa, both cDNA and genomic DNA clones for these two genes were isolated and characterized. The nucleotide sequence for the S domain of SRK29 showed a high degree of similarity with that of SLG29, and they belong to Class II type. RNA gel blot analysis showed that the transcript of SLG29 consisted of the first and second exons, and no other transcript containing any part of the intron sequence was detected. Because no transmembrane domain was encoded by the second exon of SLG29, SLG29 was designated a secreted type glycoprotein. SLGs of two other pollen-recessive haplotypes, S40 and S44, of B. rapa also had a similar structure to that of SLG29. Previously, SLG2 from a pollen-recessive haplotype, S2, of Brassica oleracea was found to produce two different transcripts, one for the secreted type glycoprotein and the other for a putative membrane-anchored form of SLG. Therefore, the nature of these SLGs from pollen-recessive haplotypes of B. rapa is different from that of SLG2 of B. oleracea.

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