scholarly journals Moving in the mesoscale: Understanding the mechanics of cytoskeletal molecular motors by combining mesoscale simulations with imaging

2021 ◽  
Author(s):  
Molly S. C. Gravett ◽  
Ryan C. Cocking ◽  
Alistair P. Curd ◽  
Oliver Harlen ◽  
Joanna Leng ◽  
...  
Keyword(s):  
Molecular Motors ◽  
Mesoscale Simulations

Video-microscopic measurement of cell-substratum traction forces generated by locomoting keratocytes

1993 ◽  
Vol 51 ◽  
pp. 188-189
Author(s):  
Tim Oliver ◽  
Michelle Leonard ◽  
Juliet Lee ◽  
Akira Ishihara ◽  
Ken Jacobson
Keyword(s):  
Silicone Rubber ◽  
Molecular Motors ◽  
Video Frame ◽  
Traction Forces ◽  
Cell Traction Forces ◽  
Latex Beads ◽  
Cell Traction ◽  
Local Cell ◽  
Microscopic Measurement ◽  
Kinetics Of

We are using video-enhanced light microscopy to investigate the pattern and magnitude of forces that fish keratocytes exert on flexible silicone rubber substrata. Our goal is a clearer understanding of the way molecular motors acting through the cytoskeleton co-ordinate their efforts into locomotion at cell velocities up to 1 μm/sec. Cell traction forces were previously observed as wrinkles(Fig.l) in strong silicone rubber films by Harris.(l) These forces are now measureable by two independant means.In the first of these assays, weakly crosslinked films are made, into which latex beads have been embedded.(Fig.2) These films report local cell-mediated traction forces as bead displacements in the plane of the film(Fig.3), which recover when the applied force is released. Calibrated flexible glass microneedles are then used to reproduce the translation of individual beads. We estimate the force required to distort these films to be 0.5 mdyne/μm of bead movement. Video-frame analysis of bead trajectories is providing data on the relative localisation, dissipation and kinetics of traction forces.

scholarly journals 3P182 AFM observations of flagella of the tethered cell on surfaces(Molecular motors,Poster Presentations)

2007 ◽  
Vol 47 (supplement) ◽  
pp. S248
Author(s):  
Takuji Mashimo ◽  
Tomoyuki Yoshino ◽  
Shin-Ichi Aizawa
Keyword(s):  
Molecular Motors ◽  
Poster Presentations

scholarly journals A model of processive walking and slipping of kinesin-8 molecular motors

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ping Xie
Keyword(s):  
Single Molecule ◽  
Molecular Motors ◽  
External Load ◽  
Molecular Motor ◽  
Velocity Versus ◽  
Stick Slip ◽  
Load Dependence ◽  
Chemomechanical Coupling ◽  
Similarities And Differences ◽  
Stick Slip Motion

AbstractKinesin-8 molecular motor can move with superprocessivity on microtubules towards the plus end by hydrolyzing ATP molecules, depolymerizing microtubules. The available single molecule data for yeast kinesin-8 (Kip3) motor showed that its superprocessive movement is frequently interrupted by brief stick–slip motion. Here, a model is presented for the chemomechanical coupling of the kinesin-8 motor. On the basis of the model, the dynamics of Kip3 motor is studied analytically. The analytical results reproduce quantitatively the available single molecule data on velocity without including the slip and that with including the slip versus external load at saturating ATP as well as slipping velocity versus external load at saturating ADP and no ATP. Predicted results on load dependence of stepping ratio at saturating ATP and load dependence of velocity at non-saturating ATP are provided. Similarities and differences between dynamics of kinesin-8 and that of kinesin-1 are discussed.

scholarly journals Correction to: Effective behavior of cooperative and nonidentical molecular motors

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Joseph J. Klobusicky ◽  
John Fricks ◽  
Peter R. Kramer
Keyword(s):  
Molecular Motors ◽  
Effective Behavior

Mechanistic analysis of light-driven overcrowded alkene-based molecular motors by multiscale molecular simulations

2021 ◽  
Vol 23 (14) ◽  
pp. 8525-8540
Author(s):  
Mudong Feng ◽  
Michael K. Gilson
Keyword(s):  
Molecular Dynamics ◽  
Excited State ◽  
Molecular Dynamics Simulations ◽  
Ground State ◽  
Molecular Motors ◽  
Motor Performance ◽  
Molecular Simulations ◽  
Mechanistic Analysis ◽  
Dynamics Simulations ◽  
Shed Light

Ground-state and excited-state molecular dynamics simulations shed light on the rotation mechanism of small, light-driven molecular motors and predict motor performance. How fast can they rotate; how much torque and power can they generate?

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