scholarly journals Epididymis incision as a method to collect epididymal sperm cells in alpacas

2021 ◽  
Author(s):  
Marlen Auer ◽  
Henrik Wagner ◽  
Klaus Failing ◽  
Axel Wehrend
Keyword(s):  
Sperm Cells ◽  
Epididymal Sperm

32 An approach to invitro oocyte maturation and fertilization in threatened Saharan Dorcas gazelle (Gazella dorcas osiris) using frozen-thawed epididymal sperm cells

2021 ◽  
Vol 33 (2) ◽  
pp. 123
Author(s):  
M. Ruiz-Conca ◽  
J. Gardela ◽  
M. Álvarez-Rodríguez ◽  
H. Fernández-Bellon ◽  
M. López-Béjar
Keyword(s):  
Academic Staff ◽  
Polar Body ◽  
Computer Assisted ◽  
Sperm Cells ◽  
Epididymal Sperm ◽  
Sperm Analysis ◽  
First Polar Body ◽  
Semen Extender ◽  
Polar Body Extrusion ◽  
Humidified Air

The Dorcas gazelle (Gazella dorcas), in the family Bovidae, is one of the smallest species of the genus Gazella. Its population is decreasing and it is classified as vulnerable by the International Union of Conservation of Nature Red List of Threatened Species. The subspecies Saharan Dorcas gazelle (G. dorcas osiris), originating in the central-western Saharan and Sahel regions, is almost extinct in the wild because of over-hunting. Thereon, assisted reproductive techniques (ARTs) could help in its population management. Here, we performed invitro maturation (IVM) of oocytes recovered from an adult female of Saharan Dorcas gazelle (10 years old) to attempt IVF with frozen-thawed epididymal sperm cells from an adult male (9 years old) of the same subspecies, following protocols for ARTs in cattle. Epididymal sperm was obtained after postmortem examination by cannulation of vas deferens with commercial semen extender Gent A (Minitub). Motility features were assessed using a computer-assisted sperm analysis system (Proiser SL), and viability was evaluated using eosin/nigrosin staining. After collection, viability (88.3%), total (17.8%) and progressive (8.7%) motility were assessed. A total of 820.5×106 sperm cells was obtained and diluted (1:1) in a semen extender with permeable cryoprotectants (Gent B, Minitub). Then, the sample was refrigerated in straws (200×106 mL−1; 0.5mL) at 4°C for 1h and exposed to liquid nitrogen vapors (LN2) for 10min before being directly plunged into and kept on LN2. A total of 35 oocytes were recovered by slicing, 27 of which were selected for IVM (24h, 38.5°C, 5% CO2 in humidified air). Motile sperm were obtained by centrifugation of thawed semen (10min, 1000×g) by performing a density gradient (BoviPure, Nidacon). Post-thawing viability (72.9%) and total (14%) and progressive (4%) motility were evaluated just before IVF. After IVM, 17 oocytes were incubated for IVF for 20h with 1×106 thawed sperm cells mL−1 (although the first polar body extrusion was observed only in 5 of the 17 oocytes before IVF; 29.4%). Also, 7 oocytes selected for IVM were vitrified and kept for the genetic bank, and 3 were fixed. Thereafter, all oocytes that underwent IVF were incubated for invitro embryo culture (38.5°C, 5% CO2, 5% O2 in humidified air) to avoid discarding any potential zygote. Cleavage was assessed at 48h post-IVF. From those, only 2 oocytes successfully achieved IVF out of the 5 that showed first polar body extrusion (40%), although cleavage was asymmetric and embryos did not progress. The rest of oocytes (12) did not achieved IVM, IVF, or cleavage. Results suggest that optimal protocols and appropriate individualized conditions for vulnerable nondomestic ungulates are required for successful ARTs. Future efforts are needed to improve preservation strategies for the gametes/embryo conservation of endangered species. JG and MRC contributed equally to this work. JG is supported by AGAUR (2018 FI_B 00236) and MRC by FPU (Training for Academic Staff) (FPU15/06029).

scholarly journals Isolation and characterization of a small putative zinc finger protein from cuttlefish epididymal sperm cells

1994 ◽  
Vol 220 (2) ◽  
pp. 463-468 ◽  
Author(s):  
Annie MARTIN-PONTHIEU ◽  
Daniele WOUTERS-TYROU ◽  
Barbara PUDLO ◽  
Eric BUISINE ◽  
Pierre SAUTIERE
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Sperm Cells ◽  
Epididymal Sperm ◽  
Isolation And Characterization ◽  
Finger Protein ◽  
Putative Zinc Finger

Daily exposure to summer temperatures affects the motile subpopulation structure of epididymal sperm cells but not male fertility in an in vivo rabbit model

2015 ◽  
Vol 84 (3) ◽  
pp. 384-389 ◽  
Author(s):  
M.J. Maya-Soriano ◽  
E. Taberner ◽  
M. Sabés-Alsina ◽  
J. Ramon ◽  
O. Rafel ◽  
...  
Keyword(s):  
Male Fertility ◽  
Rabbit Model ◽  
Sperm Cells ◽  
Epididymal Sperm ◽  
Daily Exposure ◽  
Summer Temperatures

Succesful postmortem refrigeration of epididymal sperm cells from a komodo dragon

Cryobiology ◽  
2018 ◽  
Vol 85 ◽  
pp. 177-178
Author(s):  
Mateo Ruiz-Conca ◽  
Jaume Gardela ◽  
Josep Estruch ◽  
Sergi Olvera ◽  
Yedra Feltrer ◽  
...  
Keyword(s):  
Sperm Cells ◽  
Epididymal Sperm

Ultrastructure of Testicular Spermatozoon of the Fish Oreochromis Niloticus

1988 ◽  
Vol 46 ◽  
pp. 278-279
Author(s):  
T. Guha ◽  
A. Q. Siddiqui ◽  
P. F. Prentis
Keyword(s):  
Electron Microscope ◽  
Oreochromis Niloticus ◽  
Osmium Tetroxide ◽  
Sperm Cells ◽  
Adult Fish ◽  
Testicular Sperm ◽  
Thin Sections ◽  
Important Fish ◽  
Testicular Spermatozoon ◽  
Diamond Knife

Tilapia, Oreochromis niloticus, is an economically important fish in Saudi Arabia. Elucidation of reproductive biology of this species is necessary for successful breeding program. In this paper we describe fine structure of testicular sperm cells in O, niloticus.Testes from young adult fish were fixed in gluteraldehyde (2%) and osmium tetroxide (1%), both in cacodyl ate buffer. Specimens were processed in the conventional way for electron microscopy and thin sections of tissues (obtained by cutting the blocks with a diamond knife) were stained by ura- nyl acetate and lead citrate. These were examined in a Carl Zeiss electron microscope operated at 40 kV to 60 kV. Sperm cells were obtained from testes by squeezing them in cacodyl ate buffer. They were fixed in gluteraldehyde (2%) in the same buffer, air dried, gold coated and then examined in a Philips scanning electron microscope (SEM) operated at 25kV.The spermatozoon of O. niloticus is consisting of head, midpiece and tail (Fig. 1).

PATTERN OF 17-KETOSTEROID URINARY EXCRETION IN INFERTILE MEN

1963 ◽  
Vol 42 (1) ◽  
pp. 97-100
Author(s):  
Mirjam Furuhjelm ◽  
Birgit Johnson ◽  
C.-G. Lagergren
Keyword(s):  
Urinary Excretion ◽  
Sperm Cells ◽  
Infertile Men ◽  
Abnormal Cells

ABSTRACT The pattern of the 17-ketosteroid excretion determined according to Johnsen (1956) in a group of 33 sterile men was compared with that in another group of 14 men with proved fertility, and no difference was found. No correlation between 17-ketosteroid excretion and the concentration of sperm cells or the percentage of abnormal cells could be detected. The results are discussed.

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