scholarly journals Molecular identification of Babesia canis canis genotype A in a dog from Iran

2021 ◽  
Author(s):  
Milad Ghasemzade ◽  
Bijan Esmaeilnejad ◽  
Siamak Asri‐Rezaei ◽  
Mojtaba Hadian
Keyword(s):  
Molecular Identification ◽  
Babesia Canis ◽  
Babesia Canis Canis ◽  
Genotype A

Molecular characterisation of Babesia canis canis and Babesia canis vogeli from naturally infected European dogs

2002 ◽  
Vol 106 (4) ◽  
pp. 285-292 ◽  
Author(s):  
Simone M. Cacciò ◽  
Boris Antunovic ◽  
Annabella Moretti ◽  
Vittorio Mangili ◽  
Albert Marinculic ◽  
...  
Keyword(s):  
Babesia Canis ◽  
Molecular Characterisation ◽  
Babesia Canis Canis

The prevention of transmission of Babesia canis canis by Dermacentor reticulatus ticks to dogs using a novel combination of fipronil, amitraz and (S)-methoprene

2011 ◽  
Vol 179 (4) ◽  
pp. 343-350 ◽  
Author(s):  
Frans Jongejan ◽  
Josephus J. Fourie ◽  
S. Theodore Chester ◽  
Coralie Manavella ◽  
Yasmina Mallouk ◽  
...  
Keyword(s):  
Dermacentor Reticulatus ◽  
Babesia Canis ◽  
Babesia Canis Canis

The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs

2013 ◽  
Vol 16 (1) ◽  
pp. 131-133 ◽  
Author(s):  
Ł. Adaszek ◽  
M. Jankowska ◽  
M. Kalinowski ◽  
T. Banach ◽  
D. Wułupek ◽  
...  
Keyword(s):  
High Specificity ◽  
Isothermal Amplification ◽  
Lamp Method ◽  
Babesia Canis ◽  
Canine Babesiosis ◽  
Loop Mediated Isothermal Amplification ◽  
Specificity And Sensitivity ◽  
Amplification Assay ◽  
Analytical Laboratories ◽  
Babesia Canis Canis

Abstract The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that this method has high specificity and sensitivity and can be applied in analytical laboratories in diagnosis of canine babesiosis.

Molecular evidence of natural infection with Babesia canis canis in Croatia

2010 ◽  
Vol 58 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Mirna Brkljačić ◽  
Vesna Matijatko ◽  
Ivana Kiš ◽  
Nada Kučer ◽  
Jadranka Foršek ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Genetic Variant ◽  
Natural Infection ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Babesia Canis ◽  
Nucleotide Difference ◽  
Dark Urine ◽  
Blood Smears ◽  
Babesia Canis Canis

The aim of the present study was to detect and characterise the species and subspecies of Babesia spp. that cause canine babesiosis in Croatia. Twenty-eight dogs with typical signs of babesiosis (lethargy, anorexia, fever, dark urine and thrombocytopenia) were included in this study. Their blood smears showed the presence of Babesia canis . The results showed the detection of one subspecies, namely Babesia canis canis using PCR, and subsequent sequence analysis demonstrated portions of the nss rRNA gene in 27 out of 28 samples. Sequence analysis of the isolates showed 100% identity in 11 samples, 99.7% identity (one nucleotide difference) in 11 samples and 99.4% identity (two nucleotides difference) in 5 samples with B. canis canis . The results of this study confirm the presence of B. canis canis in infected dogs in Croatia and demonstrate a slightly new genetic variant of Babesia subspecies.

Postmortem small babesia-like morphology of Babesia canis — Short communication

2011 ◽  
Vol 59 (4) ◽  
pp. 427-432 ◽  
Author(s):  
Zoltán Demeter ◽  
Elena Palade ◽  
Éva Balogh ◽  
Csaba Jakab ◽  
Róbert Farkas ◽  
...  
Keyword(s):  
Causative Agent ◽  
18S Rrna Gene ◽  
Severe Anaemia ◽  
Rrna Gene ◽  
Babesia Canis ◽  
Post Mortem ◽  
Tissue Samples ◽  
Tissue Sections ◽  
Sequence Identity ◽  
Babesia Canis Canis

Here we report a case of canine babesiosis with unusual morphology of the causative agent. A male, seven-week-old Labrador retriever puppy, exhibiting severe anaemia and haemoglobinuria, was presented at the Clinic of Internal Medicine in February 2011. The puppy was euthanised. The most relevant pathological changes were icterus, severe splenomegaly, generalised lymphadenopathy and haemoglobin nephrosis. Samples were collected from various organs for histology within one hour post mortem. Impression smears were also prepared from the spleen after overnight storage at 4 °C. Tissue sections and smears showed the presence of multiple, coccoid intraerythrocytic bodies that measured 1–2 μm and resembled small babesiae. No large piroplasms were seen. DNA was extracted from the spleen, and a conventional PCR was performed for the amplification of a 450-bp region of the 18S rRNA gene of piroplasms. The causative agent was identified as Babesia canis canis, with 99% sequence identity to other European isolates. Sequence identity to B. gibsoni was only 91%. This is the first account to verify that the morphology of the large canine piroplasm, B. canis, can be uniformly small babesia-like post mortem or following the storage of tissue samples.

First case of babesiosis caused by Babesia canis canis in a dog from Norway

2010 ◽  
Vol 171 (3-4) ◽  
pp. 350-353 ◽  
Author(s):  
Øivind Øines ◽  
Katarina Storli ◽  
Hege Brun-Hansen
Keyword(s):  
Babesia Canis ◽  
First Case ◽  
Babesia Canis Canis

Assessment of primers designed for the subspecies-specific discrimination among Babesia canis canis, Babesia canis vogeli and Babesia canis rossi by PCR assay

2008 ◽  
Vol 152 (1-2) ◽  
pp. 16-20 ◽  
Author(s):  
Sabrina Castilho Duarte ◽  
Guido Fontgalland Coelho Linhares ◽  
Tatiana Nunes Romanowsky ◽  
Osvaldo José da Silveira Neto ◽  
Ligia Miranda Ferreira Borges
Keyword(s):  
Babesia Canis ◽  
Pcr Assay ◽  
Babesia Canis Canis

In-clinic laboratory diagnosis of canine babesiosis (Babesia canis canis) for veterinary practitioners in Central Europe

2012 ◽  
Vol 40 (02) ◽  
pp. 87-94 ◽  
Author(s):  
M. Leschnik ◽  
E. Hooijberg ◽  
A. Tichy ◽  
E. Leidinger ◽  
G. Kirtz
Keyword(s):  
Reticulocyte Count ◽  
Climatic Factors ◽  
Laboratory Diagnosis ◽  
Climatic Conditions ◽  
Babesia Canis ◽  
Canine Babesiosis ◽  
Vector Activity ◽  
Red Blood Cell Count ◽  
Haematological Changes ◽  
Babesia Canis Canis

Summary Objective: Haematological changes in dogs and climatic conditions favourable for the vector may assist in the quick in-house diagnosis of canine babesiosis. Material and methods: Blood samples from 358 dogs suspected to have canine babesiosis were evaluated. The diagnosis was confirmed in 113 dogs by detection of Babesia canis by microscopic examination of a stained blood smear using the concentration line technique. Results: Thrombocytopenia was present in all 113 dogs. Red blood cell count, packed cell volume and haemoglobin values were below the reference range in 62.8%, 61.1% and 46.0% of affected dogs, respectively. An increased reticulocyte count was apparent in five Babesia canis-positive dogs. Leukopenia, lymphopenia, neutropenia and monocytosis were present in 54.9%, 47.8%, 30.4% and 6.5% of the dogs, respectively. Evaluating haematological parameters by CARTanalysis revealed a predictive model (accuracy = 93.5%) for canine babesiosis, when using the leucocyte, thrombocyte, and reticulocyte count. Climatic conditions present at the most probable time of Babesia canis-infection accounted for biseasonal occurrence. Changes of climatic factors during the year influence the vector activity and in conclusion should highlight babesiosis in the ranking of differentials for veterinarians. Conclusion: The results demonstrate that a tentative diagnosis of canine babesiosis can be made based on typical haematological changes. The results recorded match well with the seasonality of the tick vector and were confirmed here by the month of sample submission.

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