scholarly journals Utility assessment of an Enzyme‐linked immunosorbent assay for detection of subclinical cases of caseous lymphadenitis in small ruminant flocks

2020 ◽  
Vol 6 (4) ◽  
pp. 796-803
Author(s):  
Lina Costa ◽  
Belén Huerta ◽  
Ángela Galán‐Relaño ◽  
Lídia Gómez‐Gascón ◽  
Anabela Almeida ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Small Ruminant ◽  
Enzyme Linked Immunosorbent Assay ◽  
Caseous Lymphadenitis ◽  
Utility Assessment

scholarly journals Etudes de terrain sur la fièvre catarrhale ovine et la maladie hémorragique épizootique en Turquie

2009 ◽  
Vol 62 (2-4) ◽  
pp. 110
Author(s):  
A. Ertürk ◽  
S. G. Cizmeci ◽  
M. F. Barut ◽  
Alberto Allepuz ◽  
Anna Alba ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Mediterranean Region ◽  
Census Data ◽  
Small Ruminant ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lower Prevalence ◽  
High Seroprevalence ◽  
Serum Samples ◽  
Preliminary Results ◽  
The Village

The objective of this study was to detect the presence of new serotypes of bluetongue (BT) circulating in Turkey. A cross-sec­tional study was conducted between March and May 2008 in the provinces of Adana, Mersin, Antalya, Osmaniye, and Hatay (Mediterranean region). The serosurvey was only performed in cattle. This species was not vaccinated and the presence of an­tibodies would indicate that the animal had been infected by field strains of BT virus. Only animals born after the outbreaks were sampled. The epidemiological unit was the village, which was the lowest level for which census data was available. Ran­dom sampling was carried out in 146 villages and eight sam­ples were collected in each village.  A competitive enzyme-linked immunosorbent assay (cELISA) was used for detection of BT antibodies. Preliminary results of the study were presented. From a total of 1096 serum samples collected, 352 (32.1%) were positive to BT. The seroprevalence by province ranged from 15% in Antalya (45/257) to 88% in Osmaniye (77/88). In animals under two years old, the sero­prevalence was 25%, whereas in adult animals it was 52%. The differences in the seroprevalence detected between the five provinces could be attributed to the different density of vector, cattle and small ruminant populations. The high seroprevalence in unvaccinated cattle indicated that BT infection was wide­spread in the ruminant population of Turkey. The young animals presented a lower prevalence than the adults, suggesting that the adults had probably been exposed to the virus before the last two years, whereas the presence of positive seroconversion in young animals indicated that BT virus had been circulating in the two-year period.

Plasminogen Interactions with Immobilized Fibrinogen

1989 ◽  
Vol 62 (04) ◽  
pp. 1078-1082 ◽  
Author(s):  
Burt Adelman ◽  
Patricia Ouynn
Keyword(s):  
Immunosorbent Assay ◽  
Aminocaproic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Regions ◽  
Dose Dependent Fashion ◽  
Epsilon Aminocaproic Acid ◽  
Dose Dependent

SummaryThis report describes the binding of plasminogen to fibrinogen adsorbed onto polystyrene wells. Binding was determined by enzyme linked immunosorbent assay. Both glu- and lys-plasminogen bound to immobilized fibrinogen in a dose-dependent fashion. However, more lys- than glu-plasminogen bound when equal concentrations of either were added to immobilized fibrinogen. Plasminogen binding was inhibited by epsilon aminocaproic acid indicating that binding was mediated via lysine-binding regions of plasminogen. Soluble fibrinogen added in excess of immobilized fibrinogen did not compete for plasminogen binding but fibrinogen fragments produced by plasmin digestion of fibrinogen did. Treatment of immobilized fibrinogen with thrombin caused a small but significant (p <0.01) increase in plasminogen binding. These studies demonstrate that immobilized fibrinogen binds both glu- and lys-plasminogen and that binding is mediated via lysine-binding regions. These interactions may facilitate plasminogen binding to fibrinogen adsorbed on to surfaces and to cells such as platelets which bind fibrinogen.

Alterations in Vascular Endothelial Cell-related Plasma Proteins In Thalassaemic Patients and their Correlation with Clinical Symptoms

1995 ◽  
Vol 74 (04) ◽  
pp. 1045-1049 ◽  
Author(s):  
P Butthep ◽  
A Bunyaratvej ◽  
Y Funahara ◽  
H Kitaguchi ◽  
S Fucharoen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Immunosorbent Assay ◽  
Plasma Proteins ◽  
Clinical Symptoms ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vascular Endothelial ◽  
Leg Ulcers

SummaryAn increased level of plasma thrombomodulin (TM) in α- and β- thalassaemia was demonstrated using an enzyme-linked immunosorbent assay (ELISA). Nonsplenectomized patients with β-thalassaemia/ haemoglobin E (BE) had higher levels of TM than splenectomized cases (BE-S). Patients with leg ulcers (BE-LU) were found to have the highest increase in TM level. Appearance of larger platelets in all types of thalassaemic blood was observed indicating an increase in the number of younger platelets. These data indicate that injury of vascular endothelial cells is present in thalassaemic patients.

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