scholarly journals Ovine pulmonary adenocarcinoma in ewes and lambs in Northern Ireland

2021 ◽  
Vol 188 (5) ◽  
Keyword(s):  
Northern Ireland ◽  
Pulmonary Adenocarcinoma ◽  
Ovine Pulmonary Adenocarcinoma

scholarly journals Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

2016 ◽  
Vol 60 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Aliasghar Bahari ◽  
Masoud Sabouri Ghannad ◽  
Omid Dezfoulian ◽  
Fereydon Rezazadeh ◽  
Ali Sadeghi-Nasab
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Mediastinal Lymph Node ◽  
Pulmonary Adenocarcinoma ◽  
Ovine Pulmonary Adenocarcinoma ◽  
Jaagsiekte Sheep Retrovirus ◽  
Tissue Samples ◽  
Blood Samples ◽  
Proviral Dna ◽  
Healthy Sheep

Abstract Introduction: The aim of this study was to use TaqMan real-time PCR technique to investigate Jaagsiekte sheep retrovirus (JSRV) proviral DNA in whole blood samples of sheep, and compare the results to those of histopathological examinations. Material and Methods: Eighty blood samples from clinically healthy sheep were randomly collected before the animals were slaughtered. Ten tissue samples from each lung and associated caudal mediastinal lymph node were taken. Results: Fifteen (18.75%) blood samples were found to contain proviral DNA, and 11 (13.75%) corresponding lung samples showed microscopic changes consistent with ovine pulmonary adenocarcinoma. None of the samples displayed metastases to the caudal mediastinal lymph nodes. The prominent pattern of neoplastic nodules consisted of acinar (alveolar) form. Conclusion: The results indicated the higher sensitivity of real-time PCR compared to histopathological examinations in detection of ovine pulmonary adenocarcinoma.

Microsatellites in immune-relevant regions and their associations with Maedi-Visna and ovine pulmonary adenocarcinoma viral diseases

2012 ◽  
Vol 145 (1-2) ◽  
pp. 438-446 ◽  
Author(s):  
A. Larruskain ◽  
E. Minguijón ◽  
I. Arostegui ◽  
B. Moreno ◽  
R.A. Juste ◽  
...  
Keyword(s):  
Pulmonary Adenocarcinoma ◽  
Viral Diseases ◽  
Ovine Pulmonary Adenocarcinoma

Overexpression of aldolase A and cytokeratin 19 in ovine pulmonary adenocarcinoma

2012 ◽  
Vol 15 (4) ◽  
pp. 703-709 ◽  
Author(s):  
A. Kycko ◽  
M. Reichert
Keyword(s):  
Epithelial Cells ◽  
Pulmonary Adenocarcinoma ◽  
Cytokeratin 19 ◽  
Immunohistochemical Examination ◽  
Neoplastic Cells ◽  
Ovine Pulmonary Adenocarcinoma ◽  
Immunohistochemical Studies ◽  
Aldolase A ◽  
Experimental Group ◽  
Dimensional Electrophoresis

Abstract Ovine pulmonary adenocarcinoma (OPA) is a transmissible lung cancer of sheep caused by jaagsiekte sheep retrovirus (JSRV). In the present study the protein profiles of five neoplastic and three non-neoplastic sheep lung tissues were examined for the identification of proteins overexpressed in ovine pulmonary adenocarcinoma. Lung sections of the experimental group of sheep were collected during necropsies for proteomic and immunohistochemical examination. Two dimensional electrophoresis (2DE) was performed using gel strips with immobilized pH gradient 3-10. As a result of 2DE gel analysis 14 spots characterized by over 2-fold higher expression in tumour proteomes were selected for mass spectrometry. In eleven spots more than one polypeptide was identified indicating overlapping of proteins in gels. In two spots demonstrating over 3-fold higher expression in OPA proteomes, single proteins: cytokerarin 19 (CK19) and aldolase A were identified. Immunohistochemical studies revealed that CK19 and aldolase A were expressed in the cytoplasm of epithelial cells of bronchioles in non-neoplastic lung sections, as well as epithelial cells of bronchioles and neoplastic cells in lung sections of OPA affected sheep. The results indicate that the overexpression of the two proteins reflects the presence of neoplastic cells in the lungs of OPA affected sheep.

Ovine pulmonary adenocarcinoma: the story to date

In Practice ◽  
2004 ◽  
Vol 26 (7) ◽  
pp. 387-392 ◽  
Author(s):  
Daniela Salvatori ◽  
Marcelo de las Heras ◽  
Mike Sharp
Keyword(s):  
Pulmonary Adenocarcinoma ◽  
Ovine Pulmonary Adenocarcinoma

Evaluation of the efficacy of clinical diagnosis of ovine pulmonary adenocarcinoma

2008 ◽  
Vol 162 (3) ◽  
pp. 88-89 ◽  
Author(s):  
C. Cousens ◽  
M. Graham ◽  
J. Sales ◽  
M. P. Dagleish
Keyword(s):  
Clinical Diagnosis ◽  
Pulmonary Adenocarcinoma ◽  
Ovine Pulmonary Adenocarcinoma

scholarly journals The Diagnostic Challenges of Ovine Pulmonary Adenocarcinoma

Ruminants ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 58-71
Author(s):  
Hélder Quintas ◽  
Isabel Pires ◽  
Andreia Garcês ◽  
Justina Prada ◽  
Filipe Silva ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Pulmonary Adenocarcinoma ◽  
Diagnostic Methods ◽  
Control Measures ◽  
Economic Losses ◽  
Ovine Pulmonary Adenocarcinoma ◽  
X Ray ◽  
Club Cells ◽  
Pulmonary Adenomatosis ◽  
And Control

Ovine pulmonary adenocarcinoma (OPA), also known as sheep pulmonary adenomatosis and jaagsiekte, is a contagious pulmonary tumor of sheep, characterized by neoplastic proliferation of type II pneumocyte and club cells. OPA is induced by the oncogenic activity of the envelope glycoprotein (Env) of exogenous jaagsiekte sheep retrovirus (JSRV). This disease is associated with significant economic losses in numerous sheep raising countries. The onset of suggestive clinical signs is often late, making difficult the early diagnosis of the disease and timely implementation of control measures on the affected farms. Further, the lack of diagnostic tests that can be performed routinely by veterinary clinicians to accurately assess infected animals (e.g., serological or others) means that the true prevalence at flock level is not known. Imaging diagnostic methods (e.g., ultrasound, X-ray and computed tomography) can be used to support the clinical diagnosis, even in pre-clinical stages in affected flocks. The diagnosis must be confirmed by PCR of nasal excretions or immunohistochemistry and PCR of tumor lesions. No vaccine for OPA has yet been developed. Thus, in this work, we review the main methods of diagnosis of OPA in order to support the clinician in the identification of the disease, avoid underdiagnosis and allow the implementation of suitable measures to prevent and control its spread.

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