scholarly journals Reliability and validity of tissue volume measurement by three-dimensional ultrasound: an experimental model

2007 ◽  
Vol 29 (2) ◽  
pp. 210-214 ◽  
Author(s):  
W. P. Martins ◽  
R. A. Ferriani ◽  
D. A. Barra ◽  
R. M. dos Reis ◽  
M. A. V. Bortolieiro ◽  
...  
1998 ◽  
Vol 7 (5) ◽  
pp. 469-478 ◽  
Author(s):  
Jan P. Stegemann ◽  
John J. O'Neil ◽  
Don T. Nicholson ◽  
Claudy J.-P. Mullon

Accurate and consistent measurement of tissue volume is critical to performing many types of islet research; however, conventional visual determination of isolated islet yields through a microscope is heavily operator dependent. An improved method of islet volume determination using digital image analysis (DIA) was developed to remove operator bias and automate the islet counting process. A series of 140 porcine islet isolations were used to evaluate the DIA method in three separate stages. In Stage 1 ( n = 29 isolations), the conventional and DIA methods were correlated with two other independent islet quantitation methods: insulin extraction, and DNA extraction. It was found that volumes determined by DIA correlated more closely with insulin content and DNA content than did conventionally determined volumes. In Stages 2 and 3 ( n = 54 and 57 isolations, respectively), it was shown that an increase in the number of fields analyzed by DIA did not significantly improve the quality of the correlations. Inclusion of very small tissue (<50 fun in diameter), which is ignored in the conventional protocol affected yields by less than 10% and did not significantly improve the correlation with insulin or DNA content. Quantitation of isolated islet tissue volume using DIA has been shown to be rapid, consistent, and objective. In the laboratory, use of this method as the standard for islet volume measurement will allow more meaningful comparison of experimental results between centers. In the clinic, its use will allow more accurate dosing of transplanted tissue. © 1998 Elsevier Science Inc.


2007 ◽  
Vol 276 (1) ◽  
pp. 95-97 ◽  
Author(s):  
Hélio Antonio Guimarães Filho ◽  
Lavoisier Linhares Dias da Costa ◽  
Edward Araujo Júnior ◽  
Cláudio Rodrigues Pires ◽  
Luciano Marcondes Machado Nardozza ◽  
...  

2003 ◽  
Vol 21 (3) ◽  
pp. 283-291 ◽  
Author(s):  
N. J. Raine-Fenning ◽  
J. S. Clewes ◽  
N. R. Kendall ◽  
A. K. Bunkheila ◽  
B. K. Campbell ◽  
...  

2004 ◽  
Vol 23 (2) ◽  
pp. 247-254 ◽  
Author(s):  
Andrej Lyshchik ◽  
Valentina Drozd ◽  
Susanne Schloegl ◽  
Christoph Reiners

2013 ◽  
Vol 73 (3) ◽  
pp. 544-550 ◽  
Author(s):  
Sovira Tan ◽  
Jianhua Yao ◽  
John A Flynn ◽  
Lawrence Yao ◽  
Michael M Ward

ObjectiveSyndesmophyte growth in ankylosing spondylitis can be difficult to measure using radiographs because of poor visualisation and semiquantitative scoring methods. We developed and tested the reliability and validity of a new computer-based method that fully quantifies syndesmophyte volumes and heights on CT scans.MethodsIn this developmental study, we performed lumbar spine CT scans on 38 patients and used our algorithm to compute syndesmophyte volume and height in four intervertebral disk spaces. To assess reliability, we compared results between two scans performed on the same day in nine patients. To assess validity, we compared computed measures to visual ratings of syndesmophyte volume and height on both CT scans and radiographs by two physician readers.ResultsCoefficients of variation for syndesmophyte volume and height, based on repeat scans, were 2.05% and 2.40%, respectively. Based on Bland–Altman analysis, an increase in syndesmophyte volume of more than 4% or in height of more than 0.20 mm represented a change greater than measurement error. Computed volumes and heights were strongly associated with physician ratings of syndesmophyte volume and height on visual examination of both the CT scans (p<0.0001) and plain radiographs (p<0.002). Syndesmophyte volumes correlated with the Schober test (r=−0.48) and lateral thoracolumbar flexion (r=−0.60).ConclusionsThis new CT-based method that fully quantifies syndesmophytes in three-dimensional space had excellent reliability and face and construct validity. Given its high precision, this method shows promise for longitudinal clinical studies of syndesmophyte development and growth.


2019 ◽  
Vol 10 (2) ◽  
pp. 426-439 ◽  
Author(s):  
Ghazala Khan ◽  
Faiza Khan

PurposeThe purpose of this study is to develop and validate a measurement scale for determining the “Halalness” of restaurants and related behaviour among Muslim consumers in the absence of the halal logo. The study responded to scholarly calls for further research in exploring the consumption practices of Muslims.Design/methodology/approachThe scale was developed and validated using a rigorous methodology recommended in the scale development literature. The study used a total sample of 438 (66 respondents for pilot study, 208 for exploratory survey and 164 for the confirmatory survey) to collect the data. Reliability and validity of the measurement scale was established through average variance extracted, Cronbach’s alpha, composite reliability, critical ratios and discriminant validity.FindingsThe results from the exploratory factor analysis and confirmatory analysis propose a three-dimensional measurement scale with 14 items for establishing the “Halalness” of restaurants and including social servicescape; neighbourhood and behaviour.Research limitations/implicationsThe study was unable to validate a number of cues and behaviour such as the presence of images in the restaurant or consumer responses to the presence of alcohol. The researchers are therefore encouraged to test these cues.Originality/valueThe study addresses a gap in Islamic literature, and it is among the first to provide conceptualisation and empirically validate a scale for measuring the “Halalness” of restaurants. The scale was tested and validated based on samples drawn from two different countries, thereby allowing for generalizability.


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