scholarly journals EP21.01: Confocal microtomography (micro-CT) analysis and three-dimensional (3D) reconstruction of pathological conditions affecting the human fallopian tube (FT)

2018 ◽  
Vol 52 ◽  
pp. 272-273
Author(s):  
P.T. Castro ◽  
O.L. Aranda ◽  
A.P. Matos ◽  
E. Marchiori ◽  
H.D. Alves ◽  
...  
Keyword(s):  
3D Reconstruction ◽  
Fallopian Tube ◽  
Three Dimensional ◽  
Micro Ct ◽  
Human Fallopian Tube ◽  
Pathological Conditions ◽  
Ct Analysis

Micro-CT analysis of two-dimensional and three-dimensional parameters in severely curved roots prepared with 3 instrumentation systems: An in vitro study

2020 ◽  
Vol 9 (2) ◽  
pp. 62
Author(s):  
AntonioMiranda da Cruz-Filho ◽  
LuisEduardo Souza-Flamini ◽  
BrunoMonguilhott Crozeta ◽  
RicardoGariba Silva ◽  
RicardoNovak Savioli ◽  
...  
Keyword(s):  
Three Dimensional ◽  
In Vitro Study ◽  
Vitro Study ◽  
Two Dimensional ◽  
Micro Ct ◽  
Ct Analysis ◽  
Dimensional Parameters

Tuboperitoneal fistula, ectopic pregnancy, and remnants of fallopian tube: a confocal microtomography analysis and 3D reconstruction of human fallopian tube pathologies

2018 ◽  
Vol 32 (18) ◽  
pp. 3082-3087 ◽  
Author(s):  
Pedro Teixeira Castro ◽  
Ana Paula Pinho Matos ◽  
Osvaldo Luiz Aranda ◽  
Edson Marchiori ◽  
Haimon Diniz Lopes Alves ◽  
...  
Keyword(s):  
3D Reconstruction ◽  
Ectopic Pregnancy ◽  
Fallopian Tube ◽  
Human Fallopian Tube

scholarly journals Three-dimensional modeling of the human fallopian tube fimbriae

2015 ◽  
Vol 136 (2) ◽  
pp. 348-354 ◽  
Author(s):  
Sharon L. Eddie ◽  
Suzanne M. Quartuccio ◽  
Jie Zhu ◽  
Jessica A. Shepherd ◽  
Rajul Kothari ◽  
...  
Keyword(s):  
Fallopian Tube ◽  
Three Dimensional ◽  
Three Dimensional Modeling ◽  
Human Fallopian Tube ◽  
Dimensional Modeling

scholarly journals Proportional vascularization along the fallopian tubes and ovarian fimbria: assessment by confocal microtomography

2020 ◽  
Vol 53 (3) ◽  
pp. 161-166
Author(s):  
Pedro Teixeira Castro ◽  
Osvaldo Luiz Aranda ◽  
Edson Marchiori ◽  
Luiz Felipe Bittencourt de Araújo ◽  
Haimon Diniz Lopes Alves ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Fallopian Tube ◽  
Morphometric Analysis ◽  
Room Temperature ◽  
Three Dimensional ◽  
The Other ◽  
Fallopian Tubes ◽  
Micro Ct ◽  
Iodine Staining ◽  
Important Relationship

Abstract Objective: To evaluate and reconstruct three-dimensional images of vascularization along the fallopian tube (FT), as well as to determine its relationship with the ovary and ovarian fimbria, and to quantify the blood vessels along the FT according to its anatomical segments, using confocal microtomography (micro-CT). Materials and Methods: Nine specimens (six FTs and three FTs with ovaries) were fixed in a solution of 10% formalin for > 24 h at room temperature. Iodine staining was performed by soaking the specimens in 10% Lugol’s solution for 24 h. All specimens were evaluated using micro-CT. A morphometric analysis was performed on the reconstructed images to quantify the vascular distribution along the FT. Results: In the FTs evaluated, the density of blood vessels was significantly greater in the fimbrial segments than in the isthmic segments (p < 0.05). The ovarian fimbria was clearly identified, demonstrating the important relationship between these vessels and the FT fimbriae. Conclusion: We believe that the vascularization in the fimbriae is greater than and disproportional that in the other segments of FT, and that the ovarian fimbria plays an important role in the development of that difference.

scholarly journals Digital confocal microscopy allows measurement and three-dimensional multiple spectral reconstruction of Neisseria gonorrhoeae/epithelial cell interactions in the human fallopian tube organ culture model.

1994 ◽  
Vol 42 (3) ◽  
pp. 297-306 ◽  
Author(s):  
G L Gorby
Keyword(s):  
Image Analysis ◽  
Confocal Microscopy ◽  
Neisseria Gonorrhoeae ◽  
Organ Culture ◽  
Fallopian Tube ◽  
Three Dimensional ◽  
Target Tissue ◽  
Cell Nuclei ◽  
Computerized Image Analysis ◽  
Human Fallopian Tube

A strategy for measuring Neisseria gonorrhoeae attachment and invasion in the human Fallopian tube organ culture (FTOC) model via computerized image analysis (CIA) combined with "digital" confocal microscopy (DCM) was tested. DCM on serial image stacks of fluorescent latex beads reduced out-of-focus light propagation in the Z-axis (p < 0.005) and improved the shape factor of lateral three-dimensional reconstructions of the beads (p < 0.001). Sections of tissue infected for 44 hr with piliated, Opa+ gonococci were stained with fluorescein-labeled monoclonal anti-gonococcal antibodies, rhodamine-labeled phalloidin, and Hoechst 33342. Serial images collected at identical focal planes for each fluorochrome were subjected to DCM. Epithelial cytoplasmic regions of interest defined by rhodamine-stained actin were superimposed on the corresponding fluorescein-stained and Hoechst-stained images. Fluorescent objects defined by gray-scale threshold were measured by computerized image analysis using different border treatments to differentiate attached from intracellular gonococci or count cell nuclei. Compared with raw images, measurement of DCM images was less dependent on threshold choice (p < 0.05). DCM augments conventional microscopy in removing out-of-focus light from fluorescent images, in reconstructing three-dimensional images, and in quantitatively differentiating extracellular from intracellular gonococci in a natural target tissue.

Three-dimensional imaging and morphometric analysis of alveolar tissue from microfocal X-ray-computed tomography

2006 ◽  
Vol 291 (3) ◽  
pp. L535-L545 ◽  
Author(s):  
Horst Detlef Litzlbauer ◽  
Christoph Neuhaeuser ◽  
Alexander Moell ◽  
Susanne Greschus ◽  
Andreas Breithecker ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Three Dimensional ◽  
Ct Scans ◽  
Micro Ct ◽  
Tissue Samples ◽  
X Ray ◽  
Ct Analysis ◽  
X Ray Computed ◽  
Mean Linear Intercept ◽  
Alveolar Surface

We evaluated microfocal X-ray-computed tomography (micro-CT) as a method to visualize lung architecture two and three dimensionally and to obtain morphometric data. Inflated porcine lungs were fixed by formaldehyde ventilation. Tissue samples (8-mm diameter, 10-mm height) were stained with osmium tetroxide, and 400 projection images (1,024 × 1,024 pixel) were obtained. Continuous isometric micro-CT scans (voxel size 9 μm) were acquired to reconstruct two- and three-dimensional images. Tissue samples were sectioned (8-μm thickness) for histological analysis. Alveolar surface density and mean linear intercept were assessed by stereology-based morphometry in micro-CT scans and corresponding histological sections. Furthermore, stereology-based morphometry was compared with morphometric semi-automated micro-CT analysis within the same micro-CT scan. Agreement of methods was assessed by regression and Bland-Altman analysis. Comparing histology with micro-CT, alveolar surface densities (35.4 ± 2.4 vs. 33.4 ± 1.9/mm, P < 0.05) showed a correlation ( r = 0.72; P = 0.018) with an agreement of 2 ± 1.6/mm; the mean linear intercept (135.7 ± 14.5 vs. 135.8 ± 15 μm) correlated well ( r = 0.97; P < 0.0001) with an agreement of −0.1 ± 3.4 μm. Semi-automated micro-CT analysis resulted in smaller alveolar surface densities (33.4 ± 1.9 vs. 30.5 ± 1/mm; P < 0.01) with a correlation ( r = 0.70; P = 0.023) and agreement of 2.9 ± 1.4/mm. Non-destructive micro-CT scanning offers the advantage to visualize the spatial tissue architecture of small lung samples two and three dimensionally.

scholarly journals Ten Years of Micro-CT in Dentistry and Maxillofacial Surgery: A Literature Overview

2020 ◽  
Vol 10 (12) ◽  
pp. 4328 ◽  
Author(s):  
Ilaria Campioni ◽  
Raffaella Pecci ◽  
Rossella Bedini
Keyword(s):  
Bone Structure ◽  
Ex Vivo ◽  
Maxillofacial Surgery ◽  
Three Dimensional ◽  
3D Models ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Scientific Publications ◽  
Ct Analysis

Micro-computed tomography (micro-CT) is a consolidated imaging technology allowing non-destructive three-dimensional (3D) qualitative and quantitative analysis by the observation of microstructures with high resolution. This paper aims at delivering a structured overview of literature about studies performed using micro-CT in dentistry and maxillofacial surgery (MFS) by analyzing the entire set of articles to portray the state of the art of the last ten years of scientific publications on the topic. It draws the scenario focusing on biomaterials, in vitro and in/ex vivo applications, bone structure analysis, and tissue engineering. It confirms the relevance of the micro-CT analysis for traditional research applications and mainly in dentistry with respect to MFS. Possible developments are discussed in relation to the use of the micro-CT combined with other, traditional, and not, techniques and technologies, as the elaboration of 3D models based on micro-CT images and emerging numerical methods. Micro-CT results contribute effectively with whose ones obtained from other techniques in an integrated multimethod approach and for multidisciplinary studies, opening new possibilities and potential opportunities for the next decades of developments.

scholarly journals Scalable method for micro-CT analysis enables large scale quantitative characterization of brain lesions and implants

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
David B. Kastner ◽  
Viktor Kharazia ◽  
Rhino Nevers ◽  
Clay Smyth ◽  
Daniela A. Astudillo-Maya ◽  
...  
Keyword(s):  
Brain Structure ◽  
Large Scale ◽  
Three Dimensional ◽  
Ct Imaging ◽  
Nondestructive Method ◽  
Brain Lesions ◽  
Dimensional Structure ◽  
Micro Ct ◽  
Ct Analysis ◽  
Quantitative Analyses

AbstractAnatomic evaluation is an important aspect of many studies in neuroscience; however, it often lacks information about the three-dimensional structure of the brain. Micro-CT imaging provides an excellent, nondestructive, method for the evaluation of brain structure, but current applications to neurophysiological or lesion studies require removal of the skull as well as hazardous chemicals, dehydration, or embedding, limiting their scalability and utility. Here we present a protocol using eosin in combination with bone decalcification to enhance contrast in the tissue and then employ monochromatic and propagation phase-contrast micro-CT imaging to enable the imaging of brain structure with the preservation of the surrounding skull. Instead of relying on descriptive, time-consuming, or subjective methods, we develop simple quantitative analyses to map the locations of recording electrodes and to characterize the presence and extent of hippocampal brain lesions.

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