scholarly journals OC21.01: Whole-genome array as a first-tier cytogenetic test in routine prenatal diagnosis

2017 ◽  
Vol 50 ◽  
pp. 43-43
Author(s):  
M. Hynek ◽  
M. Trkova ◽  
V. Becvarova ◽  
H. Pekova ◽  
R. Mansfeldova ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Whole Genome ◽  
Genome Array

scholarly journals Prenatal diagnosis of fetal multicystic dysplastic kidney via high-resolution whole-genome array

2016 ◽  
Vol 31 (10) ◽  
pp. 1693-1698 ◽  
Author(s):  
Fang Fu ◽  
Feifei Chen ◽  
Ru Li ◽  
Yongling Zhang ◽  
Min Pan ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
High Resolution ◽  
Multicystic Dysplastic Kidney ◽  
Whole Genome ◽  
Dysplastic Kidney ◽  
Genome Array

scholarly journals P20.06: The experience with whole-genome array as a first-line cytogenetic test in invasive prenatal diagnosis

2018 ◽  
Vol 52 ◽  
pp. 192-192
Author(s):  
M. Hynek ◽  
M. Trkova ◽  
V. Becvarova ◽  
S. Brezinova ◽  
D. Smetanova ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Whole Genome ◽  
First Line ◽  
Genome Array

scholarly journals Whole-genome array as a first-line cytogenetic test in prenatal diagnosis

2015 ◽  
Vol 45 (4) ◽  
pp. 363-372 ◽  
Author(s):  
M. I. Srebniak ◽  
D. Van Opstal ◽  
M. Joosten ◽  
K. E. M. Diderich ◽  
F. A. T. de Vries ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Whole Genome ◽  
First Line ◽  
Genome Array

scholarly journals Prenatal diagnosis of chromothripsis, with nine breaks characterized by karyotyping, FISH, microarray and whole-genome sequencing

2015 ◽  
Vol 35 (3) ◽  
pp. 299-301 ◽  
Author(s):  
M. J. Macera ◽  
A. Sobrino ◽  
B. Levy ◽  
V. Jobanputra ◽  
V. Aggarwal ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Whole Genome

OC20.06: Whole-genome array CGH in fetuses with major malformations: a Danish study

2012 ◽  
Vol 40 (S1) ◽  
pp. 41-41
Author(s):  
E. Vestergaard ◽  
R. Christensen ◽  
O. B. Petersen ◽  
I. Vogel
Keyword(s):  
Array Cgh ◽  
Whole Genome ◽  
Genome Array ◽  
Danish Study

scholarly journals Clinical implementation of whole-genome array CGH as a first-tier test in 5080 pre and postnatal cases

2011 ◽  
Vol 4 (1) ◽  
pp. 12 ◽  
Author(s):  
Sang-Jin Park ◽  
Eun Jung ◽  
Ran-Suk Ryu ◽  
Hyun Kang ◽  
Jung-Min Ko ◽  
...  
Keyword(s):  
Array Cgh ◽  
Whole Genome ◽  
Clinical Implementation ◽  
Genome Array

Comparison of Different Sources of Mesenchymal Stem Cells: Palatal versus Lipoaspirated Adipose Tissue

2017 ◽  
Vol 204 (5-6) ◽  
pp. 228-240 ◽  
Author(s):  
Sema S. Hakki ◽  
Gizem Turaç ◽  
S. Buket Bozkurt ◽  
Seyit Ali Kayis ◽  
Erdogan E. Hakki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Real Time ◽  
Osteogenic Potential ◽  
Whole Genome ◽  
Array Analysis ◽  
Female Patients ◽  
Healthy Female ◽  
Genome Array

Objectives: The purpose of this study was to compare the proliferation and differentiation potential of mesenchymal stem cells (MSCs) derived from palatal adipose tissue (PAT) and lipoaspirated adipose tissue (LAT). Materials and Methods: PATs were obtained from 2 healthy female patients undergoing surgery for gingival recession, and LATs were obtained from 2 healthy female patients undergoing plastic surgery. LAT- and PAT-derived MSCs were confirmed by flow cytometry using MSC-specific surface markers. The multilineage differentiation capacity of the MSCs was analyzed. The expression of immunophenotyping, embryonic, and differentiation markers was compared between both MSC lines. The proliferation of PAT- and LAT-MSCs was evaluated using a real-time cell analyzer, and telomerase activity was determined using an ELISA-based TRAP assay. Stem cells isolated from PAT and LAT were analyzed by real-time PCR and whole genome array analysis. Results: The cells isolated from PAT had MSC characteristics. In addition, PAT-MSCs had significantly higher alkaline phosphatase activity and osteogenic potential than LAT-MSCs. Although the proliferation and telomerase activities of LAT-MSCs were higher than those of PAT-MSCs, the difference was not statistically significant. The level of embryonic stem cell markers (Oct4 and Nanog) was higher in LAT-MSCs than in PAT-MSCs. The whole genome array analysis demonstrated that 255 gene sequences were differentially expressed, with more than a twofold change in expression. Conclusions: This is the first comparative analysis of the isolation and characterization of MSCs from PAT and LAT. PAT is an accessible source of MSCs, which could be used in periodontal and craniofacial tissue engineering.

Clinical Application of Whole Genome Array Improves the Diagnosis of Pediatric Brain Tumors

2017 ◽  
Vol 25 (8) ◽  
pp. 688-695 ◽  
Author(s):  
Lina Shao ◽  
Sue Miller ◽  
Carl Koschmann ◽  
Sandra Camelo-Piragua
Keyword(s):  
High Resolution ◽  
Brain Tumors ◽  
Copy Number ◽  
Essential Role ◽  
Pediatric Brain Tumors ◽  
Whole Genome ◽  
Diagnosis And Prognosis ◽  
Genetic Abnormalities ◽  
Genome Array ◽  
Pediatric Brain

Pediatric brain tumors are the leading cause of childhood cancer mortality. Recurring genetic abnormalities play an essential role in the diagnosis and prognosis of pediatric brain tumors. However, clinical workup has not routinely included whole genome assessment. Here, we present high resolution whole genome array results in 11 pediatric brain tumors. Array identified clinically relevant abnormalities in all samples. Copy number aberrations with targeted therapy implication, GOPC-ROS1 fusion, CDK4 amplification, and NF1 deletion, were detected in 3 cases. In addition, array detected recurring genetic abnormalities, including KIAA1549-BRAF fusion, 19q13.42 amplification, i(17q), and monosomy 6, which assisted accurate histological diagnosis in pediatric brain tumors. In conclusion, our results show that whole genome high-resolution array detects diagnostic and treatment-relevant copy number abnormalities in pediatric brain tumors.

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