Umbilical vein vasomotion detectedin vivoby serial three-dimensional pixelwise spatially angle-corrected volume flow measurements

2016 ◽  
Vol 47 (5) ◽  
pp. 623-628 ◽  
Author(s):  
T. Scholbach ◽  
C. Heien ◽  
T. M. Eggebø
Keyword(s):  
Umbilical Vein ◽  
Three Dimensional ◽  
Volume Flow ◽  
Flow Measurements

Three-Dimensional Volumetric Spatially Angle-Corrected Pixelwise Fetal Flow Volume Measurement

2011 ◽  
Vol 32 (S 02) ◽  
pp. E122-E128 ◽  
Author(s):  
T. Scholbach ◽  
J. Stolle ◽  
J. Scholbach
Keyword(s):  
Umbilical Vein ◽  
Color Doppler ◽  
Three Dimensional ◽  
Volume Measurement ◽  
Data Sets ◽  
Volume Data ◽  
Flow Measurements ◽  
Perfusion Measurement ◽  
Actual Flow ◽  
Relationship Of

Abstract Purpose: Early attempts to calculate fetal global perfusion used 2 D images. The results were not sufficiently reliable. That‘s why RI measurements are still in use despite the fact that they do not reflect the amount of blood passing through the fetus. We present a novel three-dimensional approach to overcome these limitations. Materials and Methods: In 124 singleton pregnancies between the 23rd and 4st gestational week, a three-dimensional color Doppler sonographic record of the umbilical cord was made, resulting in 281 volume data sets. With dedicated software (PixelFlux) the spatial angle of the umbilical vein was calculated and the true global fetal perfusion was calculated from its horizontal transection as the product of the area of all pixels and the spatial angle-corrected velocity. To validate the PixelFlux technique, phantom flow measurements were carried out. Results: Phantom flow measurements revealed a highly significant correlation of actual flow volumes and those measured by the PixelFlux technique (p < 0.001; rPearson = 0.987 – 0.991) with an even higher interobserver correlation (p < 0.001; rPearson = 0.997). We found a significant correlation of fetal volume flow to gestational age and weight (r = 0.529 at spatial angles below 30° to r = 0.724 at spatial angles below 5°) and a significant influence of the spatial angle on this correlation (p = 0.003; r = – 0.865). Conclusion: Spatial angle-corrected global fetal perfusion measurement improves existing approaches to fetal perfusion evaluation, and is feasible, simple and fast. Thus, it can be recommended to explore the relationship of fetal perfusion and disturbances of fetal development.

In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

2017 ◽  
Vol 68 (6) ◽  
pp. 1341-1344
Author(s):  
Grigore Berea ◽  
Gheorghe Gh. Balan ◽  
Vasile Sandru ◽  
Paul Dan Sirbu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Single Cell ◽  
Cell Line ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

scholarly journals Conductive GelMA–Collagen–AgNW Blended Hydrogel for Smart Actuator

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1217
Author(s):  
Jang Ho Ha ◽  
Jae Hyun Lim ◽  
Ji Woon Kim ◽  
Hyeon-Yeol Cho ◽  
Seok Geun Jo ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Umbilical Vein ◽  
Three Dimensional ◽  
Silver Nanowire ◽  
Structural Deformation ◽  
Rheological Analysis ◽  
Sem Image ◽  
Smart Actuator ◽  
Umbilical Vein Endothelial Cells ◽  
Electrical Stimuli

Blended hydrogels play an important role in enhancing the properties (e.g., mechanical properties and conductivity) of hydrogels. In this study, we generated a conductive blended hydrogel, which was achieved by mixing gelatin methacrylate (GelMA) with collagen, and silver nanowire (AgNW). The ratio of GelMA, collagen and AgNW was optimized and was subsequently gelated by ultraviolet light (UV) and heat. The scanning electron microscope (SEM) image of the conductive blended hydrogels showed that collagen and AgNW were present in the GelMA hydrogel. Additionally, rheological analysis indicated that the mechanical properties of the conductive GelMA–collagen–AgNW blended hydrogels improved. Biocompatibility analysis confirmed that the human umbilical vein endothelial cells (HUVECs) encapsulated within the three-dimensional (3D), conductive blended hydrogels were highly viable. Furthermore, we confirmed that the molecule in the conductive blended hydrogel was released by electrical stimuli-mediated structural deformation. Therefore, this conductive GelMA–collagen–AgNW blended hydrogel could be potentially used as a smart actuator for drug delivery applications.

scholarly journals Acoustic Cell Patterning in Hydrogel for Three-Dimensional Cell Network Formation

Micromachines ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 3
Author(s):  
Kyo-in Koo ◽  
Andreas Lenshof ◽  
Le Thi Huong ◽  
Thomas Laurell
Keyword(s):  
Network Formation ◽  
Umbilical Vein ◽  
Three Dimensional ◽  
Extrusion Process ◽  
Fibroblast Cells ◽  
Glass Capillary ◽  
Cell Network ◽  
Significant Difference ◽  
The One ◽  
Umbilical Vein Endothelial Cells

In the field of engineered organ and drug development, three-dimensional network-structured tissue has been a long-sought goal. This paper presents a direct hydrogel extrusion process exposed to an ultrasound standing wave that aligns fibroblast cells to form a network structure. The frequency-shifted (2 MHz to 4 MHz) ultrasound actuation of a 400-micrometer square-shaped glass capillary that was continuously perfused by fibroblast cells suspended in sodium alginate generated a hydrogel string, with the fibroblasts aligned in single or quadruple streams. In the transition from the one-cell stream to the four-cell streams, the aligned fibroblast cells were continuously interconnected in the form of a branch and a junction. The ultrasound-exposed fibroblast cells displayed over 95% viability up to day 10 in culture medium without any significant difference from the unexposed fibroblast cells. This acoustofluidic method will be further applied to create a vascularized network by replacing fibroblast cells with human umbilical vein endothelial cells.

scholarly journals An Improved Transwell Design for Microelectrode Ion-Flux Measurements

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 273
Author(s):  
Boris Buchroithner ◽  
Pavel Spurný ◽  
Sandra Mayr ◽  
Johannes Heitz ◽  
Dmitry Sivun ◽  
...  
Keyword(s):  
Umbilical Vein ◽  
Cell Monolayer ◽  
Three Dimensional ◽  
Cellular Membrane ◽  
Ion Flux ◽  
Direct Access ◽  
Cell Contact ◽  
Culture Dish ◽  
Tissue Culture Dish ◽  
Transwell System

The microelectrode ion flux estimation (MIFE) is a powerful, non-invasive electrophysiological method for cellular membrane transport studies. Usually, the MIFE measurements are performed in a tissue culture dish or directly with tissues (roots, parts of the plants, and cell tissues). Here, we present a transwell system that allows for MIFE measurements on a cell monolayer. We introduce a measurement window in the transwell insert membrane, which provides direct access for the cells to the media in the upper and lower compartment of the transwell system and allows direct cell-to-cell contact coculture. Three-dimensional multiphoton lithography (MPL) was used to construct a 3D grid structure for cell support in the measurement window. The optimal polymer grid constant was found for implementation in transwell MIFE measurements. We showed that human umbilical vein endothelial cells (HUVECs) efficiently grow and maintain their physiological response on top of the polymer structures.

Impact of an Aortic Nitinol Stent Graft on Flow Measurements by Time-resolved Three-dimensional Velocity-encoded MRI

2012 ◽  
Vol 19 (3) ◽  
pp. 274-280 ◽  
Author(s):  
Fabian Rengier ◽  
Michael Delles ◽  
Roland Unterhinninghofen ◽  
Sebastian Ley ◽  
Sasan Partovi ◽  
...  
Keyword(s):  
Stent Graft ◽  
Three Dimensional ◽  
Flow Measurements ◽  
Time Resolved ◽  
Nitinol Stent

Three-dimensional flow measurements in a static mixer

AIChE Journal ◽  
2012 ◽  
Vol 59 (5) ◽  
pp. 1746-1761 ◽  
Author(s):  
R. T. M. Jilisen ◽  
P. R. Bloemen ◽  
M. F. M. Speetjens
Keyword(s):  
Three Dimensional ◽  
Static Mixer ◽  
Three Dimensional Flow ◽  
Flow Measurements ◽  
Dimensional Flow
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