Optimized parameters using Box–Behnken design methodology facilitate enhanced phenol degradation of Bacillus cereus PB1 by immobilization and adsorption

2020 ◽  
Author(s):  
Selvaraj Radhathirumalaiarasu ◽  
Graham Karthikeyan Mahalakshmi
Keyword(s):  
Bacillus Cereus ◽  
Design Methodology ◽  
Phenol Degradation ◽  
Box Behnken Design

scholarly journals Optimization of Insoluble and Soluble Fibres Extraction fromAgave americanaL. Using Response Surface Methodology

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Amine Bouaziz ◽  
Manel Masmoudi ◽  
Amel Kamoun ◽  
Souhail Besbes
Keyword(s):  
Ionic Strength ◽  
Design Methodology ◽  
Extraction Yield ◽  
Agitation Speed ◽  
Optimum Conditions ◽  
Box Behnken Design ◽  
Factors Affecting ◽  
Soluble Fibre ◽  
Experimental Design Methodology ◽  
Significant Factors

Experimental design methodology was used to determine significant factors affecting the extraction yield of soluble and insoluble fibres fromAgave americanaL. and in second time to find optimum conditions leading to the highest yield. Results clearly indicated that the temperature, the powder to water (P/W) ratio, and the agitation speed were the most important factors influencing fibres extraction yield which increased with temperature, P/W ratio, and agitation speed. Ionic strength affected significantly soluble fibre extraction yield and was the most important factor among nonsignificant ones influencing insoluble fibres extraction yield. Then, a Box-Behnken design was carried out to maximise fibres extraction. Selected optimal conditions were temperature: 90°C; P/W ratio: 0.1625; agitation speed: 400 rpm; and ionic strength: 1.5 g/L. These conditions yielded 93.02% and 80.46% of insoluble and soluble fibres, respectively. Concentrates showed high fibres purity and good functional properties.

One-Step Purification of Catechol 2,3-dioxygenase from Bacillus cereus ZL1

2014 ◽  
Vol 955-959 ◽  
pp. 455-458
Author(s):  
Lin Zhou ◽  
Zi Xiong Zhou ◽  
Shuang Zhu
Keyword(s):  
Bacillus Cereus ◽  
Phenol Degradation ◽  
Potassium Phosphate ◽  
Second Step ◽  
Linear Gradient ◽  
Sds Page ◽  
Key Enzyme ◽  
One Step ◽  
Fast Flow ◽  
Gradient Mode

Catechol 2,3-dioxygenase (C23O), the key enzyme catalyzing the second step in the phenol degradation meta-cleavage pathway has been purified to homogeneity by one-step chromatography from bacterial strain Bacillus cereus ZL1. The culture condition was optimized according to the analysis of the biomass and C23O activity, and the separation process was monitored by SDS-PAGE method. The molecular weight of the purified C23O was 37±3KDa and the active enzyme was eluted by potassium phosphate buffer with 400 mmol NaCL using linear gradient mode on DEAE-Sepharose Fast Flow.

Phenol degradation by Bacillus cereus: Pathway and kinetic modeling

2010 ◽  
Vol 101 (14) ◽  
pp. 5501-5507 ◽  
Author(s):  
Aditi Banerjee ◽  
Aloke K. Ghoshal
Keyword(s):  
Bacillus Cereus ◽  
Kinetic Modeling ◽  
Phenol Degradation

scholarly journals Enzymatic bioconversion of feather waste with keratinases of Bacillus cereus PCM 2849

2019 ◽  
Vol 21 (3) ◽  
pp. 53-59 ◽  
Author(s):  
Dominika P. Ciurko ◽  
Wojciech Łaba ◽  
Michał Piegza ◽  
Piotr Juszczyk ◽  
Anna Choińska-Pulit ◽  
...  
Keyword(s):  
Amino Acids ◽  
Bacillus Cereus ◽  
Reaction Temperature ◽  
Process Conditions ◽  
Box Behnken Design ◽  
Chicken Feathers ◽  
Disulphide Bonds ◽  
Branched Chain ◽  
Enzymatic Bioconversion ◽  
Hydrolysis Of

Abstract Enzymatic preparation from culture of keratinolytic Bacillus cereus PCM 2849 was applied for hydrolysis of whole chicken feathers, after sulphitolytic pretreatment. This process was optimized using a three-factor Box-Behnken design, where the effect of substrate concentration, sulphite concentration during pretreatment and reaction temperature was evaluated on the release of amino acids. Obtained results revealed the highest impact of reaction temperature, followed by substrate content and sulphite during pretreatment. Optimal process conditions were established, i.e. temperature 44.4°C, feathers 4.7% and treatment with 25.3 mM sulphite. Amino acid composition of the obtained hydrolysate was analyzed. Glutamic acid (9.21 g·kg−1) and proline were dominant, however significant amount of branched-chain amino acids was also observed. The FTIR analysis of residual substrate revealed the cleavage of disulphide bonds in keratin through the presence of thioester residues. The absence of reduced cysteine residues was confirmed, along with minor changes in proportions of keratin substructures.

scholarly journals Laccase production by Pycnoporus sp. W-9 using rose dregs and its application for phenol degradation

BioResources ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. 2621-2629
Author(s):  
Xiaohui Liang ◽  
Dongliang Hua ◽  
Yuxiao Zhao ◽  
Hongyu Si ◽  
Bing Wang
Keyword(s):  
Phenol Degradation ◽  
Tween 80 ◽  
Laccase Production ◽  
Phenol Removal ◽  
Optimal Conditions ◽  
Disodium Hydrogen Phosphate ◽  
Box Behnken Design ◽  
Original Production ◽  
Triton X ◽  
Central Composite

Rose dregs were used for laccase production by the strain Pycnoporus sp. W-9 under liquid fermentation, and the obtained laccase was used for phenol degradation. The conditions for laccase production were optimized by Box-Behnken design, and the phenol degradation conditions using the crude laccase were optimized by central composite design. The optimal conditions for laccase production by the strain W-9 were as follows: rose dregs (40 g/L), KCl (0.144 g/L), KH2PO4 (1 g/L), K2HPO4 (0.699 g/L), NaCl (0.2 g/L), MgSO4·7H2O (0.3 g/L), CuSO4·5H2O (0.306 g/L), Tween 80 (0.2 g/L), and CaCO3 (1 g/L), at a pH of 6.0, incubated at 30 °C and 200 rpm for 7 d. The corresponding laccase yield reached 17.4 U/mL, which was approximately 1.8 times the original production. The optimal conditions for phenol removal by the crude laccase were as follows: laccase (12.2 U/mL), 0.1 mol/L citric acid-disodium hydrogen phosphate buffer (pH 3.5), phenol (100 μg/mL), and Triton X-100 (284 μg/mL), incubated at 45 °C for 2 h. The corresponding phenol degradation rate reached 86.6%. These results should be useful for utilization of rose dregs and bioremediation of soil and wastewater.

Box-Behnken design in the development of optimized complex medium for phenol degradation using

1999 ◽  
Vol 21 (5) ◽  
pp. 415
Author(s):  
G. Annadurai ◽  
S. Mathalai Balan ◽  
T. Murugesan
Keyword(s):  
Phenol Degradation ◽  
Complex Medium ◽  
Box Behnken Design

Response surface optimization of cotton tufts opening degree using Box–Behnken designs

2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Weaam Jameel Alkhateeb
Keyword(s):  
Process Parameters ◽  
Design Methodology ◽  
Optimization Procedure ◽  
Laser Method ◽  
Box Behnken Design ◽  
Content Type ◽  
Response Surface Optimization ◽  
The Individual ◽  
One Machine

PurposeThis study aims to investigate the effect of process parameters of blow room machines on openness degree and quality of cotton tufts in a blow room.Design/methodology/approachFor this purpose, an experimental Box–Behnken design (BBD) was used, and the process parameters were the angles of the grid bars underneath the opening rollers of CVT3 beaters and the distance between feed roller of the first opening roller of CVT3.FindingsIt was found that the cotton tuft openness increased by increasing the angles of grid bars and by decreasing the distance between the feed roller and first opening roller on CVT3 beater. Further, the optimization procedure showed that an optimum value of cotton tuft openness (in laser method) was determined for specific levels of the process parameters.Originality/valueThe originality of this investigation is that it showed the individual effects and interactions of the most important factors in two tufting machines instead of only one machine. This study is important because it helps cotton yarn spinners to improve the quality of the final yarns by optimizing the levels of tuft openness which in turn improves fiber cleaning.

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