scholarly journals Initial responses of the trap‐crop, Solanum sisymbriifolium , to Globodera pallida invasions

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Alexander Q. Wixom ◽  
N. Carol Casavant ◽  
Timothy J. Sonnen ◽  
Joseph C. Kuhl ◽  
Fangming Xiao ◽  
...  
Keyword(s):  
Globodera Pallida ◽  
Trap Crop ◽  
Solanum Sisymbriifolium

scholarly journals Assessment of an organ-specific de novo transcriptome of the nematode trap-crop, Solanum sisymbriifolium

2018 ◽  
Author(s):  
Alexander Q Wixom ◽  
N Carol Casavant ◽  
Joseph C Kuhl ◽  
Fangming Xiao ◽  
Louise-Marie Dandurand ◽  
...  
Keyword(s):  
Single Molecule ◽  
De Novo ◽  
Globodera Pallida ◽  
Smrt Sequencing ◽  
Trap Crop ◽  
Site Analysis ◽  
Organ Specific ◽  
Disproportionate Number ◽  
Single Time Point ◽  
Solanum Sisymbriifolium

AbstractSolanum sisymbriifolium, also known as “Litchi Tomato” or “Sticky Nightshade,” is an undomesticated and poorly researched plant related to potato and tomato. Unlike the latter species, S. sisymbriifolium induces eggs of the cyst nematode, Globodera pallida, to hatch and migrate into its roots, but then arrests further nematode maturation. In order to provide researchers with a partial blueprint of its genetic make-up so that the mechanism of this response might be identified, we used single molecule real time (SMRT) sequencing to compile a high quality de novo transcriptome of 41,189 unigenes drawn from individually sequenced bud, root, stem, and leaf RNA populations. Functional annotation and BUSCO analysis showed that this transcriptome was surprisingly complete, even though it represented genes expressed at a single time point. By sequencing the 4 organ libraries separately, we found we could get a reliable snapshot of transcript distributions in each organ. A divergent site analysis of the merged transcriptome indicated that this species might have undergone a recent genome duplication and re-diploidization. Further analysis indicated that the plant then retained a disproportionate number of genes associated with photosynthesis and amino acid metabolism in comparison to genes with characteristics of R-proteins or involved in secondary metabolism. The former processes may have given S. sisymbriifolium a bigger competitive advantage than the latter did.

scholarly journals Evaluation of Solanum sisymbriifolium as a Potential Inoculum Source of Verticillium dahliae and Colletotrichum coccodes

Plant Disease ◽  
2017 ◽  
Vol 101 (7) ◽  
pp. 1300-1305 ◽  
Author(s):  
Z. A. Frederick ◽  
T. F. Cummings ◽  
C. R. Brown ◽  
R. A. Quick ◽  
D. A. Johnson
Keyword(s):  
Verticillium Dahliae ◽  
Potato Cultivar ◽  
Potato Production ◽  
Globodera Pallida ◽  
Colletotrichum Coccodes ◽  
Herbaceous Plant ◽  
Trap Crop ◽  
Inoculum Source ◽  
Russet Norkotah ◽  
Solanum Sisymbriifolium

Solanum sisymbriifolium, the litchi tomato, is a perennial herbaceous plant from South America that is used as a trap crop to reduce soilborne populations of the pale cyst nematode Globodera pallida, an important potato pathogen. Possible interactions of soilborne potato pathogens Verticillium dahliae and Colletotrichum coccodes with litchi tomato are unknown, yet important for potato production if litchi tomato is to be planted as a trap crop. The goal of this research was to quantitatively assess if litchi tomato is a potential inoculum source for C. coccodes and V. dahliae by comparing colony forming units (CFU) observed in litchi tomato to susceptible and resistant potato cultivars. The potato cvs. Alturas (P = 0.0003), Ranger Russet (P = 0.0193), and Russet Norkotah (P = 0.0022) produced more CFUs of the potato pathotype of V. dahliae than litchi tomato the first of two years of greenhouse trials. Significantly more CFUs of the potato pathotype of V. dahliae were quantified from stems and roots of only cv. Russet Norkotah compared with litchi tomato (P = 0.0001) in the second year. The CFUs for C. coccodes varied between litchi tomato and the potato cvs., perhaps due to varying levels of resistance since litchi tomato is from a selected intermated seed source. Based on these data, the effect of litchi tomato in rotation with potato is likely to have limited effect on the proliferation of V. dahliae or C. coccodes populations in the soil when compared with a susceptible potato cultivar.

Effect of steroidal glycoalkaloids on hatch and reproduction of the potato cyst nematode Globodera pallida

Plant Disease ◽  
2021 ◽  
Author(s):  
Syamkumar Sivasankara Pillai ◽  
Louise-Marie Dandurand
Keyword(s):  
Chemical Defense ◽  
Deleterious Effect ◽  
Cyst Nematode ◽  
Russet Burbank ◽  
Globodera Pallida ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
First Line ◽  
Trap Crop ◽  
Solanum Sisymbriifolium

Steroidal glycoalkaloids (SGAs) are phytoanticipins found in solanaceous crops that act as the first line of chemical defense against pathogen attacks. Solanum sisymbriifolium, a trap crop for potato cyst nematodes, has been shown to effectively reduce populations of Globodera pallida. Solanum sisymbriifolium contains α- solamargine and other solasodine type glycoalkaloids that may contribute to plant defenses. The current study evaluated the influence of solanaceous SGAs on G. pallida hatch, development, and reproduction. Exposure to α- solamargine and α- solamarine reduced G. pallida hatch by 65 % and 87 % respectively. Exposure of G. pallida cysts with the glycoalkaloids α- solamargine and solasodine significantly reduced infection in susceptible potato Russet Burbank by 98 and 94 % compared to the control. Exposure of cysts to either solasodine or solamargine significantly reduced reproduction of G. pallida on Russet Burbank by 99 % compared to the control. The study demonstrated the deleterious effect of SGAs on G. pallida hatch, infection, and reproduction.

Evaluation of trap crop, Solanum sisymbriifolium and antagonistic crops against potato cyst nematodes, Globodera spp.

2021 ◽  
Vol 138 ◽  
pp. 242-248
Author(s):  
Priyank Hanuman Mhatre ◽  
K.L. Divya ◽  
E.P. Venkatasalam ◽  
Aarti Bairwa ◽  
R. Sudha ◽  
...  
Keyword(s):  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Trap Crop ◽  
Solanum Sisymbriifolium

scholarly journals Transcriptome analysis of Globodera pallida from the susceptible host Solanum tuberosum or the resistant plant Solanum sisymbriifolium

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Rinu Kooliyottil ◽  
Louise-Marie Dandurand ◽  
Joseph C. Kuhl ◽  
Allan Caplan ◽  
Fangming Xiao ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Sex Differentiation ◽  
Globodera Pallida ◽  
Peptide Sequence ◽  
Secretory Proteins ◽  
Trap Crop ◽  
Uncharacterized Protein ◽  
Susceptible Host ◽  
Signal Peptide Sequence ◽  
Effector Genes

Abstract A transcriptome analysis of G. pallida juveniles collected from S. tuberosum or S. sisymbriifolium 24 h post infestation was performed to provide insights into the parasitic process of this nematode. A total of 41 G. pallida genes were found to be significantly differentially expressed when parasitizing the two plant species. Among this set, 12 were overexpressed when G. pallida was parasitizing S. tuberosum and 29 were overexpressed when parasitizing S. sisymbriifolium. Out of the 12 genes, three code for secretory proteins; one is homologous to effector gene Rbp-4, the second is an uncharacterized protein with a signal peptide sequence, and the third is an ortholog of a Globodera rostochiensis effector belonging to the 1106 effector family. Other overexpressed genes from G. pallida when parasitizing S. tuberosum were either unknown, associated with a stress or defense response, or associated with sex differentiation. Effector genes namely Eng-1, Cathepsin S-like cysteine protease, cellulase, and two unknown genes with secretory characteristics were over expressed when G. pallida was parasitizing S. sisymbriifolium relative to expression from S. tuberosum. Our findings provide insight into gene regulation of G. pallida while infecting either the trap crop S. sisymbriifolium or the susceptible host, S. tuberosum.

scholarly journals Assessment of an Organ-Specific de Novo Transcriptome of the Nematode Trap-Crop, Solanum sisymbriifolium

2018 ◽  
Vol 8 (7) ◽  
pp. 2135-2143 ◽  
Author(s):  
Alexander Q. Wixom ◽  
N. Carol Casavant ◽  
Joseph C. Kuhl ◽  
Fangming Xiao ◽  
Louise-Marie Dandurand ◽  
...  
Keyword(s):  
De Novo ◽  
De Novo Transcriptome ◽  
Trap Crop ◽  
Organ Specific ◽  
Solanum Sisymbriifolium

Field performance of Solanum sisymbriifolium, a trap crop for potato cyst nematodes. II. Root characteristics

2007 ◽  
Vol 150 (1) ◽  
pp. 99-106 ◽  
Author(s):  
B.G.H. Timmermans ◽  
J. Vos ◽  
T.J. Stomph ◽  
J. Van Nieuwburg ◽  
P.E.L. Van der Putten
Keyword(s):  
Field Performance ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Trap Crop ◽  
Root Characteristics ◽  
Solanum Sisymbriifolium
Sign in / Sign up

Export Citation Format

Share Document