In vitro antitumoral effects of the steroid ouabain on human thyroid papillary carcinoma cell lines

2021 ◽  
Author(s):  
Mariana Pires Teixeira ◽  
Eliza Freitas Passos ◽  
Natalia Ferreira Haddad ◽  
Marcelle Novaes Andrade ◽  
Vivian Mary Rumjanek ◽  
...  
Keyword(s):  
Papillary Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Thyroid Papillary Carcinoma ◽  
Human Thyroid ◽  
Carcinoma Cell Lines ◽  
Thyroid Papillary

scholarly journals Human thyroid carcinoma cell lines show different retinoic acid receptor repertoires and retinoid responses

2004 ◽  
pp. 547-556 ◽  
Author(s):  
C Schmutzler ◽  
C Hoang-Vu ◽  
B Ruger ◽  
J Kohrle
Keyword(s):  
Retinoic Acid ◽  
Thyroid Carcinoma ◽  
Mrna Expression ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Human Thyroid ◽  
Type I ◽  
Carcinoma Cell Lines ◽  
Thyroid Carcinoma Cell

OBJECTIVE: Disturbed expression of retinoic acid (RA) receptors (RAR/RXR) contributes to the pathogenesis and tumor progression of epithelial carcinomas. DESIGN: To examine whether altered responses to retinoids may correlate with differences in RA receptor equipment, retinoid effects were examined in human thyroid carcinoma cell lines of various differentiation stages in culture and after xenotransplantation onto rodent models. METHODS: Cell growth was assessed by the MTT test, mRNA expression was examined by Northern blot and quantitative competitive RT-PCR, and type I 5'-deiodinase (5'DI) activity was measured by in vitro deiodination assay. Nude rats and mice were used for xenotransplantation experiments. RESULTS: All-trans-RA and RAR-selective synthetic retinoids stimulated activity and mRNA expression of the thyroid differentiation marker 5'DI in the follicular thyroid carcinoma cell line FTC-133. In the less differentiated FTC-238 cells, stimulation of 5'DI activity was less pronounced than in FTC-133 cells, and a reduced level of RAR beta mRNA was detected. In the anaplastic thyroid carcinoma cell lines HTh 74 and C 643, the activity of 5'DI was not increased by retinoids, and expression of RAR alpha mRNA was reduced. Proliferation of FTC-133 and FTC-238 cells was decreased by all-trans-RA. Pretreatment of FTC-133 with RA resulted in a reduced tumor growth in xenotransplantation experiments as compared with untreated control cells. This reduction was less pronounced in the case of FTC-238 cells. Thus, retinoid therapy might be applied to treat follicular thyroid carcinomas. However, tumor-specific RAR repertoires need to be analyzed as a prerequisite for successful intervention with appropriate, probably receptor-selective retinoids.

Suppression of growth in vitro and tumorigenicity in vivo of human carcinoma cell lines by transfectedp16INK4

1996 ◽  
Vol 16 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Elisa A. Spillare ◽  
Aikou Okamoto ◽  
Koichi Hagiwara ◽  
Douglas J. Demetrick ◽  
Manuel Serrano ◽  
...  
Keyword(s):  
Cell Lines ◽  
Carcinoma Cell ◽  
Human Carcinoma ◽  
Carcinoma Cell Lines ◽  
Human Carcinoma Cell
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