Endoplasmic reticulum stress contributes to arsenic trioxide-induced intrinsic apoptosis in human umbilical and bone marrow mesenchymal stem cells

2014 ◽  
Vol 31 (3) ◽  
pp. 314-328 ◽  
Author(s):  
Yih-An King ◽  
Yu-Jen Chiu ◽  
Hao-Ping Chen ◽  
Daih-Huang Kuo ◽  
Chi-Cheng Lu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Endoplasmic Reticulum ◽  
Mesenchymal Stem Cells ◽  
Endoplasmic Reticulum Stress ◽  
Arsenic Trioxide ◽  
Intrinsic Apoptosis ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Induction of Apoptosis Coupled to Endoplasmic Reticulum Stress through Regulation of CHOP and JNK in Bone Marrow Mesenchymal Stem Cells from Patients with Systemic Lupus Erythematosus

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Genkai Guo ◽  
Yan Meng ◽  
Wei Tan ◽  
Yunfei Xia ◽  
Chun Cheng ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Stem Cells ◽  
Bone Marrow ◽  
Endoplasmic Reticulum ◽  
Mesenchymal Stem Cells ◽  
Endoplasmic Reticulum Stress ◽  
Lupus Erythematosus ◽  
B Cell Lymphoma ◽  
Systemic Lupus ◽  
Marrow Mesenchymal Stem Cells

Previous studies indicated that bone marrow mesenchymal stem cells (BM-MSCs) from patients with systemic lupus erythematosus (SLE) exhibited the phenomenon of apoptosis. In this study, we aimed to investigate whether apoptosis of BM-MSCs from SLE patients were dysregulated. In this paper, endoplasmic reticulum stress (ERS) was evidenced by increased expression of phosphorylated protein kinase RNA-like ER kinase (PERK) and inositol-requiring protein-1 (IRE-1). We also found the activation of downstream target eukaryotic translation initiator factor 2α(eIF 2α) and CCAAT/enhancer-binding protein- (C/EBP-) homologous protein (CHOP) in BM-MSCs from SLE patients. Interestingly, we discovered that 4-phenylbutyric acid (4-PBA), a selective inhibitor of ERS, blocked the apoptosis of BM-MSCs from SLE patients and alleviated the level of Jun N-terminal kinase1/2 (JNK1/2) and CHOP. Furthermore, blockage of PERK signaling expression by siRNA not only significantly reduced the expression of CHOP, but also activated the anti-apoptotic regulator B-cell lymphoma-2 (Bcl-2). Blockage of IRE-1 or JNK1/2 by siRNA resulted in the decreased expression of JNK1/2 and proapoptosis protein Bcl-2 associated protein X (BAX). These results implicated that ERS-mediated apoptosis was a critical determinant of BM-MSCs from SLE patients.

scholarly journals Research Progress on the Role of Endoplasmic Reticulum Stress in Osteogenic Differentiation

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Nianqiang Jin ◽  
Hongchen Sun
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Osteogenic Differentiation ◽  
Treatment Strategies ◽  
Research Progress ◽  
Main Site ◽  
Marrow Mesenchymal Stem Cells

The endoplasmic reticulum (ER) is the main site for regulating protein synthesis and processing. Endoplasmic reticulum stress plays a role in regulating the osteogenic differentiation of stem cells and general osteoblasts. Bone marrow stromal cells (BMSCs, also known as bone marrow mesenchymal stem cells) are a group of progenitor cells that contain a small number of bone stem cells (SSCs) that rebuild cartilage, bone, stroma, and fat cells that support hematopoiesis and bone marrow. Therefore, due to their self-renewal and differentiation capabilities, they have become an important resource for researching regenerative medicine and tissue engineering treatment strategies. Exposure of osteoblasts to physical and biochemical stimuli facilitates rapid activation of early tissue repair processes in organisms. Therefore, the rational regulation of the induction conditions of osteoblasts has become a hot research topic. This article reviews the recent advances in the role of endoplasmic reticulum stress in the process of osteoblast differentiation.

In vitro effect of prolactin on the osteogenic potential of bone marrow mesenchymal stem cells of rats

2013 ◽  
Author(s):  
Melo Ocarino Natalia de ◽  
Silvia Silva Santos ◽  
Lorena Rocha ◽  
Juneo Freitas ◽  
Reis Amanda Maria Sena ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Potential ◽  
Marrow Mesenchymal Stem Cells ◽  
Vitro Effect

Effect of lactation on the osteogenic potential of bone marrow mesenchymal stem cells of rats

2014 ◽  
Author(s):  
Reis Amanda Maria Sena ◽  
Freitas Silva Juneo de ◽  
Silvia Silva Santos ◽  
Rogeria Serakides ◽  
Melo Ocarino Natalia de
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Potential ◽  
Marrow Mesenchymal Stem Cells
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