scholarly journals Hexavalent chromium-induced multiple biomarker responses in liver and kidney of goldfish, Carassius auratus

2010 ◽  
Vol 26 (6) ◽  
pp. 649-656 ◽  
Author(s):  
Venkatramreddy Velma ◽  
Paul B. Tchounwou
Keyword(s):  
Hexavalent Chromium ◽  
Carassius Auratus ◽  
Goldfish Carassius Auratus ◽  
Liver And Kidney

Cobalt-induced oxidative stress in brain, liver and kidney of goldfish Carassius auratus

Chemosphere ◽  
2011 ◽  
Vol 85 (6) ◽  
pp. 983-989 ◽  
Author(s):  
Olha I. Kubrak ◽  
Viktor V. Husak ◽  
Bohdana M. Rovenko ◽  
Janet M. Storey ◽  
Kenneth B. Storey ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carassius Auratus ◽  
Goldfish Carassius Auratus ◽  
Liver And Kidney

scholarly journals Oxidative Stress and DNA Damage Induced by Chromium in Liver and Kidney of Goldfish, Carassius auratus

2013 ◽  
Vol 8 ◽  
pp. BMI.S11456 ◽  
Author(s):  
Venkatramreddy Velma ◽  
Paul B. Tchounwou
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Carassius Auratus ◽  
Industrial Effluents ◽  
Lipid Hydroperoxides ◽  
Kidney Cells ◽  
Surrounding Water ◽  
Goldfish Carassius Auratus ◽  
Liver And Kidney ◽  
Abundant Element

Chromium (Cr) is an abundant element in the Earth's crust. It exhibits various oxidation states, from divalent to hexavalent forms. Cr has diverse applications in various industrial processes and inadequate treatment of the industrial effluents leads to the contamination of the surrounding water resources. Hexavalent chromium (Cr (VI)) is the most toxic form, and its toxicity has been associated with oxidative stress. The present study was designed to investigate the toxic potential of Cr (VI) in fish. In this research, we investigated the role of oxidative stress in chromium-induced genotoxicity in the liver and kidney cells of goldfish, Carassius auratus. Goldfish were acclimatized to the laboratory conditions and exposed them to 5% and 10% of 96 hr-LC50 (85.7 mg/L) of aqueous Cr (VI) in a continuous flow through system. Fish were sampled every 7 days for a period of 28 days to analyze the lipid hydroperoxides (LHP) levels and genotoxic potentials in the liver and kidney. LHP levels were analyzed by spectrophotometry while genotoxicity was assessed by single cell gel electrophoresis (comet) assay. LHP levels in the liver increased significantly at week 1, followed by a decrease. LHP levels in the kidney increased significantly at weeks 1, 2, and 3, and decreased at week 4 compared to the control. The percentage of DNA damage increased in both liver and kidney at both test concentrations. The results clearly indicate that Cr (VI) induces significant levels of DNA damage in liver and kidney cells of goldfish. The induced LHP levels in both organs were concentration-dependent and were directly correlated with the levels of DNA damage. The two tested Cr (VI) concentrations induced significant levels of oxidative stress in both organs, however the kidney appears to be more vulnerable and sensitive to Cr-induced toxicity than the liver.

Acute Toxicity of Sodium Chloride, Pentachlorophenol, Guthion®, and Hexavalent Chromium to Fathead Minnows (Pimephales promelas) and Goldfish (Carassius auratus)

1976 ◽  
Vol 33 (2) ◽  
pp. 203-208 ◽  
Author(s):  
Ira R. Adelman ◽  
Lloyd L. Smith Jr. ◽  
Gary D. Siesennop
Keyword(s):  
Sodium Chloride ◽  
Acute Toxicity ◽  
Hexavalent Chromium ◽  
Carassius Auratus ◽  
Pimephales Promelas ◽  
Fathead Minnows ◽  
Goldfish Carassius Auratus ◽  
Significant Difference

The 96-h LC50’s for sodium chloride were 7650 and 7341 mg/liter, for pentachlorophenol 0.21 and 0.22 mg/liter, for Guthion® 1.9 and 2.4 mg/liter, and for hexavalent chromium 48 and 120 mg/liter, for fathead minnows (Pimephales promelas) and goldfish (Carassius auratus), respectively. Threshold LC50’s were reached in 6 days for sodium chloride (7650 and 7322 mg/liter for fathead minnows and goldfish, respectively), and pentachlorophenol (0.21 and 0.21 mg/liter), but were not attained in 11 days (termination of testing) with Guthion® (0.76 and 0.80 mg/liter) and hexavalent chromium (18 and 33 mg/liter). With pentachlorophenol and Guthion® goldfish were initially more resistant, but by termination there was no significant difference in LC50’s between the two species. With hexavalent chromium the goldfish were more resistant throughout the 11-day test, and with sodium chloride goldfish were initially more resistant but at attainment of a threshold LC50 were less resistant. Use of toxicity curves for assessment of acute mortality permits interpretation not possible in 96-h tests where LC50’s are computed at 24-h intervals.

SEM of Hepatocytes and Biliary Passages in Goldfish Liver

1977 ◽  
Vol 35 ◽  
pp. 556-557
Author(s):  
Waykin Nopanitaya ◽  
Joe W. Grisham ◽  
Johnny L. Carson
Keyword(s):  
Carassius Auratus ◽  
Cell Layer ◽  
Three Dimensional ◽  
Cell Types ◽  
Biliary System ◽  
Fish Food ◽  
Commercial Fish ◽  
Goldfish Carassius Auratus ◽  
Commercial Fish Food

An interesting feature of the goldfish liver is the morphology of the hepatic plate, which is always formed by a two-cell layer of hepatocytes. Hepatic plates of the goldfish liver contain an infrequently seen second type of cell, in the centers of plates between two hepatocytes. A TEH study by Yamamoto (1) demonstrated ultrastructural differences between hepatocytes and centrally located cells in hepatic plates; the latter were classified as ductule cells of the biliary system. None of the previous studies clearly showed a three-dimensional organization of the two cell types described. In the present investigation we utilize SEM to elucidate the arrangement of hepatocytes and bile ductular cells in intralobular plates of goldfish liver.Livers from young goldfish (Carassius auratus), about 6-10 cm, fed commercial fish food were used for this study. Hepatic samples were fixed in 4% buffered paraformaldehyde, cut into pieces, fractured, osmicated, CPD, mounted Au-Pd coated, and viewed by SEM at 17-20 kV. Our observations were confined to the ultrastructure of biliary passages within intralobular plates, ductule cells, and hepatocytes.

Induction of Gynogenesis and Androgenesis in Goldfish Carassius auratus (var. oranda)

2001 ◽  
Vol 36 (3-4) ◽  
pp. 195-198 ◽  
Author(s):  
I Paschos ◽  
L Natsis ◽  
C Nathanailides ◽  
I Kagalou ◽  
E Kolettas
Keyword(s):  
Carassius Auratus ◽  
Goldfish Carassius Auratus
Sign in / Sign up

Export Citation Format

Share Document