Histological alterations in the prolactin cells of a teleost, Heteropneustes fossilis, after exposure to cypermethrin

2010 ◽  
Vol 26 (4) ◽  
pp. 359-363 ◽  
Author(s):  
Diwakar Mishra ◽  
Rubi Rai ◽  
Sunil K. Srivastav ◽  
Ajai K. Srivastav
2010 ◽  
Vol 92 (10) ◽  
pp. 1857-1864 ◽  
Author(s):  
Ajai Kumar Srivastav ◽  
Sanjay Kumar Srivastava ◽  
Diwakar Mishra ◽  
Sunil Kumar Srivastav

2013 ◽  
Vol 39 (2) ◽  
pp. 111-114
Author(s):  
Ajai K. Srivastav ◽  
Rubi Rai ◽  
Nobuo Suzuki ◽  
Diwakar Mishra ◽  
S.K. Srivastav

2019 ◽  
Vol 13 (4) ◽  
pp. 17-20
Author(s):  
Chandra Prakash ◽  
◽  
Sunil Kumar Srivastav ◽  

Background: Previous studies have been reported on the toxicity of Microcystin-LR, which is produced by cyanobacterial growth in fish, such as Heteropneustes fossilis (H. fossilis). However, no studies have been conducted on the effects of Microcystin-LR on the prolactin cells of H. fossilis. Methods: H. fossilis fish were intraperitoneally injected with Microcystin-LR (2.5μg/25g) and sacrificed after 1, 3, 5, 10 and 15 days. The blood samples were analyzed for the calcium levels. Histological slides of the pituitary prolactin cells were stained with Herlant’s tetrachrome and Heidenhan’s azan techniques, and examined under light microscopy. Results: The prolactin cells exposed to Microcystin-LR exhibited no structural changes on day 1. However, hyperactive prolactin cells exhibited cellular degranulation from day 3 to day 5. On days 10 and 15, degenerated and vacuolated prolactin cells were also observed. The nuclear volume of prolactin cells exposed to MC-LR increased progressively from day 3 to day 10 but on day 15 the nuclear volume returned to normal. The serum calcium level of MC-LR injected specimens showed hypocalcemia from day 3 to day 10. On day 15, the level became normal. Conclusions: Our results indicated that the serum calcium levels and prolactin cells were altered after exposure to microcystin-LR.


2012 ◽  
Vol 34 (4) ◽  
Author(s):  
Ajai Kumar Srivastav ◽  
Sanjay Kumar Srivastava ◽  
Sarojni Tripathi ◽  
Diwakar Mishra ◽  
Sunil Kumar Srivastav

Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs

Investigation of the spontaneous pituitary adenomas in rat have been limited mainly to light microscopic study. Furth et al. (1973) described them as chromophobic, secreting prolactin. Kovacs et al. (1977) in an ul trastructural investigation of adenomas of old female Long-Evans rats, found that they were composed of prolactin cells. Berkvens et al. (1980) using immunocytochemistry at the light microscopic level, demonstrated that some spontaneous tumors of old Wistar rats could contain GH, TSH or ACTH as well as PRL.


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