Transportation of transplantable cell sheets fabricated with temperature-responsive culture surfaces for regenerative medicine

2008 ◽  
Vol 2 (4) ◽  
pp. 190-195 ◽  
Author(s):  
Takayuki Nozaki ◽  
Masayuki Yamato ◽  
Toshiaki Inuma ◽  
Kohji Nishida ◽  
Teruo Okano
Keyword(s):  
Regenerative Medicine ◽  
Cell Sheets ◽  
Temperature Responsive ◽  
Transplantable Cell

Transportation of Transplantable Cell Sheets for Regenerative Medicine Treatment

2012 ◽  
Vol 94 (10S) ◽  
pp. 1009
Author(s):  
T. Nozaki ◽  
Y. Oie ◽  
S. Takeda ◽  
K. Mori ◽  
N. Moriya ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Cell Sheets ◽  
Transplantable Cell

Nanotechnology-Based Cell Sheet Engineering for Regenerative Medicine

2006 ◽  
Vol 53 ◽  
pp. 74-78
Author(s):  
Masayuki Yamato ◽  
Teruo Okano
Keyword(s):  
Regenerative Medicine ◽  
Immobilized Cells ◽  
Cell Sheet ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
Intelligent Materials ◽  
Responsive Polymers ◽  
Temperature Responsive ◽  
Novel Technology ◽  
Temperature Responsive Polymers

In order to realize true regenerative medicine, we have developed a novel technology for the reconstruction of tissues and organs by utilizing intelligent materials including temperature-responsive polymers. We developed temperature-responsive culture surfaces, on which temperature-responsive polymers are covalently immobilized. Cells are cultured on the surfaces at 37°C, and harvested as transplantable cell sheets by reducing temperature to 20°C. With these cell sheets we regenerate various kinds of tissues such as cornea and heart.

Validation System of Tissue-Engineered Epithelial Cell Sheets for Corneal Regenerative Medicine

2010 ◽  
Vol 16 (4) ◽  
pp. 553-560 ◽  
Author(s):  
Ryuhei Hayashi ◽  
Masayuki Yamato ◽  
Hiroshi Takayanagi ◽  
Yoshinori Oie ◽  
Akira Kubota ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Regenerative Medicine ◽  
Cell Sheets

Temperature-responsive culture surfaces for insect cell sheets to fabricate a bioactuator

2019 ◽  
Vol 33 (5) ◽  
pp. 219-231 ◽  
Author(s):  
Kaoru Uesugi ◽  
Yui Sakuma ◽  
Yoshitake Akiyama ◽  
Yoshikatsu Akiyama ◽  
Kikuo Iwabuchi ◽  
...  
Keyword(s):  
Insect Cell ◽  
Cell Sheets ◽  
Temperature Responsive

scholarly journals Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering

2009 ◽  
Vol 6 (suppl_3) ◽  
Author(s):  
Kenichi Nagase ◽  
Jun Kobayashi ◽  
Teruo Okano
Keyword(s):  
Cell Sheet ◽  
Copolymer Composition ◽  
Cell Sheets ◽  
Chromatographic Separations ◽  
Cell Sheet Engineering ◽  
External Temperature ◽  
Cell Monolayers ◽  
Temperature Responsive ◽  
Confluent Cell ◽  
Dependent Cell

Temperature-responsive intelligent surfaces, prepared by the modification of an interface with poly( N -isopropylacrylamide) and its derivatives, have been used for biomedical applications. Such surfaces exhibit temperature-responsive hydrophilic/hydrophobic alterations with external temperature changes, which, in turn, result in thermally modulated interactions with biomolecules and cells. In this review, we focus on the application of these intelligent surfaces to chromatographic separation and cell cultures. Chromatographic separations using several types of intelligent surfaces are mentioned briefly, and various effects related to the separation of bioactive compounds are discussed, including wettability, copolymer composition and graft polymer architecture. Similarly, we also summarize temperature-responsive cell culture substrates that allow the recovery of confluent cell monolayers as contiguous living cell sheets for tissue-engineering applications. The key factors in temperature-dependent cell adhesion/detachment control are discussed from the viewpoint of grafting temperature-responsive polymers, and new methodologies for effective cell sheet culturing and the construction of thick tissues are summarized.

scholarly journals Tissue-Engineered Cell Sheets for Stricture Prevention: A New Connection Between Endoscopy and Regenerative Medicine

2012 ◽  
Vol 143 (3) ◽  
pp. 526-529 ◽  
Author(s):  
Joshua D. Penfield ◽  
Emmanuel C. Gorospe ◽  
Kenneth K. Wang
Keyword(s):  
Regenerative Medicine ◽  
Cell Sheets
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