Mesenchymal stromal cells improve the osteogenic capabilities of mineralized agarose gels in a rat full-thickness cranial defect model

2012 ◽  
Vol 7 (1) ◽  
pp. 51-60 ◽  
Author(s):  
Norihiko Mizuta ◽  
Koji Hattori ◽  
Yoshika Suzawa ◽  
Soichi Iwai ◽  
Tomohiro Matsumoto ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Full Thickness ◽  
Defect Model ◽  
Agarose Gels ◽  
Cranial Defect

scholarly journals Ex vivo MRI cell tracking of autologous mesenchymal stromal cells in an ovine osteochondral defect model

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Hareklea Markides ◽  
Karin J. Newell ◽  
Heike Rudorf ◽  
Lia Blokpoel Ferreras ◽  
James E. Dixon ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Cell Tracking ◽  
Osteochondral Defect ◽  
Ex Vivo ◽  
Defect Model

scholarly journals The effect of human adipose-derived multipotent mesenchymal stromal cells in the fibrin gel on the healing of full-thickness skin excision wounds in mice

2017 ◽  
Vol 5 (1) ◽  
pp. 14-22 ◽  
Author(s):  
O. Tykhvynskaya ◽  
O. Rogulska ◽  
N. Volkova ◽  
E. Revenko ◽  
S. Mazur ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Granulation Tissue ◽  
Stromal Cells ◽  
Healing Process ◽  
Complete Recovery ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Scar Tissue ◽  
Human Blood Plasma ◽  
Full Thickness ◽  
Fibrin Gel

Prospects for the widespread use of multipotent mesenchymal stromal cells (MSCs) in regenerative medicine determine the relevance of studying their abilities to affect the reparative process in experimental systems in vivo.Materials and methods. The effect of human adipose-derived MSCs on the healing rate and completeness of damaged skin site reconstitution was examined using full-thickness excision wound model in mice. The reparative activity of MSCs was revealed in planimetric and histological studies. Human blood plasma-derived fibrin gel was used as a scaffold for MSCs delivery.Results and conclusions. Compared to the spontaneous healing process, application of fibrin gel on the excisional skin wounds promotes earlier maturation of granulation tissue and further formation of loose scar tissue with skin derivates. MSCs in the fibrin gel contribute to the improve of wound epithelialization, the decrease of the inflammatory response, faster maturation of the granulation tissue, including marks of angiogenesis, as well as promotes complete recovery of the dermal and epidermal layers of the damaged site of skin.

scholarly journals Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound Repair

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Paola Di Francesco ◽  
Pauline Cajon ◽  
Christophe Desterke ◽  
Marie-France Perron Lepage ◽  
Jean-Jacques Lataillade ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wound Repair ◽  
Positive Impact ◽  
Early Stage ◽  
Full Thickness ◽  
Skin Punch Biopsy ◽  
Significant Difference

Objective. To assess the clinical value and safety of the application of allogeneic equine oral mucosa mesenchymal stromal cells (OM-MSCs) to wounds. Animals. 8 healthy adult horses without front limb skin lesions or musculoskeletal disease. Procedures. Stem cells were isolated from the oral mucosa of a donor horse. Horses were subjected to the creation of eight full-thickness cutaneous wounds, two on each distal forelimb (FL) and two on both sides of the thorax (TH). Each wound was subjected to one out of four treatments: no medication (T1), hyaluronic acid- (HA-) gel containing OM-MSC (T2), HA-gel containing OM-MSC secretome (T3), and HA-gel alone (T4). Gross macroscopic evaluation and laser digital photographic documentation were regularly performed to allow wound assessment including wound surface area. Full-thickness skin punch biopsy was performed at each site before wound induction (D0, normal skin) and after complete wound healing (D62, repaired skin). Results. All wounds healed without adverse effect at D62. Distal limb wounds are slower to heal than body wounds. OM-MSC and its secretome have a positive impact on TH wound contraction. OM-MSC has a positive impact on the contraction and epithelialization of FL wounds. No significant difference between wound sites before and after treatment was noted at histological examination. Conclusion and Clinical Relevance. Using horse cells harvested from oral mucosa is a feasible technique to produce OM-MSC or its secretome. The gel produced by the combination of these biologic components with HA shows a positive impact when applied during the early stage of wound healing.

Decellularized extracellular matrix microparticles seeded with bone marrow mesenchymal stromal cells for the treatment of full-thickness cutaneous wounds

2019 ◽  
Vol 33 (8) ◽  
pp. 1070-1079 ◽  
Author(s):  
Amanda M Westman ◽  
Rachel L Goldstein ◽  
Gino Bradica ◽  
Scott M Goldman ◽  
Mark A Randolph ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Full Thickness ◽  
Decellularized Extracellular Matrix ◽  
Wound Model ◽  
Cellular Attachment ◽  
Healing Environments

Extracellular matrix materials mechanically dissociated into submillimeter particles have a larger surface area than sheet materials and enhanced cellular attachment. Decellularized porcine mesothelial extracellular matrix microparticles were seeded with bone marrow-derived mesenchymal stromal cells and cultured in a rotating bioreactor. The mesenchymal stromal cells attached and grew to confluency on the microparticles. The cell-seeded microparticles were then encapsulated in varying concentrations of fibrin glue, and the cells migrated rapidly off the microparticles. The combination of microparticles and mesenchymal stromal cells was then applied to a splinted full-thickness cutaneous in vivo wound model. There was evidence of increased cell infiltration and collagen deposition in mesenchymal stromal cells-treated wounds. Cell-seeded microparticles have potential as a cell delivery and paracrine therapy in impaired healing environments.

Mesenchymal Stromal Cells Within Fibrin Gel Stimulate Healing of Full-Thickness Wounds in Mice

2017 ◽  
Vol 27 (2) ◽  
pp. 171-171
Author(s):  
Olga A. Tykhvynska ◽  
◽  
Olena Yu. Rogulska ◽  
Yuriy A. Petrenko ◽  
◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Full Thickness ◽  
Fibrin Gel
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