scholarly journals Composition-function relations of cartilaginous tissues engineered from chondrocytes and mesenchymal stem cells isolated from bone marrow and infrapatellar fat pad

2010 ◽  
Vol 5 (9) ◽  
pp. 673-683 ◽  
Author(s):  
T. Vinardell ◽  
C. T. Buckley ◽  
S. D. Thorpe ◽  
D. J. Kelly
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Infrapatellar Fat Pad ◽  
Fat Pad ◽  
Cartilaginous Tissues

scholarly journals Canine mesenchymal stem cells from synovium have a higher chondrogenic potential than those from infrapatellar fat pad, adipose tissue, and bone marrow

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0202922 ◽  
Author(s):  
Akari Sasaki ◽  
Mitsuru Mizuno ◽  
Nobutake Ozeki ◽  
Hisako Katano ◽  
Koji Otabe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Infrapatellar Fat Pad ◽  
Fat Pad ◽  
Chondrogenic Potential

scholarly journals Functional properties of cartilaginous tissues engineered from infrapatellar fat pad-derived mesenchymal stem cells

2010 ◽  
Vol 43 (5) ◽  
pp. 920-926 ◽  
Author(s):  
Conor Timothy Buckley ◽  
Tatiana Vinardell ◽  
Stephen Desmond Thorpe ◽  
Matthew George Haugh ◽  
Elena Jones ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Functional Properties ◽  
Infrapatellar Fat Pad ◽  
Fat Pad ◽  
Cartilaginous Tissues

scholarly journals Chondrogenic Potency Analyses of Donor-Matched Chondrocytes and Mesenchymal Stem Cells Derived from Bone Marrow, Infrapatellar Fat Pad, and Subcutaneous Fat

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
John Garcia ◽  
Claire Mennan ◽  
Helen S. McCarthy ◽  
Sally Roberts ◽  
James B. Richardson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cartilage Repair ◽  
Subcutaneous Fat ◽  
Cell Types ◽  
Infrapatellar Fat Pad ◽  
Fat Pad ◽  
Cell Based Therapy ◽  
Chondrogenic Potential

Autologous chondrocyte implantation (ACI) is a cell-based therapy that has been used clinically for over 20 years to treat cartilage injuries more efficiently in order to negate or delay the need for joint replacement surgery. In this time, very little has changed in the ACI procedure, but now many centres are considering or using alternative cell sources for cartilage repair, in particular mesenchymal stem cells (MSCs). In this study, we have tested the chondrogenic potential of donor-matched MSCs derived from bone marrow (BM), infrapatellar fat pad (FP), and subcutaneous fat (SCF), compared to chondrocytes. We have confirmed that there is a chondrogenic potency hierarchy ranging across these cell types, with the most potent being chondrocytes, followed by FP-MSCs, BM-MSCs, and lastly SCF-MSCs. We have also examined gene expression and surface marker profiles in a predictive model to identify cells with enhanced chondrogenic potential. In doing so, we have shown that Sox-9, Alk-1, and Coll X expressions, as well as immunopositivity for CD49c and CD39, have predictive value for all of the cell types tested in indicating chondrogenic potency. The findings from this study have significant clinical implications for the refinement and development of novel cell-based cartilage repair strategies.

scholarly journals Recent Advance in Source, Property, Differentiation, and Applications of Infrapatellar Fat Pad Adipose-Derived Stem Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-14 ◽  
Author(s):  
Yu-chen Zhong ◽  
Shi-chun Wang ◽  
Yin-he Han ◽  
Yu Wen
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Regenerative Medicine ◽  
3D Culture ◽  
Infrapatellar Fat Pad ◽  
Adipose Derived Stem Cells ◽  
Fat Pad ◽  
Stem Cell Source ◽  
Marrow Mesenchymal Stem Cells ◽  
And Function

Infrapatellar fat pad (IPFP) can be easily obtained during knee surgery, which avoids the damage to patients for obtaining IPFP. Infrapatellar fat pad adipose-derived stem cells (IPFP-ASCs) are also called infrapatellar fat pad mesenchymal stem cells (IPFP-MSCs) because the morphology of IPFP-ASCs is similar to that of bone marrow mesenchymal stem cells (BM-MSCs). IPFP-ASCs are attracting more and more attention due to their characteristics suitable to regenerative medicine such as strong proliferation and differentiation, anti-inflammation, antiaging, secreting cytokines, multipotential capacity, and 3D culture. IPFP-ASCs can repair articular cartilage and relieve the pain caused by osteoarthritis, so most of IPFP-related review articles focus on osteoarthritis. This article reviews the anatomy and function of IPFP, as well as the discovery, amplification, multipotential capacity, and application of IPFP-ASCs in order to explain why IPFP-ASC is a superior stem cell source in regenerative medicine.

scholarly journals The Effect of Cyclic Hydrostatic Pressure on the Functional Development of Cartilaginous Tissues Engineered Using Bone Marrow Derived Mesenchymal Stem Cells

2011 ◽  
Author(s):  
Eric G. Meyer ◽  
Conor T. Buckley ◽  
Daniel J. Kelly
Keyword(s):  
Mechanical Properties ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Cartilaginous Tissues ◽  
Functional Development ◽  
The Many ◽  
Interesting Alternative

Articular cartilage has a poor capacity for repair. Of the many procedures available to the orthopaedic surgeon, osteochondral grafting is the only technique which reliably produces hyaline cartilage within a defect.1 Bone marrow derived mesenchymal stem cells (MSCs) are an interesting alternative to harvesting cartilage grafts for chondrocytes as they also have the ability to produce cartilaginous tissues in vitro. This suggests that if tissue engineering strategies could be used to develop cartilaginous grafts with mechanical properties approaching that of normal articular cartilage, then hyaline tissue could be regenerated. Of concern with such approaches are reports that the mechanical properties of cartilaginous tissues engineered using MSCs are inferior to that engineered using chondrocytes derived from articular cartilage, although recent studies have demonstrated that adult equine MSCs produce a cartilaginous tissue mechanically superior to that derived using animal-matched adult chondrocytes.2

scholarly journals The infrapatellar fat pad from diseased joints inhibits chondrogenesis of mesenchymal stem cells

2015 ◽  
Vol 23 ◽  
pp. A145-A146 ◽  
Author(s):  
W. Wei ◽  
R. Rudjito ◽  
N. Fahy ◽  
K.P. Bos ◽  
J.A. Verhaar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Infrapatellar Fat Pad ◽  
Fat Pad

scholarly journals Inability of Low Oxygen Tension to Induce Chondrogenesis in Human Infrapatellar Fat Pad Mesenchymal Stem Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Samia Rahman ◽  
Alexander R. A. Szojka ◽  
Yan Liang ◽  
Melanie Kunze ◽  
Victoria Goncalves ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Oxygen Tension ◽  
Endochondral Ossification ◽  
Infrapatellar Fat Pad ◽  
Low Oxygen ◽  
Fat Pad ◽  
Chondrogenic Medium ◽  
Low Oxygen Tension

ObjectiveArticular cartilage of the knee joint is avascular, exists under a low oxygen tension microenvironment, and does not self-heal when injured. Human infrapatellar fat pad-sourced mesenchymal stem cells (IFP-MSC) are an arthroscopically accessible source of mesenchymal stem cells (MSC) for the repair of articular cartilage defects. Human IFP-MSC exists physiologically under a low oxygen tension (i.e., 1–5%) microenvironment. Human bone marrow mesenchymal stem cells (BM-MSC) exist physiologically within a similar range of oxygen tension. A low oxygen tension of 2% spontaneously induced chondrogenesis in micromass pellets of human BM-MSC. However, this is yet to be demonstrated in human IFP-MSC or other adipose tissue-sourced MSC. In this study, we explored the potential of low oxygen tension at 2% to drive the in vitro chondrogenesis of IFP-MSC. We hypothesized that 2% O2 will induce stable chondrogenesis in human IFP-MSC without the risk of undergoing endochondral ossification at ectopic sites of implantation.MethodsMicromass pellets of human IFP-MSC were cultured under 2% O2 or 21% O2 (normal atmosphere O2) in the presence or absence of chondrogenic medium with transforming growth factor-β3 (TGFβ3) for 3 weeks. Following in vitro chondrogenesis, the resulting pellets were implanted in immunodeficient athymic nude mice for 3 weeks.ResultsA low oxygen tension of 2% was unable to induce chondrogenesis in human IFP-MSC. In contrast, chondrogenic medium with TGFβ3 induced in vitro chondrogenesis. All pellets were devoid of any evidence of undergoing endochondral ossification after subcutaneous implantation in athymic mice.

Sign in / Sign up

Export Citation Format

Share Document