Design of semi-degradable hydrogels based on poly(vinyl alcohol) and poly(lactic-co-glycolic acid) for cartilage tissue engineering

2010 ◽  
Vol 5 (8) ◽  
pp. 636-647 ◽  
Author(s):  
Kara L. Spiller ◽  
Julianne L. Holloway ◽  
Megan E. Gribb ◽  
Anthony M. Lowman
Keyword(s):  
Tissue Engineering ◽  
Vinyl Alcohol ◽  
Glycolic Acid ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Poly Vinyl Alcohol

Dynamic hyaluronic acid hydrogel with covalent linked gelatin as an anti-oxidative bioink for cartilage tissue engineering

2021 ◽  
Author(s):  
Wen Shi ◽  
Fang Fang ◽  
Yunfan Kong ◽  
Sydney E Greer ◽  
Mitchell A Kuss ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Therapeutic Option ◽  
Phenylboronic Acid ◽  
Cartilage Tissue Engineering ◽  
Covalent Bond ◽  
Cartilage Tissue ◽  
Poly Vinyl Alcohol ◽  
Extracellular Matrix Components

Abstract Cartilage tissue engineering has arisen as a promising therapeutic option for degenerative joint diseases, such as osteoarthritis, in the hope of restoring the structure and physiological functions. Hydrogels are promising biomaterials for developing engineered constructs for cartilage regeneration. However, such cell-laden constructs could be exposed to elevated levels of reactive oxygen species (ROS) in the inflammatory microenvironment after being implanted into injured joints, which may affect their phenotype and normal functions and thereby hinder the regeneration efficacy. To attenuate ROS induced side effects, a multifunctional hydrogel with an innate anti-oxidative ability was produced in this study. The hydrogel was rapidly formed through a dynamic covalent bond between phenylboronic acid grafted hyaluronic acid (HA-PBA) and poly (vinyl alcohol) (PVA) and was further stabilized through a secondary crosslinking between the acrylate moiety on HA-PBA and the free thiol group from thiolated gelatin. The hydrogel is cyto-compatible and injectable and can be used as a bioink for 3D bioprinting. The viscoelastic properties of the hydrogels could be modulated through the hydrogel precursor concentration. The presence of dynamic covalent linkages contributed to its shear-thinning property and thus good printability of the hydrogel, resulting in the fabrication of a porous grid construct and a meniscus like scaffold at high structural fidelity. The bioprinted hydrogel promoted cell adhesion and chondrogenic differentiation of encapsulated rabbit adipose derived mesenchymal stem cells. Meanwhile, the hydrogel supported robust deposition of extracellular matrix components, including glycosaminoglycans and type II collagen, by embedded mouse chondrocytes in vitro. Most importantly, the hydrogel could protect encapsulated chondrocytes from ROS induced downregulation of cartilage-specific anabolic genes (ACAN and COL2) and upregulation of a catabolic gene (MMP13) after incubation with H2O2. Furthermore, intra-articular injection of the hydrogel in mice revealed adequate stability and good biocompatibility in vivo. These results demonstrate that this hydrogel can be used as a novel bioink for the generation of 3D bioprinted constructs with anti-ROS ability to potentially enhance cartilage tissue regeneration in a chronic inflammatory and elevated ROS microenvironment.

Cartilage tissue engineering with controllable shape using a poly(lactic-co-glycolic acid)/collagen hybrid scaffold

2013 ◽  
Vol 28 (3) ◽  
pp. 247-257 ◽  
Author(s):  
Wenda Dai ◽  
Zhenjun Yao ◽  
Jian Dong ◽  
Naoki Kawazoe ◽  
Chi Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Glycolic Acid ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Hybrid Scaffold

scholarly journals Carboxymethyl Cellulose Entrapped in a Poly(vinyl) Alcohol Network: Plant-Based Scaffolds for Cartilage Tissue Engineering

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 578
Author(s):  
Jirapat Namkaew ◽  
Panitporn Laowpanitchakorn ◽  
Nuttapong Sawaddee ◽  
Sirinee Jirajessada ◽  
Sittisak Honsawek ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Carboxymethyl Cellulose ◽  
Cell Attachment ◽  
Cartilage Tissue Engineering ◽  
Crosslinking Density ◽  
Cartilage Tissue ◽  
Poly Vinyl Alcohol ◽  
Porous Scaffolds ◽  
Swelling Properties ◽  
Engineered Cartilage

Cartilage has a limited inherent healing capacity after injury, due to a lack of direct blood supply and low cell density. Tissue engineering in conjunction with biomaterials holds promise for generating cartilage substitutes that withstand stress in joints. A major challenge of tissue substitution is creating a functional framework to support cartilage tissue formation. Polyvinyl alcohol (PVA) was crosslinked with glutaraldehyde (GA), by varying the mole ratios of GA/PVA in the presence of different amounts of plant-derived carboxymethyl cellulose (CMC). Porous scaffolds were created by the freeze-drying technique. The goal of this study was to investigate how CMC incorporation and crosslinking density might affect scaffold pore formation, swelling behaviors, mechanical properties, and potential use for engineered cartilage. The peak at 1599 cm−1 of the C=O group in ATR–FTIR indicates the incorporation of CMC into the scaffold. The glass transition temperature (Tg) and Young’s modulus were lower in the PVA/CMC scaffold, as compared to the PVA control scaffold. The addition of CMC modulates the pore architecture and increases the swelling ratio of scaffolds. The toxicity of the scaffolds and cell attachment were tested. The results suggest that PVA/CMC scaffolding material can be tailored in terms of its physical and swelling properties to potentially support cartilage formation.

scholarly journals Adipose tissue-derived mesenchymal stem cells and chitosan/poly (vinyl alcohol) nanofibrous scaffolds for cartilage tissue engineering

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Ghada Nour-Eldeen ◽  
Mazen Abdel-Rasheed ◽  
Amira M. EL-Rafei ◽  
Osama Azmy ◽  
Gehan T. El-Bassyouni
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Vinyl Alcohol ◽  
Cartilage Tissue ◽  
Poly Vinyl Alcohol ◽  
Differentiation Potential ◽  
Nanofibrous Scaffolds ◽  
Scanning Electron

AbstractOsteoarthritis (OA) has been defined as a chronic inflammatory joint disease characterized by progressive articular cartilage degeneration. Recently growing interest in regenerative medicine, using cell therapy and tissue engineering, where cellular components in combination with engineered scaffolds and bioactive materials were used to induce functional tissue regeneration. In the present study, nanofibrous scaffold based on chitosan (CS)/poly (vinyl alcohol) (PVA) were used to develop biologically functionalized biomaterial to mimic the extracellular matrix, allowing the human adipose tissue derived mesenchymal stem cells (ADSCs) to proliferate and differentiate to chondrogenic cells. The morphology of the nanofibrous mat was examined using field emission scanning electron microscope (FE/SEM). The characteristic functional groups and the nature of the chemical bonds between atoms were evaluated using Fourier transform infrared spectroscopy (FTIR) spectrum. Characterization of the seeded cells was morphologically evaluated by scanning electron microscopy and by flow cytometry for the expression of the stem cell surface markers. The differentiation potential was verified after chondrogenic induction by analyzing the expression of chondrogenic marker genes using real-time (RT PCR). Current study suggest significant potential for the use of ADSCs with the nanofibrous scaffolds in improving the osteoarthritis pathology.

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