Enhanced osteoblastic differentiation of mesenchymal stem cells seeded in RGD-functionalized PLLA scaffolds and cultured in a flow perfusion bioreactor

2010 ◽  
Vol 5 (6) ◽  
pp. 464-475 ◽  
Author(s):  
Jose F. Alvarez-Barreto ◽  
Bonnie Landy ◽  
Samuel VanGordon ◽  
Laura Place ◽  
Paul L. DeAngelis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteoblastic Differentiation ◽  
Perfusion Bioreactor ◽  
Flow Perfusion ◽  
Flow Perfusion Bioreactor

scholarly journals Uncarboxylated osteocalcin alleviates the inhibitory effect of high glucose on osteogenic differentiation of mouse bone marrow–derived mesenchymal stem cells by regulating TP63

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Fangzi Gong ◽  
Le Gao ◽  
Luyao Ma ◽  
Guangxin Li ◽  
Jianhong Yang
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
High Glucose ◽  
Bone Development ◽  
Inhibitory Effect ◽  
Osteoblastic Differentiation ◽  
Diabetic Patients ◽  
Mouse Bone Marrow

Abstract Background Progressive population aging has contributed to the increased global prevalence of diabetes and osteoporosis. Inhibition of osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by hyperglycemia is a potential pathogenetic mechanism of osteoporosis in diabetic patients. Uncarboxylated osteocalcin (GluOC), a protein secreted by mature osteoblasts, regulates bone development as well as glucose and lipid metabolism. In our previous studies, GluOC was shown to promote osteoblastic differentiation of BMSCs; however, the underlying mechanisms are not well characterized. Tumor protein 63 (TP63), as a  transcription factor, is closely related to bone development and glucose metabolism. Results In this study, we verified that high glucose suppressed osteogenesis and upregulated adipogenesis in BMSCs, while GluOC alleviated this phenomenon. In addition, high glucose enhanced TP63 expression while GluOC diminished it. Knock-down of TP63 by siRNA transfection restored the inhibitory effect of high glucose on osteogenic differentiation. Furthermore, we detected the downstream signaling pathway PTEN/Akt/GSK3β. We found that diminishing TP63 decreased PTEN expression and promoted the phosphorylation of Akt and GSK3β. We then applied the activator and inhibitor of Akt, and concluded that PTEN/Akt/GSK3β participated in regulating the differentiation of BMSCs. Conclusions Our results indicate that GluOC reduces the inhibitory effect of high glucose on osteoblast differentiation by regulating the TP63/PTEN/Akt/GSK3β pathway. TP63 is a potential novel target for the prevention and treatment of diabetic osteoporosis.

Intranuclear mesoscale viscoelastic changes during osteoblastic differentiation of human mesenchymal stem cells

2021 ◽  
Vol 35 (12) ◽  
Author(s):  
Kojiro Matsushita ◽  
Chiharu Nakahara ◽  
Shun Kimura ◽  
Naoya Sakamoto ◽  
Satoshi Ii ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Osteoblastic Differentiation

Gene expression of TWIST1 and ZBTB16 is regulated by methylation modifications during the osteoblastic differentiation of mesenchymal stem cells

2018 ◽  
Vol 234 (5) ◽  
pp. 6230-6243 ◽  
Author(s):  
Faroogh Marofi ◽  
Ghasem Vahedi ◽  
Saeed Solali ◽  
Mohammadreza Alivand ◽  
Sadegh Salarinasab ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteoblastic Differentiation

Recovering the osteoblastic differentiation potential of mesenchymal stem cells derived from diabetic rats by photobiomodulation therapy

2020 ◽  
Author(s):  
Natália Pieretti Bueno ◽  
Isabella Nunes Copete ◽  
Helena Bacha Lopes ◽  
Praveen R. Arany ◽  
Márcia Martins Marques ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteoblastic Differentiation ◽  
Diabetic Rats ◽  
Photobiomodulation Therapy ◽  
Differentiation Potential

Distinct osteoblastic differentiation potential of murine fetal liver and bone marrow stroma-derived mesenchymal stem cells

2008 ◽  
Vol 104 (2) ◽  
pp. 620-628 ◽  
Author(s):  
Olivia Fromigué ◽  
Zahia Hamidouche ◽  
Sébastien Chateauvieux ◽  
Pierre Charbord ◽  
Pierre J. Marie
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Fetal Liver ◽  
Osteoblastic Differentiation ◽  
Bone Marrow Stroma ◽  
Differentiation Potential ◽  
Marrow Stroma
Sign in / Sign up

Export Citation Format

Share Document