scholarly journals Efficient in vivo vascularization of tissue-engineering scaffolds

2010 ◽  
Vol 5 (4) ◽  
pp. e52-e62 ◽  
Author(s):  
Anja Hegen ◽  
Anna Blois ◽  
Crina E. Tiron ◽  
Monica Hellesøy ◽  
David R. Micklem ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Tissue Engineering Scaffolds

Preparation, Characterization and In Vitro Release Kinetics of Vancomycin Carried β-TCP/Chitosan Composite Microspheres Used as Bone Tissue Engineering Scaffolds

2012 ◽  
Vol 512-515 ◽  
pp. 1821-1825
Author(s):  
Lin Zhang ◽  
Xue Min Cui ◽  
Qing Feng Zan ◽  
Li Min Dong ◽  
Chen Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Release Kinetics ◽  
In Vitro Release ◽  
Cross Linking ◽  
Tissue Engineering Scaffolds ◽  
Composite Microspheres ◽  
Chitosan Composite ◽  
Vitro Release

A novel microsphere scaffolds composed of chitosan and β-TCP containing vancomycin was designed and prepared. The β-TCP/chitosan composite microspheres were prepared by solid-in-water-in-oil (s/w/o) emulsion cross-linking method with or without pre-cross-linking process. The mode of vancomycin maintaining in the β-TCP/chitosan composite microspheres was detected by Fourier transform infrared spectroscopy (FTIR). The in vitro release curve of vancomycin in simulated body fluid (SBF) was estimated. The results revealed that the pre-cross-linking prepared microspheres possessed higher loading efficiency (LE) and encapsulation efficiency (EE) especially decreasing the previous burst mass of vancomycin in incipient release. These composite microspheres got excellent sphere and well surface roughness in morphology. Vancomycin was encapsulated in composite microspheres through absorption and cross-linking. While in-vitro release curves illustrated that vancomycin release depond on diffusing firstly and then on the degradation ratio later. The microspheres loading with vancomycin would be to restore bone defect, meanwhile to inhibit bacterium proliferation. These bioactive, degradable composite microspheres have potential applications in 3D tissue engineering of bone and other tissues in vitro and in vivo.

scholarly journals Feasibility of Spatially Offset Raman Spectroscopy for in Vitro and in Vivo Monitoring Mineralization of Bone Tissue Engineering Scaffolds

2016 ◽  
Vol 89 (1) ◽  
pp. 847-853 ◽  
Author(s):  
Zhiyu Liao ◽  
Faris Sinjab ◽  
Amy Nommeots-Nomm ◽  
Julian Jones ◽  
Laura Ruiz-Cantu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Raman Spectroscopy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Engineering Scaffolds ◽  
In Vivo Monitoring

Biodegradable Microfluidic Scaffolds with Tunable Degradation Properties from Amino Alcohol-based Poly(ester amide) Elastomers

2011 ◽  
Vol 1299 ◽  
Author(s):  
Jane Wang ◽  
Tatiana Kniazeva ◽  
Carly F. Campbell ◽  
Robert Langer ◽  
Jeffrey S. Ustin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Biodegradable Polymers ◽  
Half Life ◽  
Microfluidic Devices ◽  
Gas Permeation ◽  
Microfluidic Channels ◽  
Tissue Engineering Scaffolds ◽  
Rapid Prototyping And Manufacturing ◽  
Degradation Properties

ABSTRACTBiodegradable polymers with high mechanical strength, flexibility and optical transparency, optimal degradation properties and biocompatibility are critical to the success of tissue engineered devices and drug delivery systems. In this work, microfluidic devices have been fabricated from elastomeric scaffolds with tunable degradation properties for applications in tissue engineering and regenerative medicine. Most biodegradable polymers suffer from short half life resulting from rapid and poorly controlled degradation upon implantation, exceedingly high stiffness, and limited compatibility with chemical functionalization. Here we report the first microfluidic devices constructed from a recently developed class of biodegradable elastomeric poly(ester amide)s, poly(1,3-diamino-2-hydroxypropane-co-polyol sebacate)s (APS), showing a much longer and highly tunable in vivo degradation half-life comparing to many other commonly used biodegradable polymers. The device is molded in a similar approach to that reported previously for conventional biodegradable polymers, and the bonded microfluidic channels are shown to be capable of supporting physiologic levels of flow and pressure. The device has been tested for degradation rate and gas permeation properties in order to predict performance in the implantation environment. This device is high resolution and fully biodegradable; the fabrication process is fast, inexpensive, reproducible, and scalable, making it the approach ideal for both rapid prototyping and manufacturing of tissue engineering scaffolds and vasculature and tissue and organ replacements.

scholarly journals Novel layered double hydroxides-hydroxyapatite/gelatin bone tissue engineering scaffolds: Fabrication, characterization, and in vivo study

2017 ◽  
Vol 76 ◽  
pp. 701-714 ◽  
Author(s):  
Fateme Fayyazbakhsh ◽  
Mehran Solati-Hashjin ◽  
Abbas Keshtkar ◽  
Mohammad Ali Shokrgozar ◽  
Mohammad Mehdi Dehghan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Layered Double Hydroxides ◽  
In Vivo Study ◽  
Tissue Engineering Scaffolds ◽  
Double Hydroxides

A Brief Review of the Modelling of the Time Dependent Mechanical Properties of Tissue Engineering Scaffolds

2010 ◽  
Vol 6 ◽  
pp. 19-33 ◽  
Author(s):  
N.K. Bawolin ◽  
W.J. Zhang ◽  
Xiong Biao Chen
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Treatment Process ◽  
Effective Properties ◽  
Critical Role ◽  
Time Dependent ◽  
Tissue Engineering Scaffolds ◽  
Wide Range ◽  
Scaffold Degradation

The functionality of tissue scaffolds in vivo plays a critical role in the treatment process. Due to the time dependent nature of the mechanical properties of the constituent phases of the scaffold, a wide range of mechanical property histories may be observed during the treatment process, possibly influencing outcomes. The critical nature of the mechanical properties in load bearing applications indicates a need for the simultaneous modelling of both scaffold degradation and tissue regeneration with time, and the resulting effective properties of the tissue engineering construct. To this end, a review of the literature is conducted to identify the various existing approaches to modelling scaffold degradation, tissue behavior, and the dependency of the two processes on one another.

scholarly journals A Systematic Review of Tissue Engineering Scaffold in Tendon Bone Healing in vivo

2021 ◽  
Vol 9 ◽  
Author(s):  
Zimu Mao ◽  
Baoshi Fan ◽  
Xinjie Wang ◽  
Ximeng Huang ◽  
Jian Guan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Healing ◽  
Animal Studies ◽  
Ligament Reconstruction ◽  
Search Strategy ◽  
Web Of Science ◽  
Tissue Engineering Scaffold ◽  
Tissue Engineering Scaffolds ◽  
Eligibility Criteria

Background: Tendon-bone healing is an important factor in determining the success of ligament reconstruction. With the development of biomaterials science, the tissue engineering scaffold plays an extremely important role in tendon-bone healing and bone tissue engineering.Materials and Methods: Electronic databases (PubMed, Embase, and the Web of Science) were systematically searched for relevant and qualitative studies published from 1 January 1990 to 31 December 2019. Only original articles that met eligibility criteria and evaluated the use of issue engineering scaffold especially biomaterials in tendon bone healing in vivo were selected for analysis.Results: The search strategy identified 506 articles, and 27 studies were included for full review including two human trials and 25 animal studies. Fifteen studies only used biomaterials like PLGA, collage, PCL, PLA, and PET as scaffolds to repair the tendon-bone defect, on this basis, the rest of the 11 studies using biological interventions like cells or cell factors to enhance the healing. The adverse events hardly ever occurred, and the tendon bone healing with tissue engineering scaffold was effective and superior, which could be enhanced by biological interventions.Conclusion: Although a number of tissue engineering scaffolds have been developed and applied in tendon bone healing, the researches are mainly focused on animal models which are with limitations in clinical application. Since the efficacy and safety of tissue engineering scaffold has been proved, and can be enhanced by biological interventions, substantial clinical trials remain to be done, continued progress in overcoming current tissue engineering challenges should allow for successful clinical practice.

scholarly journals Scaffold-Based Tissue Engineering Strategies for Osteochondral Repair

2022 ◽  
Vol 9 ◽  
Author(s):  
Jiang-Nan Fu ◽  
Xing Wang ◽  
Meng Yang ◽  
You-Rong Chen ◽  
Ji-Ying Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Metal Composite ◽  
Tissue Engineering Scaffolds ◽  
Advantages And Disadvantages ◽  
Osteochondral Repair ◽  
Cell Growth And Differentiation ◽  
Manufacturing Technologies ◽  
Made In

Over centuries, several advances have been made in osteochondral (OC) tissue engineering to regenerate more biomimetic tissue. As an essential component of tissue engineering, scaffolds provide structural and functional support for cell growth and differentiation. Numerous scaffold types, such as porous, hydrogel, fibrous, microsphere, metal, composite and decellularized matrix, have been reported and evaluated for OC tissue regeneration in vitro and in vivo, with respective advantages and disadvantages. Unfortunately, due to the inherent complexity of organizational structure and the objective limitations of manufacturing technologies and biomaterials, we have not yet achieved stable and satisfactory effects of OC defects repair. In this review, we summarize the complicated gradients of natural OC tissue and then discuss various osteochondral tissue engineering strategies, focusing on scaffold design with abundant cell resources, material types, fabrication techniques and functional properties.

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