LncRNA MALAT1 promotes osteogenic differentiation of BMSCs and inhibits osteoclastic differentiation of Mø in osteoporosis via the miR‐124‐3p/IGF2BP1/Wnt/β‐catenin axis

2021 ◽  
Author(s):  
Xiangxin Li
Keyword(s):  
Osteogenic Differentiation ◽  
Lncrna Malat1 ◽  
Osteoclastic Differentiation

scholarly journals lncRNA MALAT1 Mediates Osteogenic Differentiation and Apoptosis in Osteoporosis by Regulating the miR-485-5p/WNT7B Axis

2021 ◽  
Author(s):  
Yuan Zhou ◽  
Zhuo Xu ◽  
Yuanyi Wang ◽  
Qiang Song ◽  
Ruofeng Yin
Keyword(s):  
Osteogenic Differentiation ◽  
Cell Apoptosis ◽  
Binding Sites ◽  
Hindlimb Unloading ◽  
Cellular Functions ◽  
Alizarin Red ◽  
Lncrna Malat1 ◽  
Non Coding Rnas

Abstract Background: Accumulating evidence demonstrates that long non-coding RNAs (lncRNAs) are associated with the development of osteoporosis. This study aimed to investigate the effects of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on osteogenic differentiation and cell apoptosis in osteoporosis. Methods: hindlimb unloading (HU) was performed to establish osteoporosis model in vivo. MicroCT was applied for pathological analysis. Microgravity (MG) was used to construct osteoporosis in vitro. The mRNA and miRNA expression was determined using RT-qPCR. Protein expression was determined using western blot. The binding sites between miR-485-5p and MALAT1/Wnt family member 7B (WNT7B) was predicted by bioinformatics analysis and verified by luciferase and RNA pull-down assays. Cellular functions were determined by ALP staining, Alizarin red staining, and flow cytometry assays. Results: MALAT1 expression was downregulated in HU mice and MG treated MC3T3-E1 cells. However, overexpression of MALAT1 upregulated the expression of Bmp4, Col1a1, Spp1, and enhanced ALP activity. Additionally, overexpression of MALAT1 inhibited apoptosis, decreased Bax and caspase-3 levels, and increased Bcl-2 level. Moreover, MALAT1 overexpression improved bone phenotype in vivo. MALAT1 functioned as a ceRNA to upregulate WNT7B. Overexpression of miR-485-5p rescued the promotion of osteogenic differentiation and the inhibition of apoptosis induced by MALAT1. Knockdown of WNT7B abolished the facilitation of osteogenic differentiation and the suppression of apoptosis induced by downregulation of miR-485-5p. Conclusion: In conclusion, MALAT1 promoted osteogenic differentiation and inhibited cell apoptosis through miR-485-5p/WNT7B axis, which suggested that MALAT1 is a potential target to alleviate osteoporosis.

γ-Aminobutyric Acid Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Inducing TNFAIP3

2020 ◽  
Vol 20 (2) ◽  
pp. 152-161
Author(s):  
Hanwen Li ◽  
Yongyao Wu ◽  
Ning Huang ◽  
Qi Zhao ◽  
Quan Yuan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Unmet Need ◽  
Aminobutyric Acid ◽  
Cell Counting ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Mineral Density ◽  
Osteoclastic Differentiation

Background: Osteoporosis is the most common metabolic bone disease. There is still an unmet need for novel therapeutic agents that could be beneficial as osteoporosis treatments. It has been reported that the neurotransmitter γ-aminobutyric acid (GABA) might be associated with human bone formation. However, the precise mechanism remains unclear. Objective: To investigate the effect of GABA on bone metabolism and explore the possible role of TNFAIP3 in this process. Methods: GABA had little effect on the proliferation of human mesenchymal stem cells (hMSCs) and RAW 264.7 cells, as indicated by the cell counting kit-8 (CCK-8) assay. The results showed that GABA enhanced the intensity of ALP staining, ALP activity, and accumulation of Ca2+ mineralized nodules in hMSCs during osteogenic induction. Results: The qRT-PCR results indicated that GABA treatment significantly increased the mRNA expression of osteogenic genes in hMSCs. In RAW 264.7 cells, TRAP staining showed that GABA did not alter the number or size of osteoclasts or the expression of osteoclastic genes, which suggests that GABA does not affect osteoclastic differentiation. Mechanistically, GABA treatment significantly induced the sustained expression of TNFAIP3. Furthermore, by knocking down TNFAIP3, the osteogenic effect of GABA was antagonized, which suggests that TNFAIP3 mediates the effects of GABA in hMSCs. Conclusion: Our results suggested that GABA treatment positively regulated osteogenic differentiation by upregulating TNFAIP3, while no obvious effect on osteoclastic differentiation was detected. Therefore, our results provide a potential gene therapy for the treatment of osteoporosis and low bone mineral density.

scholarly journals Osteogenic Differentiation of Renal Interstitial Fibroblasts Promoted by lncRNA MALAT1 May Partially Contribute to Randall’s Plaque Formation

2021 ◽  
Vol 8 ◽  
Author(s):  
Zewu Zhu ◽  
Fang Huang ◽  
Weiping Xia ◽  
Huimin Zeng ◽  
Meng Gao ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Transcription Initiation ◽  
Potential Role ◽  
Ectopic Calcification ◽  
Competing Endogenous Rna ◽  
Runx2 Expression ◽  
Lncrna Malat1 ◽  
Current Belief ◽  
Rna Immunoprecipitation ◽  
Randall’S Plaques

BackgroundThe current belief is that Randall’s plaques (RP) constitute a nidus for the formation of idiopathic calcium oxalate stones, but the upstream events in RP formation remain unclear. The present study aimed to investigate whether RP formation shares similarities with biomineralization and to illustrate the potential role played by the lncRNA MALAT1 in osteogenic differentiation of human renal interstitial fibroblasts (hRIFs).Materials and MethodsBiomineralization and MALAT1 expression were assessed in RP, and hRIFs were isolated and induced under osteogenic conditions for further experiments. The transcription initiation and termination sites in MALAT1 were identified by 5′ and 3′ RACE. RNA immunoprecipitation assays and luciferase assays were used to validate the interactions among MALAT1, Runx2 and miRNAs.ResultsUpregulated expression of osteogenic markers and MALAT1 was observed in RP and hRIFs induced with osteogenic medium. Biomineralization in RP and calcium phosphate (CaP) deposits in induced hRIFs were further verified by electron microscopy. Furthermore, overexpression of MALAT1 promoted the osteogenic phenotype of hRIFs, while treatment with a miR-320a-5p mimic and knockdown of Runx2 significantly suppressed the osteogenic phenotype. Further analysis showed that MALAT1 functioned as a competing endogenous RNA to sponge miR-320a-5p, leading to upregulation of Runx2 and thus promoting osteogenic differentiation of hRIFs.ConclusionEctopic calcification and MALAT1 partially contributed to the formation of RP, in which MALAT1 might promote Runx2 expression to regulate osteogenic differentiation of hRIFs by sponging miRNA-320a-5p. The current study sheds new light on the lncRNA-directed mechanism of RP formation via a process driven by osteogenic-like cells.

Long Noncoding RNA Metastasis-Associated Lung Carcinoma Transcript 1 (MALAT1) Inhibits Bone Marrow Mesenchymal Stem Cell Formation Through Regulating Smad/Runx2 Signaling Pathway

2020 ◽  
Vol 10 (5) ◽  
pp. 730-736
Author(s):  
Qingyun Pan ◽  
Biao Dong ◽  
Yong He ◽  
Xiaohui Wang
Keyword(s):  
Bone Marrow ◽  
Cell Proliferation ◽  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Real Time Pcr ◽  
Caspase 3 ◽  
Control Group ◽  
Alp Activity ◽  
Lncrna Malat1 ◽  
Sirna Group

Osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is controlled by elaborate genetic programs. lncRNA MALAT1 plays an important role in many diseases. However, the role of lncRNA MALAT1 in BMSCs remains unclear. Isolated Rat BMSCs were cultured and randomly divided into control group, MALAT1 group and MALAT1 siRNA group, in which lncRNA MALAT1 plasmid and lncRNA MALAT1 siRNA were transfected into BMSCs followed by analysis of lncRNA MALAT1 expression by real time PCR, cell proliferation by MTT assay, Caspase 3 activity, ALP activity was analyzed, calcified nodules by alizarin red staining, expression of Smad1 and Smad7 by Western blot as well as Runx2 expression by real time PCR. In MALAT1 group, MALAT1 expression was significantly increased along with significantly inhibited cell proliferation, increased Caspase 3 activity, decreased ALP activity and calcified nodules, reduced expression of Smad1, Smad7 and Runx2 compared with control group (P < 0 05). MALAT1 expression in MALAT1 siRNA group was decreased with significantly promoted cell proliferation, decreased Caspase 3 activity, increased ALP activity and calcified nodules, as well as significantly elevated expression of Smad1, Smad7 and Runx2 compared with control group (P < 0 05). Up-regulation of lncRNA MALAT1 expression inhibits the Smad/Runx2 signaling pathway, thereby inhibiting BMSCs proliferation and osteogenesis. Down-regulation of lncRNA MALAT1 expression promotes Smad/Runx2 signaling pathway activation, inhibits BMSCs apoptosis, promotes proliferation and osteogenic differentiation.

lncRNA MALAT1 Inhibits Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells by Down-Regulating WNT5A

2019 ◽  
Vol 9 (11) ◽  
pp. 1520-1527
Author(s):  
Xiaoliang Li ◽  
Guofeng Xia ◽  
Hongmei Xin ◽  
Chunsheng Tao ◽  
Weiwei Lai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Wnt Signaling Pathway ◽  
Luciferase Reporter ◽  
Protein Levels ◽  
Lncrna Malat1 ◽  
Marrow Mesenchymal Stem Cells ◽  
Osteogenic Induction

ncRNA involves in osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). WNT5A participates in the growth and development of osteogenic differentiation. This study aims to investigate whether lncRNA MALAT1 regulates BMSCs osteogenesis through WNT5A. qRT-PCR was done to detect the lncRNA MALAT1 level and osteogenic markers in osteoporosis patients and control groups. The above markers and WNT5A protein levels were detected by Western blot. The degree of osteogenic differentiation was detected by ALP activity assay and ALP staining. The differentiation ability of BMSCs after lncRNA MALAT1 overexpression was detected by ARS staining. The binding site of lncRNA MALAT1 to WNT5A was determined by dual luciferase reporter assay. lncRNA MALAT1 expression was higher in patients with osteoporosis, and decreased significantly with increased osteogenic induction. Overexpression of lncRNA MALAT1 in BMSCS reduced WNT5A level, while interference with lncRNA MALAT1 increased WNT5A levels. In cells with overexpression of lncRNA MALAT1, transfection of si-WNT5A can significantly downregulate the RUNX2, OSX, ALP, OCN, OPN, and COL1A1, thereby inhibiting osteogenic differentiation, interfering with the regulation of WNT signaling pathway and regulating BMSCs osteogenic differentiation. lncRNA MALAT1 and WNT5A can regulate BMSCs osteogenesis, thus accelerating the progression of osteoporosis.

Icaritin promotes osteogenic differentiation while inhibits osteoclastic differentiation in vitro

Bone ◽  
2010 ◽  
Vol 47 ◽  
pp. S426 ◽  
Author(s):  
Dong Yao ◽  
Xinluan Wang ◽  
Xinhui Xie ◽  
Ge Zhang ◽  
Ling Qin
Keyword(s):  
Osteogenic Differentiation ◽  
Osteoclastic Differentiation
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